The transcriptional control of gene expression is not well documented in the Arthropoda. We describe transcriptional analysis of two exceptionally divergent homologues (Ra86) of the Bm86 gut antigen from Rhipicephalus appendiculatus. Bm86 forms the basis of a commercial vaccine for the control of...
Kamau, L.
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Skilton, Robert A.
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Odongo, David O.
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Mwaura, S.
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Githaka, Naftaly W.
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Kanduma, E.G.
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Obura, M.
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Kabiru, E.
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Orago, A.
,
Musoke, A.J.
,
Bishop, Richard P.
,
[Differential transcription of two highly divergent gut-expressed Bm86 antigen gene homologues in the tick Rhipicephalus appendiculatus (Acari: Ixodida)]
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Differential transcription of two highly divergent gut-expressed Bm86 antigen gene homologues in the tick Rhipicephalus appendiculatus (Acari: Ixodida)
We describe the generation of an auto-annotated index of genes that are expressed in the salivary glands of four-day fed female adult Rhipicephalus appendiculatus ticks. A total of 9162 EST sequences were derived from an uninfected tick cDNA library and 9844 ESTs were from a cDNA library from t...
Nene, Vishvanath
,
Lee, D.
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Kang'a, S.
,
Skilton, Robert A.
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Shah, Tushaar
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Villiers, Etienne P. de
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Mwaura, S.
,
Taylor, D.
,
Quackenbush, J.
,
Bishop, Richard P.
,
[Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva]
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Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva
Protein components of the cement cone of ixodid ticks are candidates for inclusion in vaccines against tick infestation, since they are essential for tick attachment and feeding. We describe here the cloning of a cDNA encoding a 36 kDa protein, designated Rhipicephalus Immuno-dominant Molecule 36...
Bishop, Richard P.
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Lambson, B.
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Wells, C.
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Pandit, P.
,
Osaso, J.
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Nkonge, C.
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Morzaria, S.P.
,
Musoke, A.J.
,
Nene, Vishvanath
,
[A cement protein of the tick Rhipicephalus appendiculatus, located in the secretory e cell granules of the type III salivary gland acini, induces strong antibody responses in cattle]
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A cement protein of the tick Rhipicephalus appendiculatus, located in the secretory e cell granules of the type III salivary gland acini, induces strong antibody responses in cattle
Theileria parva is the etiologic agent of East Coast fever, an economically important disease of cattle in sub-Saharan Africa. This protozoan parasite is biologically transmitted by Rhipicephalus appendiculatus (Neumann) (Acari: Ixodidae). An understanding of the vector—parasite interaction may a...
Odongo, David O.
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Ueti, M.W.
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Mwaura, S.N.
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Knowles, Donald P.
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Bishop, Richard P.
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Scoles, Glen A.
,
[Quantification of Theileria parva in Rhipicephalus appendiculatus (Acari: Ixodidae) Confirms differences in infection between selected tick strains]
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Quantification of Theileria parva in Rhipicephalus appendiculatus (Acari: Ixodidae) Confirms differences in infection between selected tick strains
Theileria are economically important, intra-cellular protozoa, transmitted by ixodid ticks, which infect wild and domestic ruminants. In the mammalian host, parasites infect leukocytes and erythrocytes. In the arthropod vector they develop in gut epithelial cells and salivary glands. All four int...
Bishop, Richard P.
,
Musoke, A.J.
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Morzaria, S.P.
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Gardner, M.
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Nene, Vishvanath
,
[Theileria: Intracellular protozoan parasites of wild and domestic ruminants transmitted by ixodid ticks]
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Theileria: Intracellular protozoan parasites of wild and domestic ruminants transmitted by ixodid ticks
Ixodid ticks manipulate mammalian host pathways by secreting molecules from salivary glands. Novel cDNAs containing functional secretion signals were isolated from ixodid tick salivary glands using a signal sequence trap. Only 15/61 Rhipicephalus appendiculatus and 1/7 Amblyomma variegatum cDNAs ...
Lambson, B.
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Nene, Vishvanath
,
Obura, M.
,
Shah, Tushaar
,
Pandit, P.
,
Ole-MoiYoi, O.K.
,
Delroux, K.
,
Welburn, S.C.
,
Skilton, Robert A.
,
Villiers, Etienne P. de
,
Bishop, Richard P.
,
[Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells]
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Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells
Bishop, Richard P.
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Sohanpal, B.K.
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Morzaria, S.P.
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Dolan, T.T.
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Mwakima, F.N.
,
Young, A.S.
,
[Discrimination between Theileria parva and Theileria taurotragi in the salivary glands of Rhipicephalus appendiculatus ticks using oligonucleotides homologous to ribosomal RNA sequences]
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Discrimination between Theileria parva and Theileria taurotragi in the salivary glands of Rhipicephalus appendiculatus ticks using oligonucleotides homologous to ribosomal RNA sequences
Tick salivary gland proteins are responsible for suppression/modulation of host immune responses and subsets of such genes whose expression is modified by Theileria parva infection would be candidates for inclusion in anti-tick/anti-pathogen combination vaccines. We used subtractive hybridisation...
Obura, M.
,
Skilton, Robert A.
,
Villiers, Etienne P. de
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Kutima, H.
,
Bishop, Richard P.
,
[Application of suppressive subtractive hybridisation technique to isolate differentially expressed genes in Rhipicephalus appendiculatus ticks following infection by Theileria parva]
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Application of suppressive subtractive hybridisation technique to isolate differentially expressed genes in Rhipicephalus appendiculatus ticks following infection by Theileria parva
Commercial vaccines based on the tick gut protein Bm86 have been successful in controlling the one-host tick Rhipicephalus (Boophilus) microplus and provide heterologous protection against certain other non-target ixodid tick species. This cross protection, however, does not extend to the three-h...
Olds, Cassandra L.
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Mwaura, S.
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Crowder, D.
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Odongo, D.
,
Oers, M.M. van
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Owen, J.
,
Bishop, Richard P.
,
Daubenberger, C.A.
,
[Immunization of cattle with Ra86 impedes Rhipicephalus appendiculatus nymphal-to-adult molting]
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Immunization of cattle with Ra86 impedes Rhipicephalus appendiculatus nymphal-to-adult molting