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The nucleotide sequences of the large subunit ribosomal RNA coding genes within the two single copy ribosomal DNA transcription units of a cloned Theileria parva isolate from a buffalo were determined. The two LSU rRNA coding units differed by 11 nucleotide substitutions and two deletions of 1 an...


Bishop, Richard P.Gobright, E.I.Spooner, P.R.Allsopp, B.A.Sohanpal, B.K.Collins, N.[Microsequence heterogeneity and expression of LSU rRNA genes within the two single copy ribosomal transcription units of Theileria parva]Microsequence heterogeneity and expression of LSU rRNA genes within the two single copy ribosomal transcription units of Theileria parva

Ixodid ticks manipulate mammalian host pathways by secreting molecules from salivary glands. Novel cDNAs containing functional secretion signals were isolated from ixodid tick salivary glands using a signal sequence trap. Only 15/61 Rhipicephalus appendiculatus and 1/7 Amblyomma variegatum cDNAs ...


Lambson, B.Nene, VishvanathObura, M.Shah, TushaarPandit, P.Ole-MoiYoi, O.K.Delroux, K.Welburn, S.C.Skilton, Robert A.Villiers, Etienne P. deBishop, Richard P.[Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells]Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells

A cross-sectional survey was carried out in four counties of Jonglei State, South Sudan, between May and June 2012 to determine the distribution and northern limit of Theileria parva, the causative agent of East Coast fever in cattle, and its tick vector Rhipicephalus appendiculatus, as a prerequ...


Marcellino, Wani L.Salih, Diaeldin A.Njahira, Moses N.Ndiwa, Nicholas N.Araba, A.El Hussein, A.M.Seitzer, U.Ahmed, J.S.Bishop, Richard P.Skilton, Robert A.[The Emergence of Theileria parva in Jonglei State, South Sudan: Confirmation using molecular and serological diagnostic tools]The Emergence of Theileria parva in Jonglei State, South Sudan: Confirmation using molecular and serological diagnostic tools
Morzaria, S.P.Spooner, P.R.Bishop, Richard P.Musoke, A.J.Young, J.R.[SfiI and NotI polymorphisms in theileria stocks detected by pulsed field gel electrophoresis]SfiI and NotI polymorphisms in theileria stocks detected by pulsed field gel electrophoresis

Because the function of signal sequences has been conserved during evolution it has been possible to develop both bioinformatics resources to identify them and techniques to clone genes that encode secretary proteins. The latter entail insertion of heterologous signals upstream of signal peptide ...


Nene, VishvanathBishop, Richard P.[Trapping parasite secretory proteins in baker's yeast]Trapping parasite secretory proteins in baker's yeast

Commercial vaccines based on recombinant forms of the Bm86 tick gut antigen are used to control the southern cattle tick, Rhipicephalus microplus, a 1-host species, in Australia and Latin America. We describe herein sequence polymorphism in genes encoding Ra86 homologues of Bm86 in the brown ear ...


Kamau, L.M.Skilton, Robert A.Githaka, Naftaly W.Kiara, Henry K.Kabiru, E.Shah, TushaarMusoke, A.J.Bishop, Richard P.[Extensive polymorphism of Ra86 genes in field populations of Rhipicephalus appendiculatus from Kenya]Extensive polymorphism of Ra86 genes in field populations of Rhipicephalus appendiculatus from Kenya

African swine fever (ASF) has had significant economic and social impact in Nigeria since 1997. However, there has been no effective national response to bring it under control. In this report, we confirm that ASF is still prevalent and widespread in Nigeria. Results from both serosurveillance an...


Fasina, F.O.Shamkai, D.Makinde, A.A.Lombin, L.H.Lazarus, D.D.Rufai, S.A.Adamu, S.S.Agom, D.Pelayo, V.Soler Garzón, AlvaroSimon, A.Adedeji, A.J.Yakubu, M.B.Mantip, S.Benshak, A.J.Okike, I.Anagor, P.Mandeng, D.C.Akanbi, B.O.Ajibade, A.A.Faramade, I.Kazeem, M.M.Enurah, L.U.Bishop, Richard P.Anchuelo, R.Martin, J.H.Gallardo, C.[Surveillance for African Swine fever in Nigeria, 2006-2009]Surveillance for African Swine fever in Nigeria, 2006-2009

A cross-sectional survey was carried out to characterize the pig production systems in four districts along the Kenya-Uganda border. Information was collected by administering structured questionnaires to 645 households in 32 randomly selected clusters. The study showed that the majority of the f...


Nantima, NoelinaOcaido, M.Davies, J.Dione, Michel M.Okoth, Edward A.Mugisha, A.Bishop, Richard P.[Characterization of smallholder pig production systems in four districts along the Uganda-Kenya border]Characterization of smallholder pig production systems in four districts along the Uganda-Kenya border

The preparation of Theileria parva stabilates has been described by Cunningham et al (1973). The method is detailed in the FAO report (1978) and the FAO tick and tick-borne Disease Manual (1984). Theileria parva stabilates are a source of viable sporozoites that can be used to immunise cattle by ...


Morzaria, S.P.Spooner, P.R.Bishop, Richard P.Mwaura, S.[The preparation of a composite stabilate for immunisation against East Coast fever]The preparation of a composite stabilate for immunisation against East Coast fever

There are no commercially available vaccines against human protozoan parasitic diseases, despite the success of vaccination-induced long-term protection against infectious diseases. East Coast fever, caused by the protist Theileria parva, kills one million cattle each year in sub-Saharan Africa, ...


Norling, M.Bishop, Richard P.Pelle, RogerWeihong QiHenson, S.Drábek, E.F.Tretina, K.Odongo, D.Mwaura, S.Njoroge, T.Bongcam-Rudloff, E.Daubenberger, C.A.Silva, J.C.[The genomes of three stocks comprising the most widely utilized live sporozoite Theileria parva vaccine exhibit very different degrees and patterns of sequence divergence]The genomes of three stocks comprising the most widely utilized live sporozoite Theileria parva vaccine exhibit very different degrees and patterns of sequence divergence

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