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We report the first identification of novel viruses, and sequence of an entire viral genome, by a single step of high-throughput parallel sequencing of small RNAs from diseased, as well as symptomless plants. Contigs were assembled from sequenced total siRNA from plants using small sequence assem...


Kreuze, J.F.Pérez, A.Untiveros, M.Quispe, D.Fuentes, S.Barker, I.Simon, R.[Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses.]Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses.

The plant virus family Flexiviridae includes the definitive genera Potexvirus, Mandarivirus, Allexivirus, Carlavirus, Foveavirus, Capillovirus, Vitivirus, Trichovirus, the putative genus Citrivirus, and some unassigned species. Its establishment was based on similarities in virion morphology, com...


Martelli, G.P.Adams, M.J.Kreuze, J.F.Dolja, V.V.[Family Flexiviridae: A case study in virion and genome plasticity.]Family Flexiviridae: A case study in virion and genome plasticity.

The complete nucleotide sequence of the isolate C1 of Sweet potato feathery mottle virus (SPFMV) strain C and the 5' region of several other strains were determined and analyzed together with the sequences of isolates representing the EA, RC and O strains. This provided molecular evidence for the...


Untiveros, M.Quispe, D.Kreuze, J.F.[Analysis of complete genomic sequences of isolates of the sweet potato feathery mottle virus strains C and EA: molecular evidence for two distinct potyvirus species and two P1 protein domains.]Analysis of complete genomic sequences of isolates of the sweet potato feathery mottle virus strains C and EA: molecular evidence for two distinct potyvirus species and two P1 protein domains.

The complete bipartite genome (RNA1 and RNA2) of a new nepovirus infecting potato was obtained using small RNA sequencing and assembly complemented by Sanger sequencing. Each RNA encodes a single polyprotein, flanked by 5’ and 3’ untranslate regions (UTR) and followed by a poly (A) tail. The puta...


Souza, Joao deMuller, G.Pérez, W.Cuéllar, Wilmer J.Kreuze, J.F.[Complete sequence and variability of a new subgroup B nepovirus infecting potato in central Peru]Complete sequence and variability of a new subgroup B nepovirus infecting potato in central Peru
Souza, J. deFuentes, S.Savenkov, E.I.Cuéllar, Wilmer J.Kreuze, J.F.[The complete nucleotide sequence of sweet potato C6 virus : a carlavirus lacking a cysteine-rich protein]The complete nucleotide sequence of sweet potato C6 virus : a carlavirus lacking a cysteine-rich protein

Wu, S.Lau, K.H.Cao, Q.Hamilton, J.P.Sun, H.Zhou, C.Eserman, L.Gemenet, D.Olukolu, B.A.Wang, H.Crisovan, E.Godden, G.Jiao, C.Wang, X.Kitavi, M.Manrique-Carpintero, N.Vaillancourt, B.Wiegert-Rininger, K.Yang, X.Bao, K.Schaff, J.Kreuze, J.F.Gruneberg, W.J.Khan, A.Ghislain, M.Ma, D.Jiang, J.Mwanga, R.O.M.Leebens-Mack, J.Coin, L.Yencho, G.C.Buell, R.Fei, Z.[Genome sequences of two diploid wild relatives of cultivated sweetpotato reveal targets for genetic improvement.]Genome sequences of two diploid wild relatives of cultivated sweetpotato reveal targets for genetic improvement.
Souza Richards, R.Adams, I.P.Kreuze, J.F.Souza, J. deCuéllar, Wilmer J.Dullemans, A.M.Vlugt, René A.A. van deGlover, R.Hany, U.Dickinson, M.Boonham, N.[The complete genome sequences of two isolates of potato black ringspot virus and their relationship to other isolates and nepoviruses.]The complete genome sequences of two isolates of potato black ringspot virus and their relationship to other isolates and nepoviruses.

The discovery of the insertion of IbT-DNA1 and IbT-DNA2 into the cultivated (hexaploid) sweetpotato [Ipomoea batatas (L.) Lam.] genome constitutes a clear example of an ancient event of Horizontal Gene Transfer (HGT). However, it remains unknown whether the acquisition of both IbT-DNAs by the cul...


Quispe-Huamanquispe, D.G.Gheysen, G.Yang, Y.Jarret, R.Rossel, G.Kreuze, J.F.[The horizontal gene transfer of Agrobacterium T-DNAs into the series Batatas (Genus Ipomoea) genome is not confined to hexaploid sweetpotato.]The horizontal gene transfer of Agrobacterium T-DNAs into the series Batatas (Genus Ipomoea) genome is not confined to hexaploid sweetpotato.

Kreuze, J.F.Koening, RSouza, J. deVetten, H.J.Muller, GFlores, B.Ziebell, HCuéllar, Wilmer J.[The complete genome sequences of a Peruvian and a Colombian isolate of Andean potato latent virus and partial sequences of further isolates suggest the existence of two distinct potato-infecting tymovirus species]The complete genome sequences of a Peruvian and a Colombian isolate of Andean potato latent virus and partial sequences of further isolates suggest the existence of two distinct potato-infecting tymovirus species

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