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Escobar Pérez, Roosevelt H.Manrique Carpintero, Norma ConstanzaRíos, AMuñoz Flórez, Ligia CarmenzaMafla, G.Debouck, Daniel G.Tohme, Joseph M.[Implementation of the encapsulation-dehydration cryopreservation method for the cassava core collection]Implementation of the encapsulation-dehydration cryopreservation method for the cassava core collection

Shoot tips of in vitro-grown plantlets of cassava (Manihot esculenta Crantz), representing a wide range of germplasm, were cryopreserved as follows: precultured for 3 days, cryoprotected and dehydrated for 1 h, then frozen in liquid nitrogen using a six-step protocol. After 3 h in liquid nitrogen...


Escobar Pérez, Roosevelt H.Mafla, G.Roca, William M.[A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen]A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen
Escobar Pérez, Roosevelt H.Manrique Carpintero, Norma ConstanzaGil V, FTohme, Joseph M.Debouck, Daniel G.[Preliminary studies on the cryopreservation of meristems and seeds of wild Manihot species]Preliminary studies on the cryopreservation of meristems and seeds of wild Manihot species

Cassava is mostly growing by resource-limited farmers in the Americas, Africa, Asia and Oceania. The crop is an important source of food and income for millions in the tropics. Vegetative propagation, bulkiness of planting material and risk of genetic erosion make cassava an ideal target for inno...


Escobar Pérez, Roosevelt H.Mafla, G.Roca, William M.[Cryopreservation for long-term conservation of cassava genetic resources]Cryopreservation for long-term conservation of cassava genetic resources

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