For the purpose of constructing yam cDNA libraries, attempts to isolate high quality RNA using several previously reported protocols were unsuccessful. Therefore a protocol was standardized for yam total RNA isolation by using guanidium buffer at the Department of Biology, Virginia Sate Universit...
Anthracnose, caused by Colletotrichum gloeosporioides Penz., is the most severe foliar disease of water yam (Dioscorea alata) worldwide. Population genetic analyses can yield useful insights into the evolutionary potential of C. gloeosporioides and thus lead to the development of appropriate dise...
Abang, Mathew M.
,
Asiedu, Robert
,
Hoffmann, P.
,
Wolf, G.A.
,
Mignouna, H.D.
,
Winter, S.
,
[Pathogenic and genetic variability among Colletotrichum gloeosporioides isolates from different yam hosts in the agroecological zones in Nigeria]
,
Pathogenic and genetic variability among Colletotrichum gloeosporioides isolates from different yam hosts in the agroecological zones in Nigeria
DNA was extracted from the mycelia of Colletotrichum gloeosporioides[Glomerella cingulata] isolates from yam, and amplification reactions using 11 random primers generated 116 DNA bands, 70 of them being informative. The electrophoretic patterns revealed by these primers allowed the identificatio...
Thottappilly, G.
,
Mignouna, H.D.
,
Onasanya, A.
,
Abang, Mathew M.
,
Oyelakin, O.
,
Singh, N.K.
,
[Identification and differentiation of isolates of Colletotrichum gloeosporioides from yam by random amplified polymorphic DNA markers]
,
Identification and differentiation of isolates of Colletotrichum gloeosporioides from yam by random amplified polymorphic DNA markers
Colletotrichum gloeosporioides causes anthracnose, the most severe foliar disease of field-grown water yam (Dioscorea alata). The inheritance of resistance to a moderately virulent (FGS) strain of the pathogen was investigated in crosses between tetraploid D. alata genotypes: TDa 95/00328 (resist...
Mignouna, H.D.
,
Abang, Mathew M.
,
Green, K.R.
,
Asiedu, Robert
,
[Inheritance of resistance in water yam (Disocorea alata) to anthracnose (Colletotrichum gloeosporioides)]
,
Inheritance of resistance in water yam (Disocorea alata) to anthracnose (Colletotrichum gloeosporioides)
Water yam (Dioscorea alata L.) is the most widely cultivated yam species globally. The major limitation to the profitable and sustainable production of D. alata is its susceptibility to anthracnose disease. The availability of resistant varieties could potentially form the cornerstone of an integ...
Abang, Mathew M.
,
Winter, S.
,
Mignouna, H.D.
,
Green, K.R.
,
Asiedu, Robert
,
[Molecular taxonomic, epidemiological and population genetic approaches to understanding yam anthracnose disease]
,
Molecular taxonomic, epidemiological and population genetic approaches to understanding yam anthracnose disease