Recombinant bovine IFN gamma is a potent inhibitor of Cowdria ruminantium growth in vitro irrespective of the rickettsial stock, or the origin of the endothelial cells. These results suggest an important role for IFN gamma in protective immune responses against C. ruminantium infections. Here we ...
Totte, P.
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Vachiery, N.
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Martínez, D.
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Trap, I.
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Ballingall, K.T.
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MacHugh, Niall D.
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Bensaid, A.
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Werenne, J.
,
[Recombinant bovine interferon gamma inhibits the growth of Cowdria ruminantium but fails to induce major histocompatibility complex class II following infection of endothelial cells]
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Recombinant bovine interferon gamma inhibits the growth of Cowdria ruminantium but fails to induce major histocompatibility complex class II following infection of endothelial cells
Dendritic cells (DC) constitute the most effective immune cell population for priming and recalling T cell responses to foreign antigens. DC patrol the peripheral tissues collecting foreign antigen for subsequent presentation by classical class II MHC molecules to T cells in the draining lymph no...
Ballingall, K.T.
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MacHugh, Niall D.
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Taracha, E.L.N.
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Mertens, B.
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McKeever, B.D.
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[Transcription of the unique ruminant class II major histocompatibility complex-DYA and DIB genes in dendritic cells]
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Transcription of the unique ruminant class II major histocompatibility complex-DYA and DIB genes in dendritic cells
We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effecti...
Ballingall, K.T.
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Mwangi, D.M.
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MacHugh, Niall D.
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Taracha, E.L.N.
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Totte, P.
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McKeever, Declan J.
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[A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells]
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A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells
We describe the characterization of two subsets of bovine gamma delta T cells having distinct cell surface phenotype and tissue distribution. One population expresses the previously described 215000 MW WC1 antigen and is negative for the cell-surface differentiation antigens CD2. CD4, and CD8. Th...
MacHugh, Niall D.
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Mburu, J.K.
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Carol, M.J.
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Wyatt, C.R.
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Orden, J.A.
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Davis, W.C.
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[Identification of two distinct subsets of gamma/delta T cells with unique cell surface phenotype and tissue distribution]
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Identification of two distinct subsets of gamma/delta T cells with unique cell surface phenotype and tissue distribution
The studies reported here describe methodology permiting the direct identification of antigens recognized by cytotoxic T lymphocytes. We demonstrated that bovine alloreactive CTL can detect a bovine MHC molecule transiently expressed in a COS cell population in a standard microcytotoxicity assay....
Toye, Philip G.
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Wijngaard, P.L.J.
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MacHugh, Niall D.
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Clevers, H.
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[An assay for the identification of antigens recognized by cytotoxic T cells, based on the transient transfection of COS cells]
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An assay for the identification of antigens recognized by cytotoxic T cells, based on the transient transfection of COS cells
Emery, D.L.
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MacHugh, Niall D.
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Ellis, J.A.
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[The properties and functional activity of non-lymphoid cells from bovine afferent (peripheral) lymph]
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The properties and functional activity of non-lymphoid cells from bovine afferent (peripheral) lymph