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A modified NC membrane-based dot-ELISA was used to detect and differentiate between Trypanosoma brucei, T. congolense and T. simiae procyclics in the midguts of experimentally infected tsetse flies. The modification of the assay consisted of (a) the lysis of T. congolense or T. simiae in NC membr...


Bosompem, K.M.Moloo, S.K.Assoku, R.K.G.Nantulya, V.M.[Detection and differentiation between trypanosome species in experimentally infected tsetse flies (Glossina spp.) using dot-ELISA]Detection and differentiation between trypanosome species in experimentally infected tsetse flies (Glossina spp.) using dot-ELISA

Diagnosis of trypanosome infections in tsetse (Glossina spp.) is currently based on dissection and microscopic examination of different organs of the vector for the presence of infecting trypanosomes. This procedure is slow and labour intensive, and it is not accurate. The work reported in this t...


Bosompem, K.M.[Development of monoclonal antibody-based assays for detection and differentiation of trypanosome species in the tsetse fly (Glossina SPP).]Development of monoclonal antibody-based assays for detection and differentiation of trypanosome species in the tsetse fly (Glossina SPP).

A rapid, visually read, dot-ELISA developed for the detection and differentiation of trypanosome species in tsetse flies (Glossina spp.), was field tested alongside the standard fly dissection method on a ranch in south eastern Kenya. Of 104 G. pallidipes dissected, 2 were found to be infected wi...


Bosompem, K.M.Masake, R.A.Assoku, R.K.G.Opiyo, E.A.Nantulya, V.M.[Field evaluation of a dot-ELISA for the detection and differentiation of trypanosome species in infected tsetse flies (Glossina spp.)]Field evaluation of a dot-ELISA for the detection and differentiation of trypanosome species in infected tsetse flies (Glossina spp.)

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