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Journal Article 2001

Autor: Wambugu, J. Kanguha, E. Hanotte, Olivier H. Davis, S. Taylor, J. Skow, L. Brunner, R. Schwerin, M. Teale, A.J. Iraqi, F.A.

Two bovine bacterial artificial chromosome clones were screened by PCR to amplify the tumour necrosis factor alpha (TNFA) gene. DNA extraction and Southern blotting and probing with a (GT)10 oligonucleotide were performed, followed by cloning into the pUC19 vector. A 0.9 kb fragment containing se...

Enlace original: https://cgspace.cgiar.org/handle/10568/28253

Investigación en Agricultura
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Wambugu, J. ,  Kanguha, E. ,  Hanotte, Olivier H. ,  Davis, S. ,  Taylor, J. ,  Skow, L. ,  Brunner, R. ,  Schwerin, M. ,  Teale, A.J. ,  Iraqi, F.A. ,  [Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone] ,  Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone
Journal Article 2000

Autor: Schmidt, P. Kuhn, C. Kang'a, S. Hanotte, Olivier H. Vanselow, J. Anton, I. Langner, Ch Schwerin, M.

The paper first describes interleukin-12 P35 encoding gene and discusses its role. It then looks into PCR conditions and sequencing; genotyping; sequencing; polymorphism frequency; mendelian inheritance; chromosomal location in sheep and cattle of different species.

Enlace original: https://cgspace.cgiar.org/handle/10568/30069

Investigación en Agricultura
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Schmidt, P. ,  Kuhn, C. ,  Kang'a, S. ,  Hanotte, Olivier H. ,  Vanselow, J. ,  Anton, I. ,  Langner, Ch ,  Schwerin, M. ,  [Interleukin - 12 p35 encoding gene of cattle and sheep harbours a polymorphic T stretch in intron 4] ,  Interleukin - 12 p35 encoding gene of cattle and sheep harbours a polymorphic T stretch in intron 4
Journal Article 2003

Autor: Nijman, I.J. Otsen, M. Verkaar, E.L.C. Ruijter, C. de Hanekamp, E. Ochieng, J.W. Shamshad, S. Rege, J.E.O. Hanotte, Olivier H. Barwegen, M.W. Sulawati, T. Lenstra, J.A.

Hybridization between wild and domestic bovine species occurs worldwide either spontaneously or by organized crossing. We have analysed hybridization of banteng (Bos javanicus) and zebu (Bos indicus) in south-east Asian cattle using mitochondrial DNA (PCR-RFLP and sequencing), AFLP, satellite fr...

Enlace original: https://cgspace.cgiar.org/handle/10568/32933

Investigación en Agricultura
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Nijman, I.J. ,  Otsen, M. ,  Verkaar, E.L.C. ,  Ruijter, C. de ,  Hanekamp, E. ,  Ochieng, J.W. ,  Shamshad, S. ,  Rege, J.E.O. ,  Hanotte, Olivier H. ,  Barwegen, M.W. ,  Sulawati, T. ,  Lenstra, J.A. ,  [Hybridization of Banteng (Bos javanicus) and Zebu (Bos indicus) revealed by mitochondrial DNA, satellite DNA, AFLP and microsatellites] ,  Hybridization of Banteng (Bos javanicus) and Zebu (Bos indicus) revealed by mitochondrial DNA, satellite DNA, AFLP and microsatellites
Journal Article 1996

Autor: Lowe, A.J. Hanotte, Olivier H. Guarino, Luigi

Random amplified polymorphic DNA (RAPD) variation is being used increasingly to characterize germplasm collections. However, the technique has limitations due to the lack of reproducibility of results under certain conditions. Here we review some of the literature on the factors that are mainly r...

Enlace original: https://cgspace.cgiar.org/handle/10568/29494

Investigación en Agricultura
DNA
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Lowe, A.J. ,  Hanotte, Olivier H. ,  Guarino, Luigi ,  [Standardization of molecular genetic techniques for the characterization of germplasm collections: The case of random amplified polymorphic DNA (RAPD)] ,  Standardization of molecular genetic techniques for the characterization of germplasm collections: The case of random amplified polymorphic DNA (RAPD)
Journal Article 2000

Autor: Han Jianlin Mburu, D. Ochieng, J. Kaufmann, J.B. Rege, O. Hanotte, Olivier H.

Twenty new world camelidae primer pairs were selected for cross-species amplification of loci in the old world camelids, the dromedary (Camelus dromedarius) and the bacterian (camelus bactrianus). A panel of DNA samples from 34 unrelated dromedaries (Kenya) and from 34 unrelated bactrians (32 dom...

Enlace original: https://cgspace.cgiar.org/handle/10568/30070

Investigación en Agricultura
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Han Jianlin ,  Mburu, D. ,  Ochieng, J. ,  Kaufmann, J.B. ,  Rege, O. ,  Hanotte, Olivier H. ,  [Application of new world camelidae microsatellite primers for amplification of polymorphic loci in old world camelids] ,  Application of new world camelidae microsatellite primers for amplification of polymorphic loci in old world camelids
Journal Article 1997

Autor: Hanotte, Olivier H. Okomo, M. Verjee, Y. Rege, J.E.O. Teale, A.J.

DNA samples from 30 Friesian, 18 N'Dama, 30 Sahiwal and 18 Barotse (derived from Bos taurus and B. indicus) cattle were amplified by the polymerase chain reaction using previously described primer sequences for amplification of the Y chromosome locus INRA124. A polymorphism with 2 alleles was det...

Enlace original: https://cgspace.cgiar.org/handle/10568/28251

Investigación en Agricultura
Enlace permanente
Hanotte, Olivier H. ,  Okomo, M. ,  Verjee, Y. ,  Rege, J.E.O. ,  Teale, A.J. ,  [A polymorphic Y chromosome microsatellite locus in cattle] ,  A polymorphic Y chromosome microsatellite locus in cattle
Journal Article 2000

Autor: Wambugu, J. Kanguha, E. Hanotte, Olivier H. Davis, S. Taylor, J. Skow, L. Teale, A.J. Iraqi, F.A.
Enlace original: https://cgspace.cgiar.org/handle/10568/30068

Investigación en Agricultura
Enlace permanente
Wambugu, J. ,  Kanguha, E. ,  Hanotte, Olivier H. ,  Davis, S. ,  Taylor, J. ,  Skow, L. ,  Teale, A.J. ,  Iraqi, F.A. ,  [Identification of a polymorphic dinucleotide repeat on a bovine TNF-alpha containing BAC clone] ,  Identification of a polymorphic dinucleotide repeat on a bovine TNF-alpha containing BAC clone

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