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The cassava core collection was selected to represent, with minimum repetitiveness, the potential genetic diversity of the crop. The core (630 accessions) was chosen from the base collection (over 5500 accessions) on the basis of diversity of origin (country and geographic), morphology, isozyme p...


Chavarriaga Aguirre, PaulMaya, M.M.Tohme, Joseph M.Duque E., Myriam CristinaIglesias, C.A.Bonierbale, Merideth W.Kresovich, SKochert, G[Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-BASED markers to maintain germplasm collections]Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-BASED markers to maintain germplasm collections

Restriction fragment analysis was used to determine the variation of chloroplast DNA (cpDNA) among and within different species of Passiflora. Total DNAs of 35 accessions, representing 12 species, were digested with four restriction endonucleases. Southern blots of the restriction digests were th...


Sánchez, I.Angel, F.Grum, M.Duque E., Myriam CristinaLobo Arias, MTohme, Joseph M.Roca, William M.[Variability of chloroplast DNA in the genus Passiflora L.]Variability of chloroplast DNA in the genus Passiflora L.

Se utilizó la información generada por 29 microsatélites en 63 accesiones del acervo andino de P. vulgaris para elaborar agrupamientos y calcular índices de prevalencia. Las accesiones formaron grupos que correspondieron a las razas Nueva Granada, Chile y Perú. Se determinó la existencia de notor...


Blair, Matthew W.Díaz, J.M.Duque E., Myriam CristinaHidalgo, R.[Evidencia molecular de diferenciación genética e introgresión entre razas de frijol común del acervo andino = Molecular evidence for race structure and introgression among Andean races of common bean]Evidencia molecular de diferenciación genética e introgresión entre razas de frijol común del acervo andino = Molecular evidence for race structure and introgression among Andean races of common bean

The genetic structure of rice blast pathogen populations was studied using 149 and 107 monoconidial isolates from Los Palacios and Sur del Jíbaro rice production locations in the western and central regions of Cuba, respectively. These isolates were collected from 35 rice genotypes and total DNA ...


Fuentes, JLCorrea Victoria, Fernando J.Escobar, F.Mora, LDuque E., Myriam CristinaDeus, J.E.Cornide, MT[Genetic diversity analysis of the rice blast pathogen population of two locations in Cuba]Genetic diversity analysis of the rice blast pathogen population of two locations in Cuba

Restriction fragment length polymorphisms (RFLPs) were used to study the population genetics and temporal dynamics of the cassava bacterial pathogen Xanthomonas axonopodis pv. manihotis. The population dynamics were addressed by comparing samples collected from 1995 to 1999 from six locations, sp...


Restrepo, S.Velez, C.M.Duque E., Myriam CristinaVerdier, Valérie[Genetic structure and population dynamics of Xanthomonas axonopodis pv. manihotis in Colombia from 1995 to 1999]Genetic structure and population dynamics of Xanthomonas axonopodis pv. manihotis in Colombia from 1995 to 1999

Cassava bacterial blight (CBB), caused by Xanthomonas axonopodis pv. manihotis (Xam), is a major disease of cassava (Manihot esculenta Crantz) in Africa and South America. Planting resistant varieties is the preferred method of disease control. Recent genetic mapping of an F1 cross (TMS 30572 × C...


Jorge, VFregene, Martin A.Duque E., Myriam CristinaBonierbale, Merideth W.Tohme, Joseph M.Verdier, Valérie[Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz)]Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz)

Microsatellite polymorphism was studied in a sample of 39 traditional rice (Oryza sativa L.) varieties and 11 improved varieties widely planted in Cuba. The study was aimed at assessing the extent of genetic variation in traditional and improved varieties and to establish their genetic relationsh...


Álvarez, A.Fuentes, JLPuldon, VGómez, P.J.Mora, LDuque E., Myriam CristinaGallego Sánchez, Gerardo J.Tohme, Joseph M.[Genetic diversity analysis of Cuban traditional rice (Oryza sativa L.) varieties based on microsatellite markers]Genetic diversity analysis of Cuban traditional rice (Oryza sativa L.) varieties based on microsatellite markers

The tropical multipurpose shrub legume Cratylia argentea is well adapted to acid soils of low to medium fertility and has excellent drought-tolerance. Due to its high nutritive value it is particularly suited as forage for dry-season supplementation. A collection of 47 C. argentea accessions in a...


Andersson, Meike S.Schultze-Kraft, RainerPeters, MichaelDuque E., Myriam CristinaGallego Sánchez, Gerardo J.[Extent and structure of genetic diversity in a collection of the tropical multipurpose shrub legume Cratylia argentea (Desv.) O. kuntze as revealed by RAPD markers]Extent and structure of genetic diversity in a collection of the tropical multipurpose shrub legume Cratylia argentea (Desv.) O. kuntze as revealed by RAPD markers

Cultivated common bean germplasm is especially diverse due to the parallel domestication of two genepools in the Mesoamerican and Andean centers of diversity and introgression between these gene pools. Classification into morphological races has helped to provide a framework for utilization of th...


Blair, Matthew W.Díaz, Lucy MilenaBuendía Castellanos, Hector F.Duque E., Myriam Cristina[Genetic diversity, seed size associations and population structure of a core collection of common beans (Phaseolus vulgaris L.)]Genetic diversity, seed size associations and population structure of a core collection of common beans (Phaseolus vulgaris L.)

Several molecular marker systems have been developed for assessing genetic diversity in crop germplasm collections. A trade-off often exists between the number of loci that can feasibly be sampled by a marker system and the amount of information provided by each locus. We compared the usefulness ...


Hurtado Lopez, PXOlsen, KMBuitrago, C.Ospina, C.Marin, JADuque E., Myriam CristinaVicente, M.C. deWongtiem, PrapitWenzl, PeterKillian, AAdekele, M.Fregene, Martin A.[Comparison of simple sequence repeat (SSR) and diversity array technology (DArT) markers for assessing genetic diversity in cassava Manihot esculenta Crantz)]Comparison of simple sequence repeat (SSR) and diversity array technology (DArT) markers for assessing genetic diversity in cassava Manihot esculenta Crantz)

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