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The extent of 5S and 45S ribosomal DNA (rDNA) variation was investigated in wild and domesticated common beans (Phaseolus vulgaris) chosen to represent the known genetic diversity of the species. 5S and 45S rDNA probes were localized on mitotic chromosomes of 37 accessions by fluorescent in situ ...


Pedrosa Harand, ASouza Almeida, C.C. deMosiolek, MBlair, Matthew W.Schweizer, DGuerra, M[Extensive ribosomal DNA amplification during Andean common bean (Phaseolus vulgaris L.) evolution]Extensive ribosomal DNA amplification during Andean common bean (Phaseolus vulgaris L.) evolution

Four multiplex panels of fluorescent-labeled rice microsatellite markers were designed to survey allelic diversity at a total of 27 simple sequence repeat loci. Automated fragment detection, size calling and binning were used to identify the microsatellite alleles. The panels were tested on 72 ac...


Blair, Matthew W.Hedetale, VMcCouch, Susan R.[Fluorescent-labeled microsatellite panels useful for detecting allelic diversity in cultivated rice (Oryza sativa L.)]Fluorescent-labeled microsatellite panels useful for detecting allelic diversity in cultivated rice (Oryza sativa L.)

Background: Gene-based (genic) microsatellites are a useful tool for plant genetics and simple sequence repeat loci can often be found in coding regions of the genome. While EST sequencing can be used to discover genic microsatellites, direct screening of cDNA libraries for repeat motifs can save...


Blair, Matthew W.Muñoz Torres, M.C.Giraldo, M.C.Pedraza, F[Development and diversity of Andean-derived, gene-based microsatellites for common bean (Phaseolus vulgaris L.)]Development and diversity of Andean-derived, gene-based microsatellites for common bean (Phaseolus vulgaris L.)

Common bean (Phaseolus vulgaris) is the major food legume worldwide, making it an important target for novel approaches of genetic analysis. This study evaluated the use of ethyl methane sulfonate (EMS) for the generation of a mutant population for targeted induced local lesions in genomes (TILLI...


Porch, TGBlair, Matthew W.Lariguet, PGaleano Mendoza, CH[Generation of a mutant population for TILLING common bean genotype BAT 93]Generation of a mutant population for TILLING common bean genotype BAT 93

Common bean can be grown as a grain crop (dry beans) or as a fresh vegetable (snap beans/green beans), both items being important in nutritional terms for providing essential minerals and vitamins to the diet. Snap beans are thought to be derived predominantly from dry beans of the Andean genepoo...


Blair, Matthew W.Chaves, A.Tofiño Rivera, Adriana PatriciaPalacio Mejia, JDCalderón Sotelo, J.F.[Extensive diversity and inter-genepool introgression in a world-wide collection of indeterminate snap bean accessions]Extensive diversity and inter-genepool introgression in a world-wide collection of indeterminate snap bean accessions

Cultivated common bean germplasm is especially diverse due to the parallel domestication of two genepools in the Mesoamerican and Andean centers of diversity and introgression between these gene pools. Classification into morphological races has helped to provide a framework for utilization of th...


Blair, Matthew W.Díaz, Lucy MilenaBuendía Castellanos, Hector F.Duque E., Myriam Cristina[Genetic diversity, seed size associations and population structure of a core collection of common beans (Phaseolus vulgaris L.)]Genetic diversity, seed size associations and population structure of a core collection of common beans (Phaseolus vulgaris L.)

The present study describes a new set of 61 polymorphic microsatellite markers for beans and the construction of a genetic map using the BAT93 x Jalo EEP558 (BJ) population for the purpose of developing a reference linkage map for common bean (Phaseolus vulgaris). The main objectives were to inte...


Grisi, MCMBlair, Matthew W.Gepts, Paul L.Brondani, C.Pereira, PAABrondani, Rosana Pereira V.[Genetic mapping of a new set of microsatellite markers in a reference common bean (Phaseolus vulgaris) population BAT93 x Jalo EEP558]Genetic mapping of a new set of microsatellite markers in a reference common bean (Phaseolus vulgaris) population BAT93 x Jalo EEP558

Phytate is an important antinutritional component of legume seeds, which chelates minerals that are essential to the human diet such as iron and zinc. Phytate levels are often correlated with total seed phosphorus (P). The objective of this research was to evaluate quantitative trait loci (QTL) f...


Blair, Matthew W.Sandoval Medina, TACaldas, GVBeebe, Stephen E.Paez, MI[Quantitative trait locus analysis of seed phosphorus and seed phytate content in a recombinant inbred line population of common bean]Quantitative trait locus analysis of seed phosphorus and seed phytate content in a recombinant inbred line population of common bean
Blair, Matthew W.Restrepo, J.Pradilla, A.Gracia, BAraújo, C.Plata, C. deMosquera, M.Ariza Nieto, MGlahn, Raymond P.Pachón, Helena[Development and utilization of high mineral beans in combination with quality protein maize as a nutritional intervention in Southwestern Colombia]Development and utilization of high mineral beans in combination with quality protein maize as a nutritional intervention in Southwestern Colombia

Background: Common bean (Phaseolus vulgaris L.) is the most important legume for direct human consumption and the goal of this study was to integrate a recently constructed physical map for the species with a microsatellite based genetic map using a BAC library from the genotype G19833 and the re...


Córdoba, J.M.Chavarro, CSchlueter, JAJackson, S.A.Blair, Matthew W.[Integration of physical and genetic maps of common bean through BAC-derived microsatellite markers]Integration of physical and genetic maps of common bean through BAC-derived microsatellite markers

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