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We developed and modified a precise, rapid, and reproducible protocol isolating high‐quality RNA from tissues of multiple varieties of cassava plants (Manihot esculenta Crantz). The resulting method is suitable for use in mini, midi, and maxi preparations and rapidly achieves high total RNA yield...
Past research developed reliable equations to base selections for high β-carotene on near-infrared spectroscopy (NIR) predictions (100 genotypes d−1) rather than with high-performance liquid chromatography (HPLC) (<10 samples d−1). During recent harvest, CIAT made selections based on NIR predicti...
Abstract Background Cassava whitefly outbreaks were initially reported in East and Central Africa cassava (Manihot esculenta Crantz) growing regions in the 1990’s and have now spread to other geographical locations, becoming a global pest severely affecting farmers and smallholder income. Whitefl...
In cassava, as for other major crops, molecular markers can reveal the genetic basis for both qualitative and quantitative phenotypic changes. This chapter examines the genetic diversity of cassava, the process of marker-assisted selection (MAS) and the application of genome sequencing to cassava...
It is widely recognized that increasing agricultural production to the levels needed to feed an expanding world population requires sharply increased public investment in research and development and widespread adoption of new technologies, but funding for national and international agricultural ...
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