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Interleukin-3(IL-3) is one of the cytokines that act during the early and late stages of blood cell formation. To enable the study of the role of IL-3 in bovine haemopoietic stem cell differentiation, the polymerase chain reaction was used to amplify an IL-3 cDNA from first-strand cDNAs prepared ...


Mwangi, S.M.Logan-Henfrey, L.L.McInnes, C.Mertens, B.[Cloning of the bovine interleukin-3-encoding cDNA. (Colony-stimulating factor: Cytokine: PCR: Neuclotide sequence: RACE)]Cloning of the bovine interleukin-3-encoding cDNA. (Colony-stimulating factor: Cytokine: PCR: Neuclotide sequence: RACE)

A lambda gtll cDNA library was constructed using mRNA isolated from Theileria parva-infected bovine lymphocytes. Sequencing of random clones of this library resulted in the identification of a cDNA encoding bovine interleukin-16 (IL-16). The cDNA has an open reading frame of 1134 bp, and a 3? unt...


Mertens, B.Bishop, Richard P.Taracha, E.A.Morzaria, S.P.Nene, VishvanathSavadye, D.[Cloning, sequence and mRNA expression of bovine interleukin-16]Cloning, sequence and mRNA expression of bovine interleukin-16

The nucleotide (nt) sequence encoding the bovine homologue of interleukin-5 (boIL-5) was determined. Total cDNA generated from stimulated bovine peripheral blood lymphocytes was used to amplify the boIL-5 gene using primers based on the 5' and 3' untranslated regions of the ovine IL-5 cDNA sequen...


Mertens, B.Gobright, E.I.Seow, H.F.[The nucleotide sequence of the bovine interleukin-5-encoding cDNA]The nucleotide sequence of the bovine interleukin-5-encoding cDNA

African trypanosomes are important pathogens of both humans and livestock. We investigated the association of cytokine responses with disease susceptibility in Trypanosoma congolense-infected cattle. Changes in interleukin (IL)-1 beta, IL-2, IL-4, IL-5, IL-6, IL-12 p40, tumor necrosis factor-alph...


Mertens, B.Taylor, K.Muriuki, C.Rocchi, M.[Cytokine mRNA profiles in trypanotolerant and trypanosusceptible cattle infected with the protozoan parasite trypanosoma congolense: Protective role for interleukin-4]Cytokine mRNA profiles in trypanotolerant and trypanosusceptible cattle infected with the protozoan parasite trypanosoma congolense: Protective role for interleukin-4

The cDNA coding for the soluble form of bovine stem cell factor (boSCF-Ala-105) was cloned and recombinant protein was produced in bacteria as a histidine tagged-protein. The protein was purified from the inclusion bodies in one step by metal chelation chromatography under denaturing conditions. ...


Mertens, B.Muriuki, C.Muiya, P.Andrianarivo, A.Mwangi, S.Logan-Henfrey, L.L.[Bovine stem cell factor: Production of a biologically active protein and mRNA analysis in cattle infected with Trypanosoma congolense]Bovine stem cell factor: Production of a biologically active protein and mRNA analysis in cattle infected with Trypanosoma congolense

Infection with African trypanosomes causes the diseases sleeping sickness in humans and nagana in cattle in sub-Saharan Africa. Suppression of cellular immune responses is a feature of trypanosomiasis in bovine, human, and murine hosts. Some aspects of immunosuppression in the murine model are me...


Taylor, K.Lutje, V.Mertens, B.[Nitric Oxide synthesis is depressed in Bos indicus cattle infected with Trypanosoma congolense and Trypanosoma vivax and does not mediate T-cell suppression]Nitric Oxide synthesis is depressed in Bos indicus cattle infected with Trypanosoma congolense and Trypanosoma vivax and does not mediate T-cell suppression

T-cell-mediated immune responses to defined antigens of Trypanosoma congolense were measured in cattle undergoing primary infection. The antigens used were the variable surface glycoprotein and two invariant antigens, a 33-kDa cysteine protease (congopain) and a recombinant form of a 69-kDa heat-...


Lutje, V.Mertens, B.Boulange, A.F.Williams, D.J.L.Authié, E.[Trypanosoma congolense: Proliferative responses and interleukin production in lymph node cells of infected cattle]Trypanosoma congolense: Proliferative responses and interleukin production in lymph node cells of infected cattle

According to studies in human and mouse species it is apparent that both CD40 ligand and tumor necrosis factor alpha (TNF alpha) are members of a novel family of cytokines, referred to as the TNF - superfamily. This article reports the cloning of the cattle CD40 lignad and cattle TNF alpha, and s...


Mertens, B.Muriuki, C.Gaidulis, L.[Cloning of two members of the TNF - superfamily in cattle: CD40 ligand and tumor necrosis factor alpha]Cloning of two members of the TNF - superfamily in cattle: CD40 ligand and tumor necrosis factor alpha

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