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Ovulation-inducing factor (OIF) is a protein present in llama seminal plasma that has recently been identified as beta-Nerve Growth Factor (NGF) and it induces not only a high rate of ovulation but also appears to have luteotrophic properties in this species. A 2-by-2 experimental design was used...


Silva Jiménez, MauricioUlloa Leal, C.Norambuena, C.Fernandez, A.Adams, G. P.Ratto, Marcelo[Ovulation-inducing factor (OIF/NGF) from seminal plasma origin enhances Corpus Luteum function in llamas regardless the preovulatory follicle diameter]Ovulation-inducing factor (OIF/NGF) from seminal plasma origin enhances Corpus Luteum function in llamas regardless the preovulatory follicle diameter

The objective of the study was to compare the pituitary and ovarian responses after intramuscular, intravenous, or intrauterine administration of P-nerve growth factor (beta-NGF) of seminal plasma origin (SP-NGF) in llamas. In experiment 1, mature female llamas with a growing follicle of 7 mm or ...


Silva Jiménez, MauricioFernandez, A.Ulloa Leal, C.Adams, G. P.Berland Olea, MarcoRatto, Marcelo[LH release and ovulatory response after intramuscular, intravenous, and intrauterine administration of beta-nerve growth factor of seminal plasma origin in female llamas]LH release and ovulatory response after intramuscular, intravenous, and intrauterine administration of beta-nerve growth factor of seminal plasma origin in female llamas

The objectives of this study were (1) to determine the effect of rabbit seminal plasma on LH secretion and ovulation using the llama animal model as an in vivo ovulation bioassay and (2) to determine the effect of llama or rabbit seminal plasma on ovulation induction in the rabbit model. In Exper...


Silva Jiménez, MauricioNino, A.Guerra, M.Letelier, C.Valderrama, X. P.Adams, G. P.Ratto, Marcelo[Is an ovulation-inducing factor (OIF) present in the seminal plasma of rabbits?]Is an ovulation-inducing factor (OIF) present in the seminal plasma of rabbits?

The objectives of the study were to determine the effects of nutritional restriction on ovarian function in llamas. Mature female llamas were assigned randomly to a Control group, fed 100% of maintenance energy requirements (MER) (n = 8), or a Restricted group (n = 8) fed from 70% to 40% of MER u...


Norambuena, M. C.Silva Jiménez, MauricioUrra, F.Ulloa Leal, C.Fernandez, A.Adams, G. P.Huanca, W.Ratto, Marcelo[Effects of nutritional restriction on metabolic, endocrine, and ovarian function in llamas (Lama glama)]Effects of nutritional restriction on metabolic, endocrine, and ovarian function in llamas (Lama glama)

The objectives of the study were to determine the effect of seminal plasma beta-NGF on Corpus Luteum morphology and function and level of mRNA expression of steroidogenic enzymes. Llamas were assigned (n = 12/per group) to receive an intramuscular dose of: (a) 1 ml phosphate buffered saline (PBS)...


Silva Jiménez, MauricioUlloa Leal, C.Valderrama, X. P.Bogle, O. A.Adams, G. P.Ratto, Marcelo[Nerve growth factor from seminal plasma origin (sp beta-NGF) increases CL vascularization and level of mRNA expression of steroidogenic enzymes during the early stage of Corpus Luteum development in llamas]Nerve growth factor from seminal plasma origin (sp beta-NGF) increases CL vascularization and level of mRNA expression of steroidogenic enzymes during the early stage of Corpus Luteum development in llamas

This study was designed to: 1) characterize the effect of ovulation-inducing factor (OIF) on pituitary LH secretion in ovariectomized (OVX) llamas; and 2) determine the effect of OIF on LH secretion in OVX llamas pretreated with estradiol-17 beta (E-17 beta) or estradiol benzoate (EB). In Experim...


Silva Jiménez, MauricioRecabarren, M. P.Recabarren, S. E.Adams, G. P.Ratto, Marcelo[Ovarian estradiol modulates the stimulatory effect of ovulation-inducing factor (OIF) on pituitary LH secretion in llamas]Ovarian estradiol modulates the stimulatory effect of ovulation-inducing factor (OIF) on pituitary LH secretion in llamas

The objective was to evaluate the developmental competence of cumulus-oocyte complexes (COC) collected by follicular aspiration in llamas treated with FSH or eCG. Llamas were assigned randomly to two groups (n = 16 per group) and treated, at the time of ovarian follicular wave emergence, with eit...


Berland Olea, Marcovon Baer, A.Ruiz, J.Parraguez, V. H.Morales, P.Adams, G. P.Ratto, Marcelo[In vitro fertilization and development of cumulus oocytes complexes collected by ultrasound-guided follicle aspiration in superstimulated llamas]In vitro fertilization and development of cumulus oocytes complexes collected by ultrasound-guided follicle aspiration in superstimulated llamas

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