Acute Trypanosoma congolense infection induced moderate, transient anemia in N'Dama cattle (trypanotolerant) and severe anemia in Boran cattle (trypanosusceptible). Erythropoietin receptor (EpoR) was cloned and sequenced from the two breeds of cattle. A single position mutation of Tyr in the Bora...
Suliman, H.B.
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Logan-Henfrey, L.L.
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Majiwa, P.A.O.
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Ole-MoiYoi, O.K.
,
Feldman, B.F.
,
[Analysis of erythropoietin and erythropoietin receptor genes expression in cattle during acute infection with Trypanosoma congolense]
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Analysis of erythropoietin and erythropoietin receptor genes expression in cattle during acute infection with Trypanosoma congolense
A monoclonal antibody (MAb)Tv27 employed in an antigen-detection enzyme immunosorbent assay (Ag-ELISA) for diagnosis of Trypanosoma vivax infection was shown to react with a T. vivax-specific protein of an approximate molecular weight of 10 kDa. This protein is diffusely distributed throughout th...
Masake, R.A.
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Ole-MoiYoi, O.K.
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Urakawa, T.
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Hirumi, H.
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Majiwa, P.A.O.
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Wells, C.W.
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Minja, S.H.
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Makau, J.M.
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Nantulya, V.M.
,
[Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals]
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Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals
The complete nucleotide sequences were determined for three transcripts each encoding a different variant surface glycoprotein (VSG) of Trypanosoma (Nannomonas) congolense. The nucleotide sequence was determined also for a transcript encoding a fourth VSG, but this was truncated. The data obtaine...
A complementary DNA encoding the variant surface glycoprotein (VSG) of Trypanosoma evansi Rode Trypanozoon antigenic type (RoTat)1.2, currently used for experimental serological diagnosis of T. evansi infection in livestock, was cloned as a recombinant plasmid and sequenced. A recombinant baculov...
Urakawa, T.
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Verloo, D.
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Moens, L.
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Buscher, P.
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Majiwa, P.A.O.
,
[Trypanosoma evansi: Cloning and expression in Spodoptera fugiperda insect cells of the diagnostic antigen RoTat1.2]
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Trypanosoma evansi: Cloning and expression in Spodoptera fugiperda insect cells of the diagnostic antigen RoTat1.2
Using a novel multilocus DNA marker analysis method, we studied the population genetic structure of Trypansoma brucei stocks and derived clones isolated from animal and rhodesiense sleeping sickness patients during a national sleeping sickness control program in Mukono district, Uganda. We then ...
Majiwa, P.A.O.
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Hamers, R.
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Meirvenne, N. van
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Matthyssens, G.
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[Evidence for genetic diversity in Trypanosoma (Nannomonas) congolense]
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Evidence for genetic diversity in Trypanosoma (Nannomonas) congolense
Knowles, G.
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Betschart, K.
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Kukla, B.A.
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Scott, J.R.
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Majiwa, P.A.O.
,
[Genetically discrete populations of Trypanosoma congolense from livestock on the Kenyan coast]
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Genetically discrete populations of Trypanosoma congolense from livestock on the Kenyan coast
The variable antigen type (VAT) RoTat 1.2 has been cloned from a T. evansi strain, isolated in 1982 from a water buffalo in Indonesia. All T. evansi isolates hitherto tested express this VAT. In a study on the differential diagnosis of T. equiperdum and T. evansi in horses, we investigated serolo...
Claes, F.
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Verloo, D.
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Waal, D.T. de
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Urakawa, T.
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Majiwa, P.A.O.
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Goddeeris, B.M.
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Buscher, P.
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[Expression of RoTat 1.2 cross-reactive variable antigen type in Trypanosoma evansi and T. equiperdum]
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Expression of RoTat 1.2 cross-reactive variable antigen type in Trypanosoma evansi and T. equiperdum
Drug resistance in trypanosomes is increasingly being recognised as a constraint to livestock production in many parts of Africa. However the long-term stability of such resistance is unclear and has therefore been investigated over a 4-year period at a site in Ethiopia. The result shows that the...
Peregrine, A.S.
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Majiwa, P.A.O.
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Rowlands, G.J.
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[Long-term occurrence of multiple drug resistance in Trypanosoma congolense at Ghibe, Ethiopia]
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Long-term occurrence of multiple drug resistance in Trypanosoma congolense at Ghibe, Ethiopia