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Several tannin-tolerant bacteria were isolated from enrichment cultures of rumen microflora of bush duiker, giraffe, Grant's gazelle, sheep, and goat, and established in medium containing crude tannin extracts or tannic acid. The isolates were characterized by classical and molecular methods. The...


Odenyo, A.A.Bishop, Richard P.Asefa, G.Jamnadass, Ramni H.Odongo, D.Osuji, P.O.[Characterization of tannin-tolerant bacterial isolates from East African ruminants]Characterization of tannin-tolerant bacterial isolates from East African ruminants

Polymorphic, multicopy gene families are frequently located in subtelomeric regions of the genomes of parasitic protozoa. Theileria parva telomere-associated (TA) DNA from two chromosomes contained long open reading frames (ORFs) 54% identical at the N-termini whose ends were 2670 and 2680 bp fro...


Bishop, Richard P.Gobright, E.I.Nene, VishvanathMorzaria, S.P.Musoke, A.J.Sohanpal, B.K.[Polymorphic open reading frames encoding secretory proteins are located less than 3 kilobases from Theileria parva telomeres]Polymorphic open reading frames encoding secretory proteins are located less than 3 kilobases from Theileria parva telomeres

Two distinct genes encoding single domain, ATP-binding cassette transport protein homologues of Theileria parva were cloned and sequenced. Neither of the genes is tandemly duplicated. One gene, TpABCI, encodes a predicted protein of 593 amino acids with an N-terminal hydrophobic domain containing...


Kibe, M.K.Macklin, M.D.Gobright, E.I.Bishop, Richard P.Urakawa, T.Ole-MoiYoi, O.K.[Characterisation of single domain ATP-binding cassette protein homologues of Theileria parva]Characterisation of single domain ATP-binding cassette protein homologues of Theileria parva

The 'Muguga cocktail' which is composed of three Theileria parva stocks Muguga. Kiambu 5 and Serengeti-transformed has been used extensively for live vaccination against East Coast fever in cattle in eastern, central and southern Africa. Herein we describe the molecular characterization of the T....


Bishop, Richard P.Geysen, D.Spooner, P.R.Skilton, Robert A.Nene, VishvanathDolan, T.T.Morzaria, S.P.[Molecular and immunological characterisation of Theileria parva stocks which are components of the Muguga cocktail used for vaccination against East Coast fever in cattle]Molecular and immunological characterisation of Theileria parva stocks which are components of the Muguga cocktail used for vaccination against East Coast fever in cattle

Two recombinant vaccines against Theileria parva, based on a near full-length version of the sporozoite surface antigen p67 (p67635), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one i...


Musoke, A.J.Rowlands, G.J.Nene, VishvanathNyanjui, J.Katende, J.Spooner, P.R.Mwaura, S.Odongo, D.Nkonge, C.Mbogo, S.Bishop, Richard P.Morzaria, S.P.[Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge]Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge

The nucleotide sequences of the large subunit ribosomal RNA coding genes within the two single copy ribosomal DNA transcription units of a cloned Theileria parva isolate from a buffalo were determined. The two LSU rRNA coding units differed by 11 nucleotide substitutions and two deletions of 1 an...


Bishop, Richard P.Gobright, E.I.Spooner, P.R.Allsopp, B.A.Sohanpal, B.K.Collins, N.[Microsequence heterogeneity and expression of LSU rRNA genes within the two single copy ribosomal transcription units of Theileria parva]Microsequence heterogeneity and expression of LSU rRNA genes within the two single copy ribosomal transcription units of Theileria parva

Ixodid ticks manipulate mammalian host pathways by secreting molecules from salivary glands. Novel cDNAs containing functional secretion signals were isolated from ixodid tick salivary glands using a signal sequence trap. Only 15/61 Rhipicephalus appendiculatus and 1/7 Amblyomma variegatum cDNAs ...


Lambson, B.Nene, VishvanathObura, M.Shah, TushaarPandit, P.Ole-MoiYoi, O.K.Delroux, K.Welburn, S.C.Skilton, Robert A.Villiers, Etienne P. deBishop, Richard P.[Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells]Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells

A stage-specific surface antigen of Theileria parva, p67, is the basis for the development of an anti-sporozoite vaccine for the control of East Coast fever (ECF) in cattle. By Pepscan analysis with a series of overlapping synthetic p67 peptides, the antigen was shown to contain five distinct lin...


Nene, VishvanathGobright, E.I.Bishop, Richard P.Morzaria, S.P.Musoke, A.J.[Linear peptide specificity of bovine antibody responses to p67 of Theileria parva and sequence diversity of sporozoite-neutralising epitopes: Implications for a vaccine]Linear peptide specificity of bovine antibody responses to p67 of Theileria parva and sequence diversity of sporozoite-neutralising epitopes: Implications for a vaccine
Bishop, Richard P.Baylis, H.Allsopp, B.A.Toye, Philip G.Nene, VishvanathDolan, T.T.Spooner, P.R.Kibe, M.Morzaria, S.P.[Genomic polymorphisms in Theileria parva]Genomic polymorphisms in Theileria parva

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