Four Boran cattle were infected with Trypanosoma brucei using Glossina morsitans centralis and were left untreated throughout the experimental period of 18 months. During this period, sequential blood samples were collected and examined for the presence of anti-trypanosome antibodies and their an...
Masake, R.A.
,
Moloo, S.K.
,
Nantulya, V.M.
,
Minja, S.H.
,
Makau, J.M.
,
Njuguna, J.T.
,
[Comparative sensitivity of antigen-detection enzyme immunosorbent assay and the microhaematocrit centrifugation technique in the diagnosis of Trypanosoma brucei infections in cattle]
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Comparative sensitivity of antigen-detection enzyme immunosorbent assay and the microhaematocrit centrifugation technique in the diagnosis of Trypanosoma brucei infections in cattle
A monoclonal antibody (MAb)Tv27 employed in an antigen-detection enzyme immunosorbent assay (Ag-ELISA) for diagnosis of Trypanosoma vivax infection was shown to react with a T. vivax-specific protein of an approximate molecular weight of 10 kDa. This protein is diffusely distributed throughout th...
Masake, R.A.
,
Ole-MoiYoi, O.K.
,
Urakawa, T.
,
Hirumi, H.
,
Majiwa, P.A.O.
,
Wells, C.W.
,
Minja, S.H.
,
Makau, J.M.
,
Nantulya, V.M.
,
[Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals]
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Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals
Dot-enzyme linked immunosorbent assay (dot-ELISA), developed for the detection and identification of trypanosome species in tsetse flies, was validated in the laboratory by comparing its sensitivity to that of the polymerase chain reaction (PCR). Tsetse flies were experimentally infected with dif...
Ouma, J.O.
,
Masake, R.A.
,
Masiga, Daniel K.
,
Moloo, S.K.
,
Minja, S.H.
,
Njuguna, J.T.
,
Olaho-Mukani, W.
,
Ndung'u, J.M.
,
[Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)]
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Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)