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Russell, G.C.Marello, K.L.Gallagher, A.McKeever, Declan J.Spooner, R.L.[Amplification and sequencing of expressed DRB second exons from Bos indicus]Amplification and sequencing of expressed DRB second exons from Bos indicus
Flynn, J.N.McKeever, Declan J.Sileghem, M.R.Naessens, Jan[Modulation of the phenotype and function of bovine afferent lymph cells during infection with Trypanosoma congolense]Modulation of the phenotype and function of bovine afferent lymph cells during infection with Trypanosoma congolense

Previous data showed that six out of a group of nine cattle inoculated with NS1-p67, a recombinant form of a 67-KDa Theileria parva sporozoite surface protein, were immune to East Coast fever. This bacterialy expressed antigen encoded all 709 amino acid residues of p67 fused to the C-terminal end...


Nene, VishvanathInumaru, S.McKeever, Declan J.Morzaria, S.P.Shaw, M.Musoke, A.J.[Characterization of an insect cell-derived Theileria parva sporozoite vaccine antigen and immunogenicity in cattle]Characterization of an insect cell-derived Theileria parva sporozoite vaccine antigen and immunogenicity in cattle

Initial laboratory trials of an experimental subunit vaccine against Theileria parva based on the 67-kDa major sporozoite surface antigen revealed a range of responses to challenge. We have analyzed convergence in seven sets of monozygotic twins which suggests that genetic factors may have an i...


Ballingall, K.T.Luyai, A.Rowlands, G.J.Sales, J.Musoke, A.J.Morzaria, S.P.McKeever, Declan J.[Bovine leukocyte antigen major histocompatibility complex class II DRB3*2703 and DRB3*1501 alleles are associated with variation in levels of protection against Theileria parva challenge following immunization with the sporozoite p67 antigen]Bovine leukocyte antigen major histocompatibility complex class II DRB3*2703 and DRB3*1501 alleles are associated with variation in levels of protection against Theileria parva challenge following immunization with the sporozoite p67 antigen

Peripheral blood mononuclear cells (PBMC) from immune cattle proliferate in the presence of autologous Cowdria ruminantium-infected endothelial cells and monocytes. Endothelial cells required treatment with T-cell growth factors to induce class II major histocompatibility complex expression prior...


Mwangi, D.M.Mahan, S.M.Nyanjui, J.K.Taracha, E.L.N.McKeever, Declan J.[Immunization of cattle by infection with Cowdria ruminantium elicits T lymphocytes that recognize autologous, infected endothelial cells and monocytes]Immunization of cattle by infection with Cowdria ruminantium elicits T lymphocytes that recognize autologous, infected endothelial cells and monocytes

The p67 sporozoite antigen of Theileria parva has been fused to the C-terminal secretion signal of Escherichia coli hemolysin and expressed in secreted form by attenuated Salmonella dublin areA strain SL5631. The recombinant p67 antigen was detected in the supernatant of transformed bacterial cul...


Gentschev, I.Glaser, I.Goebel, W.McKeever, Declan J.Musoke, A.J.Heussler, V.T.[Delivery of the p67 sporozoite antigen of Theileria parva by using recombinant Salmonella dublin: Secretion of the product enhances specific antibody responses in cattle]Delivery of the p67 sporozoite antigen of Theileria parva by using recombinant Salmonella dublin: Secretion of the product enhances specific antibody responses in cattle

Cowdria-specific CD4<sup(+)> T-cell lines generated from immunized cattle responded to both soluble and membrane proteins of the agent. The lines produced the Cowdria-inhibitory cytokine IFN-<gamma> in response to soluble antigens fractionated by gel filtration and fast performance liquid chromat...


Totte, P.Nyanjui, J.Bensaid, A.McKeever, Declan J.[Bovine CD4<sup(+)> T-cell lines reactive with soluble and membrane antigens of Cowdria ruminantium]Bovine CD4<sup(+)> T-cell lines reactive with soluble and membrane antigens of Cowdria ruminantium

Theileria parva causes an acute lympho-proliferative disease in cattle, which can result in death of susceptible animals within 2-3 weeks of infection. Analyses of the cellular response in the lymph node draining the site of infection demonstrated an early T cell response, with the appearance of ...


Houston, E.F.Taracha, E.L.N.Brackenbury, L.MacHugh, Niall D.McKeever, Declan J.Charleston, B.Morrison, W. Ivan[Infection of cattle with Theileria parva induces an early CD8 T cell response lacking appropriate effector function]Infection of cattle with Theileria parva induces an early CD8 T cell response lacking appropriate effector function
McKeever, Declan J.[The role of class I MHC-restricted cytotoxic T cells in protection on immune cattle from Theileria parva]The role of class I MHC-restricted cytotoxic T cells in protection on immune cattle from Theileria parva

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