The biological effects of affinity purified seed lectin from African yam bean, Sphenostylis stenocarpa (Harms), were tested on Clavigralla tomentosicollis (Stål) in an artificial seed system. S. stenocarpa was extracted from two African yam bean accessions: Enugu 95-3 and Enugu 98-2. Lectins from...
Seed proteins were extracted from the African yam bean (AYB; Sphenostylis stenocarpa), an underutilized West African food legume. One- and two-dimensional polyacrylamide gel electrophoresis was then used to analyze the albumin fraction, galactose-specific lectins purified on immobilized galactose...
Machuka, J.
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Okeola, O.
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[One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins]
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One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins
This paper reports the preliminary characterization of seed protein fractions from seven varieties of velvet beans (Mucuna pruriens) grown in Nigeria, using sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE). Three of the most abundant polypeptides, with approximate Mr of 23, 2...
Machuka, J.
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[Characterization of the seed proteins of velvet bean (Mucuna pruriens) from Nigeria]
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Characterization of the seed proteins of velvet bean (Mucuna pruriens) from Nigeria
Artificial feeding assays were used to study the effect of purified galactose-specific lectins from African yam beans (Sphenostylis stenocarpa) on development of larvae of the cowpea weevil, Callosobruchus maculatus (Coleoptera: Bruchidae) and the legume pod-borer, Maruca vitrata (Lepidoptera: Py...
Machuka, J.
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Okeola, O.
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Chrispeels, M.
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[The African yam bean seed lectin affects the development of the cowpea weevil but does not affect the development of larvae of the legume pod borer]
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The African yam bean seed lectin affects the development of the cowpea weevil but does not affect the development of larvae of the legume pod borer
The legume pod‐borer Maruca vitrata (Fabricius), [Lepidoptera: Pyralidae] is a major constraint restricting increased cowpea production in tropical Africa and Asia. Since lectins are known to have insecticidal properties against several pests, a survey was undertaken to screen for the effects of ...
Machuka, J.
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Damme, E.V.
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Peumans, W.J.
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Jackai, L.E.N.
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[Effect of plant lectins on larval development of legume pod borer, Maruca vitrata]
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Effect of plant lectins on larval development of legume pod borer, Maruca vitrata
Wamalwa, L.N.
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Cheseto, X.
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Ouna, E.
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Kaplan, F.
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Maniania, N.K.
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Machuka, J.
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Torto, B.
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Ghislain, M.
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[Toxic Ipomeamarone accumulation in healthy parts of sweetpotato (Ipomoea batatas L. Lam) storage roots upon infection by Rhizopus stolonifer]
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Toxic Ipomeamarone accumulation in healthy parts of sweetpotato (Ipomoea batatas L. Lam) storage roots upon infection by Rhizopus stolonifer