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Eisler, M.C.Arowolo, R.O.A.Gault, E.A.Moloo, S.K.Holmes, P.H.Peregrine, A.S.[Isometamidium concentrations in the sera of Boran cattle. Correlation with prophylaxis against tsetse-transmitted Trypanosoma congolense]Isometamidium concentrations in the sera of Boran cattle. Correlation with prophylaxis against tsetse-transmitted Trypanosoma congolense

A survey to investigate resistance to drugs used in the treatment of bovine trypanosomosis was conducted in the eastern province of Zambia between 1996 and 1998. A cross-sectional study was conducted in three districts (Petauke, Katete, Lundazi) at 34 village sampling sites selected at random fr...


Sinyangwe, L.Delespaux, V.Brandt, J.Geerts, S.Mubanga, J.Machila, N.Holmes, P.H.Eisler, M.C.[Trypanocidal drug resistance in eastern province of Zambia]Trypanocidal drug resistance in eastern province of Zambia

The relationship between serum concentrations of the prophylactic trypanocidal drug isometamidium chloride and protection against tsetse challenge with two populations of Trypanosoma congolense was investigated in Boran (Bos indicus) cattle, using an isometamidium-ELISA. Isometamidium chloride (S...


Eisler, M.C.Gault, E.A.Moloo, S.K.Holmes, P.H.Peregrine, A.S.[Concentrations of isometamidium in the sera of cattle challenged with drug-resistant Trypanosoma congolense]Concentrations of isometamidium in the sera of cattle challenged with drug-resistant Trypanosoma congolense
Whitelaw, D.D.Bell, I.R.Holmes, P.H.Moloo, S.K.Hirumi, H.Urquhart, G.M.Murray, M.[Isometamidium chloride prophylaxis against Trypanosoma congolense challenge and the development of immune responses in Boran cattle]Isometamidium chloride prophylaxis against Trypanosoma congolense challenge and the development of immune responses in Boran cattle

Control of trypanosomes in cattle, sheep and goats in endemic areas has depended largely on the use of chemotherapeutic or chemoprophylactic agents. One such agent is homidium. The normal use of homidium has been in the treatment of infections due to both Trypanosoma congolense and T.vivax in cat...


Murilla, G.A.Peregrine, A.S.Holmes, P.H.Eisler, M.C.Ndung'u, J.M.[Investigation into the effects of Trypanosoma congolense infections on the pharmacokinetics of Homidium in Boran cattle]Investigation into the effects of Trypanosoma congolense infections on the pharmacokinetics of Homidium in Boran cattle

Resistance to the drugs used to control African animal trypanosomosis is increasingly recognised as a constraint to livestock production in sub-Saharan Africa. The most commonly used tests for detection of trypanocidal drug resistance are tests using mice or ruminants, but these suffer from lack ...


Eisler, M.C.Brandt, J.Bauer, B.Clausen, P.H.Delespaux, V.Holmes, P.H.Ilemobade, A.Machila, N.Mbwambo, H.McDermott, John J.Mehlitz, D.Murilla, G.Ndung'u, J.M.Peregrine, A.S.Sidibe, I.Sinyangwe, L.Geerts, S.[Standardised tests in mice and cattle for the detection of drug resistance in tsetse-transmitted trypanosomes of African domestic cattle]Standardised tests in mice and cattle for the detection of drug resistance in tsetse-transmitted trypanosomes of African domestic cattle

Two groups of five Boran (Bos indicus) cattle were infected with one of two populations of Trypanosoma congolense; one drug-sensitive (IL1180), and one drug-resistant (IL3330). The animals were then treated intramuscularly with homidium bromide at a dose rate of 1.0 mg kg - bodyweight 7 days afte...


Murilla, G.A.Peregrine, A.S.Ndung'u, J.M.Holmes, P.H.Eisler, M.C.[The effects of drug-sensitive and drug-resistant Trypanosoma congolense infections on the pharmacokinetics of homidium in Boran cattle]The effects of drug-sensitive and drug-resistant Trypanosoma congolense infections on the pharmacokinetics of homidium in Boran cattle

Two enzyme-linked immunosorbent assays (ELISA) for the determination of homidium in serum of treated cattle have been developed and evaluated. One is a direct competition (Assay 1) and the other an indirect competition assay (Assay 2). Both assays are highly sensitive with a limit of detection of...


Murilla, G.Eisler, M.C.Peregrine, A.S.Ndung'u, J.M.Holmes, P.H.[Development and evaluation of an enzyme-linked immunosorbent assay (ELISA) for the determination of the trypanocidal drug homidium in serum of treated cattle]Development and evaluation of an enzyme-linked immunosorbent assay (ELISA) for the determination of the trypanocidal drug homidium in serum of treated cattle

Pharmacokinetic studies on the trypanocidal drug homidium bromide using a competitive enzyme immunoassay (detection limit 0.1 ng/mL) are reported for non-infected Friesian and Boran steers following treatment with homidium bromide at a dose of 1.0 mg/kg b.w. Following intravenous (i.v.) treatment...


Murilla, G.Holmes, P.H.Peregrine, A.S.Eisler, M.C.Ndung'u, J.M.[Some pharmacokinetic parameters of the trypanocidal drug homidium bromide in Friesian and Boran steers using an enzyme-linked immunosorbent assay (ELISA)]Some pharmacokinetic parameters of the trypanocidal drug homidium bromide in Friesian and Boran steers using an enzyme-linked immunosorbent assay (ELISA)

Control of trypanosomosis in cattle, sheep and goats in endemic areas has depended largely on the use of chemotherapeutic or chemoprophylactic agents. One such agent is homidium. The normal use of homidium has been in the treatment of infections due to both Trypanosoma congolense and T. vivax in ...


Murilla, G.Peregrine, A.S.Holmes, P.H.Eisler, M.C.Ndung'u, J.M.[Investigation into the effects of Trypanosoma congolense infections on the pharmacokinetics of Homidium in Boran cattle]Investigation into the effects of Trypanosoma congolense infections on the pharmacokinetics of Homidium in Boran cattle

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