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A preliminary search for QTLs in a cattle F2 resource populations segregating for trypanotolerance has revealed three large candidate chromosomal regions (Bta02, Bta05, Bta07). In order to narrow down the position of the trypanotolerant QTLs, we are building comparative chromosomal maps between c...


Nilsson, P.H.Kang'a, S.Rottengatter, K.Suedbeck, U.Iraqui, F.Mwakaya, J.Mwangi, D.Womack, J.E.Goldammer, T.Schwerin, M.Bradley, D.Agaba, MorrisSugimoto, K.Gelhaus, A.Horstmann, R.Teale, A.J.Kemp, Stephen J.Hanotte, Olivier H.[Radiation hybrid maps of candidate trypanotolerance regions in cattle]Radiation hybrid maps of candidate trypanotolerance regions in cattle
Autor: Kang'a, S.

Tsetse-transmitted trypanosomosis poses severe constraints to livestock productivity in sub-Saharan Africa, affecting large areas of the continent that hold the greatest potential for agricultural development. Greater exploitation of the genetic resistance to trypanosomosis (trypananotolerance) t...


Kang'a, S.[Development and application of genetic markers linked to A QTL controlling resistance to trypanosomosis on bovine chromosome 7.]Development and application of genetic markers linked to A QTL controlling resistance to trypanosomosis on bovine chromosome 7.

We describe the generation of an auto-annotated index of genes that are expressed in the salivary glands of four-day fed female adult Rhipicephalus appendiculatus ticks. A total of 9162 EST sequences were derived from an uninfected tick cDNA library and 9844 ESTs were from a cDNA library from tic...


Nene, VishvanathLee, D.Kang'a, S.Skilton, Robert A.Shah, TushaarVilliers, Etienne P. deMwaura, S.Taylor, D.Quackenbush, J.Bishop, Richard P.[Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva]Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva

An alternative approach for the direct analysis of chromosome regions corresponding to economical traits, based on chromosome microdissection, is described. Large fragment clones isolated with primer pairs designed from chromosome fragment-specific DNA sequences were localized by fluorescence in ...


Goldammer, T.Brunner, R.M.Kang'a, S.Hanotte, Olivier H.Schwerin, M.[Establishment of bovine chromosome region specific high resolution maps using microdissection]Establishment of bovine chromosome region specific high resolution maps using microdissection

We describe the generation of an auto-annotated index of genes that are expressed in the salivary glands of four-day fed female adult Rhipicephalus appendiculatus ticks. A total of 9162 EST sequences were derived from an uninfected tick cDNA library and 9844 ESTs were from a cDNA library from t...


Nene, VishvanathLee, D.Kang'a, S.Skilton, Robert A.Shah, TushaarVilliers, Etienne P. deMwaura, S.Taylor, D.Quackenbush, J.Bishop, Richard P.[Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva]Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva

The paper first describes interleukin-12 P35 encoding gene and discusses its role. It then looks into PCR conditions and sequencing; genotyping; sequencing; polymorphism frequency; mendelian inheritance; chromosomal location in sheep and cattle of different species.


Schmidt, P.Kuhn, C.Kang'a, S.Hanotte, Olivier H.Vanselow, J.Anton, I.Langner, ChSchwerin, M.[Interleukin - 12 p35 encoding gene of cattle and sheep harbours a polymorphic T stretch in intron 4]Interleukin - 12 p35 encoding gene of cattle and sheep harbours a polymorphic T stretch in intron 4

The intracellular protozoan parasite Theileria parva transforms bovine lymphocytes inducing uncontrolled proliferation. Proteins released from the parasite are assumed to contribute to phenotypic changes of the host cell and parasite persistence. With 85 members, genes encoding subtelomeric varia...


Schmuckli-Maurer, J.Casanova, C.S.Affentranger, S.Kang'a, S.Nene, VishvanathKatzer, F.McKeever, Declan J.Müller, JoachimBishop, Richard P.Arnab Pain, A.Dobbelaere, D.A.E.[Expression analysis of the Theileria parva Subtelomere-encoded Variable Secreted Protein gene family]Expression analysis of the Theileria parva Subtelomere-encoded Variable Secreted Protein gene family
Kang'a, S.Nilsson, P.Goldammer, T.Mwakaya, J.Njagi, E.N.M.Schwerin, M.Hanotte, Olivier H.[Construction of a BAC contig at a cattle QTL region controlling resistance to trypanosomosis on BTA7 that is homologous to the murine T1R1 region on MMU17]Construction of a BAC contig at a cattle QTL region controlling resistance to trypanosomosis on BTA7 that is homologous to the murine T1R1 region on MMU17
Kang'a, S.Nilsson, P.Goldammer, T.Mwakaya, J.Elliot, J.S.Womack, J.E.Schwerin, M.Njagi, E.N.M.Hanotte, Olivier H.[From QTL to candidate genes - an example from QTL mapping for trypanosomiasis resistance in cattle. Abstract]From QTL to candidate genes - an example from QTL mapping for trypanosomiasis resistance in cattle. Abstract

Trypanosomiasis is one of the most important diseases in African cattle. About 3 million cattle die every year by this disease. However, about 5 percent of the total African cattle have a natural resistance against trypanosomiasis. In a preliminary study markers linked to trypanotolerance have be...


Goldammer, T.Brunner, R.M.Kang'a, S.Hanotte, Olivier H.Schwerin, M.[Generation of a bovine BAC pool for chromosome region BTA7q14-22 correlated to the trait trypanotolerance]Generation of a bovine BAC pool for chromosome region BTA7q14-22 correlated to the trait trypanotolerance

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