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Heartwater, a tick-borne disease of domestic and wild ruminants, is caused by the intracellular rickettsia Ehrlichia ruminantium (previously known as Cowdria ruminantium). It is a major constraint to livestock production throughout subSaharan Africa, and it threatens to invade the Americas, yet t...


Collins, N.E.Liebenberg, J.Villiers, Etienne P. deBrayton, K.A.Louw, E.Pretorius, E.Faber, F.E.Heerden, H. vanJosemans, A.Kleef, M. vanSteyn, H.C.Strijp, M.F. vanZweygarth, E.Jongejan, F.Maillard, J.C.Berthier, D.Botha, M.Joubert, F.Corton, C.H.Thomson, N.R.Allsopp, M.T.Allsopp, B.A.[The genome of the heartwater agent Ehrlichia ruminantium contains multiple tandem repeats of actively variable copy number]The genome of the heartwater agent Ehrlichia ruminantium contains multiple tandem repeats of actively variable copy number

We have developed two loop-mediated isothermal amplification (LAMP) assays for the detection of Theileria parva, the causative agent of East Coast fever (ECF), an economically important cattle disease in eastern, central and southern Africa. These assays target the polymorphic immunodominant mole...


Thekisoe, O.M.M.Rambritch, N.E.Nakao, R.Bazie, R.S.Mbati, P.Namangala, B.Malele, I.Skilton, Robert A.Jongejan, F.Sugimoto, C.Kawazu, S.I.Inoue, N.[Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes]Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes

Heartwater, a tick-borne disease of domestic and wild ruminants, is caused by the intracellular rickettsia Ehrlichia ruminantium (previously known as Cowdria ruminantium). It is a major constraint to livestock production throughout subSaharan Africa, and it threatens to invade the Americas, yet t...


Collins, N.E.Liebenberg, J.Villiers, Etienne P. deBrayton, K.A.Louw, E.Pretorius, A.Faber, F.E.Heerden, H. vanJosemans, A.Kleef, M. vanSteyn, H.C.Strijp, M.F. vanZweygarth, E.Jongejan, F.Maillard, J.C.Berthier, D.Botha, M.Joubert, F.Corton, C.H.Thomson, N.R.Allsopp, M.T.Allsopp, B.A.[The genome of the heartwater agent Ehrlichia ruminantium contains multiple tandem repeats of actively variable copy number]The genome of the heartwater agent Ehrlichia ruminantium contains multiple tandem repeats of actively variable copy number

The response of peripheral blood mononuclear cells (PBMC) collected from cattle immunized with killed C. ruminantium to MAP1, MAP1-B, MAP2 and to a lysate of Cowdria was characterized in vitro. CD4+ T lymphocytes responded strongly to Cowdria lysates while B cells and gamma delta T cells prolifer...


Totte, P.McKeever, Declan J.Jongejan, F.Barbet, A.Mahan, S.M.Mwangi, D.Bensaid, A.[Analysis of cellular responses to native and recombinant proteins of Cowdria ruminantium]Analysis of cellular responses to native and recombinant proteins of Cowdria ruminantium

The major sporozoite surface antigen of Theileria annulata (SPAG-1) is a candidate for inclusion in a subunit vaccine. In this paper we summarize the results of 4 vaccination experiments using recombinant SPAG-1 expressed in different systems and presented in different adjuvants. The antigen has ...


Boulter, N.Brown, D.Wilkie, G.Williamson, S.Kirvar, E.Knight, P.Glass, E.Campbell, J.Morzaria, S.P.Nene, VishvanathMusoke, A.J.Oliveria, C. deGubbels, M.J.Jongejan, F.Hall, R.[Evaluation of recombinant sporozoite antigen SPAG-1 as a vaccine candidate against Theileria annulata by the use of different delivery systems]Evaluation of recombinant sporozoite antigen SPAG-1 as a vaccine candidate against Theileria annulata by the use of different delivery systems

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