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Paling, R.W.Leak, S.G.A.Katende, J.Kamunya, G.W.Moloo, S.K.[Epidemiology of animal trypanosomiasis on a cattle ranch in Kilifi, Kenya]Epidemiology of animal trypanosomiasis on a cattle ranch in Kilifi, Kenya
Shompole, S.McElwain, T.F.Jasmer, D.P.Hines, S.A.Katende, J.Musoke, A.J.Rurangirwa, F.R.McGuire, T.C.[Identification of Babesia bigemina infected erythrocyte surface antigens containing epitopes conserved among strains]Identification of Babesia bigemina infected erythrocyte surface antigens containing epitopes conserved among strains

Tick-borne diseases (TBDs) are a major economic constraint to livestock production in sub-Saharan Africa. ILRI is focussing on developing a range of products, such as vaccines, diagnostics and decision support services to underpin improved control programmes against these diseases. We have develo...


Morzaria, S.P.Katende, J.Musoke, A.J.Nene, VishvanathSkilton, Robert A.Bishop, Richard P.[Development of sero-diagnostic and molecular tools for the control of important tick-borne pathogens of cattle in Africa]Development of sero-diagnostic and molecular tools for the control of important tick-borne pathogens of cattle in Africa

A 69-kDa immunodominant protein of Trypanosoma congolense was identified as a member of the hsp70 family that is homologous to mammalian BiP. We report here the expression of the gene encoding the T. congolense BiP in a bacterial system. Dot blotting of the truncated recombinant proteins confirme...


Boulange, A.F.Katende, J.Authié, E.[Trypanosoma congolense: Expression of a heat shock protein 70 and initial evaluation as a diagnostic antigen for bovine trypanosomosis]Trypanosoma congolense: Expression of a heat shock protein 70 and initial evaluation as a diagnostic antigen for bovine trypanosomosis

Current serological tests for Babesia bigemina use semipurified merozoite antigens derived from infected erythrocytes. One of the major drawbacks of these tests is that antigen quality can vary from batch to batch. Since the quality of the antigen contributes to the sensitivity and specificity of...


Tebele, N.Skilton, Robert A.Katende, J.Wells, C.W.Nene, VishvanathMcElwain, T.F.Morzaria, S.P.Musoke, A.J.[Cloning, characterization and expression of a 200 Kilodalton diagnostic antigen of Babesia bigemina]Cloning, characterization and expression of a 200 Kilodalton diagnostic antigen of Babesia bigemina
Mamman, M.Katende, J.Moloo, S.K.Peregrine, A.S.[Variation in sensitivity of Trypanosoma congolense to diminazene during the early phase of tsetse-transmitted infection in goats]Variation in sensitivity of Trypanosoma congolense to diminazene during the early phase of tsetse-transmitted infection in goats
Buscher, G.Katende, J.Otim, B.Morrison, W. Ivan[Infection of the host cells of Theileria annulata with T. parva]Infection of the host cells of Theileria annulata with T. parva

Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p15...


Katende, J.Morzaria, S.P.Toye, Philip G.Skilton, Robert A.Nene, VishvanathNkonge, C.Musoke, A.J.[An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule]An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule

Two recombinant vaccines against Theileria parva, based on a near full-length version of the sporozoite surface antigen p67 (p67635), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one i...


Musoke, A.J.Rowlands, G.J.Nene, VishvanathNyanjui, J.Katende, J.Spooner, P.R.Mwaura, S.Odongo, D.Nkonge, C.Mbogo, S.Bishop, Richard P.Morzaria, S.P.[Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge]Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge

Mice were immunized with either an isometamidium-human serum albumin (HSA) conjugate or an isometamidium-porcine thyroglobulin conjugate (PTG). Thereafter, monoclonal antibodies (MAbs) IL-A 1001, IL-A 1002, IL-A 1003, 5F7.B7, and 5F7.C9 were generated and selected on the basis that they recognize...


Peregrine, A.S.Eisler, M.C.Katende, J.Flynn, J.N.Gault, E.A.Kinabo, L.D.B.Holmes, P.H.[Generation of monoclonal antibodies to the anti-trypanosomal drug isometamidium]Generation of monoclonal antibodies to the anti-trypanosomal drug isometamidium

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