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The biological effects of affinity purified seed lectin from African yam bean, Sphenostylis stenocarpa (Harms), were tested on Clavigralla tomentosicollis (Stål) in an artificial seed system. S. stenocarpa was extracted from two African yam bean accessions: Enugu 95-3 and Enugu 98-2. Lectins from...


Okeola, O.G.Machuka, J.[Biological effects of African yam bean lectins on the cowpea coreid bug, Clavigralla tomentosicollis (Stal)]Biological effects of African yam bean lectins on the cowpea coreid bug, Clavigralla tomentosicollis (Stal)

The cowpea beetle, Callosobruchus maculatus, and pod-sucking bug, Clavigralla tomentosicollis, are two of the major insect pests of cowpea in Africa. A lectin was purified from the seeds of the African yam bean (AYB), Sphenostylis stenocarpa, by affinity chromatography on Galactosc-Sepharose 4B. ...


Okeola, O.G.Machuka, J.Fasidi, I.O.[Insecticidal activities of the African yam bean seed lectin on the development of the cowpea beetle and the pod sucking bug]Insecticidal activities of the African yam bean seed lectin on the development of the cowpea beetle and the pod sucking bug
Autor: Machuka, J.

Crops' incompatibility makes conventional breeding approaches untenable in transferring available insect resistance From wild Vigna sp. into cowpea. The alternative recourse is to isolate and transfer alien resistance genes using genetic transformation. This has the added advantage of using usefu...


Machuka, J.[Potential role of transgenic approaches in the control of cowpea insect pests]Potential role of transgenic approaches in the control of cowpea insect pests

Over the last three decades, sporadic efforts have been made to develop regeneration and transformation systems in cowpea ( Vigna unguiculata L. Walp). This paper reviews the progress made to date, including highlights of culture media and explants used for regeneration and chimeric gene construc...


Machuka, J.Adesoye, A.Obembe, O.O.[Regeneration and genetic transformation in cowpea]Regeneration and genetic transformation in cowpea

Seed proteins were extracted from the African yam bean (AYB; Sphenostylis stenocarpa), an underutilized West African food legume. One- and two-dimensional polyacrylamide gel electrophoresis was then used to analyze the albumin fraction, galactose-specific lectins purified on immobilized galactose...


Machuka, J.Okeola, O.[One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins]One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins
Autor: Machuka, J.

This paper reports the preliminary characterization of seed protein fractions from seven varieties of velvet beans (Mucuna pruriens) grown in Nigeria, using sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE). Three of the most abundant polypeptides, with approximate Mr of 23, 2...


Machuka, J.[Characterization of the seed proteins of velvet bean (Mucuna pruriens) from Nigeria]Characterization of the seed proteins of velvet bean (Mucuna pruriens) from Nigeria

Artificial feeding assays were used to study the effect of purified galactose-specific lectins from African yam beans (Sphenostylis stenocarpa) on development of larvae of the cowpea weevil, Callosobruchus maculatus (Coleoptera: Bruchidae) and the legume pod-borer, Maruca vitrata (Lepidoptera: Py...


Machuka, J.Okeola, O.Chrispeels, M.[The African yam bean seed lectin affects the development of the cowpea weevil but does not affect the development of larvae of the legume pod borer]The African yam bean seed lectin affects the development of the cowpea weevil but does not affect the development of larvae of the legume pod borer
Masiga, C.W.Mugoya, C.Ali, R.Mohamed, A.Osama, S.Ngugi, A.Kiambi, D.Villiers, S. deNgugi, K.Niyibigira, T.Tesfamichel, A.Machuka, J.Oduor, R.Runo, S.Adam, R.Matheka, J.Bedada, L.Seth, M.Kuria, E.Ndirigwe, J.Ndolo, P.Muthamia, Z.Nasona, B.Ntimpirangeza, M.Tsegaye, E.Desterio, N.Ogero, K.Mburugu, G.Mukasa, S.Kim, D.J.Ferguson, M.Mneney, E.Nsubuga, E.Rishurimuhirwa, T.Byamugisha, D.Wamatsembe, I.Nzuki, I.Mkamilo, G.S.Kimata, B.Ketema, S.[Enhanced utilization of biotechnology research and development innovations in Eastern and Central Africa for agroecological intensification]Enhanced utilization of biotechnology research and development innovations in Eastern and Central Africa for agroecological intensification

The legume pod‐borer Maruca vitrata (Fabricius), [Lepidoptera: Pyralidae] is a major constraint restricting increased cowpea production in tropical Africa and Asia. Since lectins are known to have insecticidal properties against several pests, a survey was undertaken to screen for the effects of ...


Machuka, J.Damme, E.V.Peumans, W.J.Jackai, L.E.N.[Effect of plant lectins on larval development of legume pod borer, Maruca vitrata]Effect of plant lectins on larval development of legume pod borer, Maruca vitrata

Sweetpotato (Ipomoea batatas) is one of the most important food crops in tropical and subtropical countries. In Sub-Saharan Africa, sweetpotato is mainly produced for consumption and as a source of income by resource-poor farmers. However, their production is limited by severe damage caused by pe...


Manrique Trujillo, S.Wamalwa, N.L.Díaz, D.Reano, R.Tovar, J.C.Prentice, K.Ormachea, M.Kreuze, J.F.Machuka, J.Ghislain, M.[Enhancing resistance against African weevils through development of transgenic sweetpotato cultivars (Ipomoea batatas (L.) Lam.) expressing cry7Aa1, cry3Ca1 and ET33-34 genes.]Enhancing resistance against African weevils through development of transgenic sweetpotato cultivars (Ipomoea batatas (L.) Lam.) expressing cry7Aa1, cry3Ca1 and ET33-34 genes.

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