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Bacteriophage clones containing Theileria parva genomic DNA derived from two different telomeres were isolated and the nucleotide sequences of the telomeric repeats and adjacent telomere-associated (TAS) DNA were determined. The T. parva telomeric repeat sequences, a tandem array of TTTTAGGG or T...


Sohanpal, B.K.Morzaria, S.P.Gobright, E.I.Bishop, Richard P.[Characterisation of the telomeres at opposite ends of a 3 Mb Theileria parva chromosome]Characterisation of the telomeres at opposite ends of a 3 Mb Theileria parva chromosome

Telomere-associated (TA) DNA sequences of the intracellular protozoan parasite Theileria parva were isolated by a novel strategy using a modified version of single-specific-primer polymerase chain reaction (SSP-PCR). Nucleotide sequences of non-coding TA DNA from three telomeres (6017 bp, 2435 bp...


Sohanpal, B.K.Bishop, Richard P.Wasawo, D.[Cloning of telomere-associated DNA using single-specific-primer polymerase chain reaction provides evidence for a conserved sequence directly adjacent to Theileria parva telomeric repeats]Cloning of telomere-associated DNA using single-specific-primer polymerase chain reaction provides evidence for a conserved sequence directly adjacent to Theileria parva telomeric repeats

Protein components of the cement cone of ixodid ticks are candidates for inclusion in vaccines against tick infestation, since they are essential for tick attachment and feeding. We describe here the cloning of a cDNA encoding a 36 kDa protein, designated Rhipicephalus Immuno-dominant Molecule 36...


Bishop, Richard P.Lambson, B.Wells, C.Pandit, P.Osaso, J.Nkonge, C.Morzaria, S.P.Musoke, A.J.Nene, Vishvanath[A cement protein of the tick Rhipicephalus appendiculatus, located in the secretory e cell granules of the type III salivary gland acini, induces strong antibody responses in cattle]A cement protein of the tick Rhipicephalus appendiculatus, located in the secretory e cell granules of the type III salivary gland acini, induces strong antibody responses in cattle

Complete sequencing of p54-gene from 67 European, American, and West and East African Swine Fever virus (ASFV) isolates revealed that West African and European ASFV isolates classified within the predominant Genotype I according to partial sequencing of p72 were discriminated into four major sub-...


Gallardo, C.Mwaengo, D.M.Macharia, J.M.Arias, MarisaTaracha, E.A.Soler Garzón, AlvaroOkoth, S.Martin, E.Kasiti, J.Bishop, Richard P.[Enhanced discrimination of African swine fever virus isolates through nucleotide sequencing of the p54, p72, and pB602L (CVR) genes]Enhanced discrimination of African swine fever virus isolates through nucleotide sequencing of the p54, p72, and pB602L (CVR) genes
Gardner, M.J.Bishop, Richard P.Shah, TushaarVilliers, Etienne P. deCarlton, J.M.Hall, N.Ren, Q.Paulsen, I.T.Pain, A.Berriman, M.Wilson, R.J.M.Sato, S.Ralph, S.A.Mann, D.J.Xiong, Z.Shallom, S.J.Weidman, J.Jiang, L.Lynn,, J.Weaver, B.Shoaibi, A.Domingo, A.R.Wasawo, D.Crabtree, J.Wortman, J.R.Haas, B.Angiuoli, S.V.Creasy, T.H.Lu, C.Suh, B.Silva, J.C.Utterback, T.R.Feldblyum, T.V.Pertea, M.Allen, J.Nierman, W.C.Taracha, E.L.N.Salzberg, S.L.White, O.R.Fitzhugh, H.A.Morzaria, S.P.Venter, J. CraigFraser, C.M.Nene, Vishvanath[Genome sequence of Theileria parva, a bovine pathogen that transforms lymphocytes]Genome sequence of Theileria parva, a bovine pathogen that transforms lymphocytes

Recent studies suggest that peptidyl-prolyl isomerases of the cyclophilin family, that access the secretory pathway, can be involved in the interaction of parasitic protozoa with mammalian host cells. The amino acid sequence of a cDNA encoding a cyclophilin family member of the intracellular pro...


Ebel, T.Pelle, RogerJanoo, R.T.K.Lipp, J.Bishop, Richard P.[A membrane-anchored Theileria parva cyclophilin with a non-cleaved amino-terminal signal peptide for entry into the endoplasmic reticulum]A membrane-anchored Theileria parva cyclophilin with a non-cleaved amino-terminal signal peptide for entry into the endoplasmic reticulum

The nucleotide sequences of the large subunit ribosomal RNA coding genes within the two single copy ribosomal DNA transcription units of a cloned Theileria parva isolate from a buffalo were determined. The two LSU rRNA coding units differed by 11 nucleotide substitutions and two deletions of 1 an...


Bishop, Richard P.Gobright, E.I.Spooner, P.R.Allsopp, B.A.Sohanpal, B.K.Collins, N.[Microsequence heterogeneity and expression of LSU rRNA genes within the two single copy ribosomal transcription units of Theileria parva]Microsequence heterogeneity and expression of LSU rRNA genes within the two single copy ribosomal transcription units of Theileria parva

Ixodid ticks manipulate mammalian host pathways by secreting molecules from salivary glands. Novel cDNAs containing functional secretion signals were isolated from ixodid tick salivary glands using a signal sequence trap. Only 15/61 Rhipicephalus appendiculatus and 1/7 Amblyomma variegatum cDNAs ...


Lambson, B.Nene, VishvanathObura, M.Shah, TushaarPandit, P.Ole-MoiYoi, O.K.Delroux, K.Welburn, S.C.Skilton, Robert A.Villiers, Etienne P. deBishop, Richard P.[Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells]Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells

Molecules located on cell surfaces and those that are secreted from the cell frequently play a critical role in cell biology by mediating interaction with the external environment. These types of parasite molecules often constitute targets for protective host immune responses and are thus of addi...


Musembi, S.Janoo, R.T.K.Sohanpal, B.K.Ochanda, H.Ole-MoiYoi, O.K.Bishop, Richard P.Nene, Vishvanath[Screening for Theileria parva secretory gene products by functional analysis in Saccharomyces cerevisiae]Screening for Theileria parva secretory gene products by functional analysis in Saccharomyces cerevisiae

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