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Journal Article 1995

Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals

Autor: Masake, R.A. Ole-MoiYoi, O.K. Urakawa, T. Hirumi, H. Majiwa, P.A.O. Wells, C.W. Minja, S.H. Makau, J.M. Nantulya, V.M.

A monoclonal antibody (MAb)Tv27 employed in an antigen-detection enzyme immunosorbent assay (Ag-ELISA) for diagnosis of Trypanosoma vivax infection was shown to react with a T. vivax-specific protein of an approximate molecular weight of 10 kDa. This protein is diffusely distributed throughout th...

Enlace original: https://cgspace.cgiar.org/handle/10568/28608

Investigación en Agricultura

TRYPANOSOMIASIS

TRYPANOSOMA VIVAX

ESCHERICHIA COLI

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Masake, R.A. , Ole-MoiYoi, O.K. , Urakawa, T. , Hirumi, H. , Majiwa, P.A.O. , Wells, C.W. , Minja, S.H. , Makau, J.M. , Nantulya, V.M. , [Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals] , Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals

Conference Paper 1999

Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)

Autor: Ouma, J.O. Masake, R.A. Masiga, Daniel K. Moloo, S.K. Minja, S.H. Njuguna, J.T. Olaho-Mukani, W. Ndung'u, J.M.

Dot-enzyme linked immunosorbent assay (dot-ELISA), developed for the detection and identification of trypanosome species in tsetse flies, was validated in the laboratory by comparing its sensitivity to that of the polymerase chain reaction (PCR). Tsetse flies were experimentally infected with dif...

Enlace original: https://cgspace.cgiar.org/handle/10568/50294

Investigación en Agricultura

TRYPANOSOMIASIS

GLOSSINIDAE

ELISA

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Ouma, J.O. , Masake, R.A. , Masiga, Daniel K. , Moloo, S.K. , Minja, S.H. , Njuguna, J.T. , Olaho-Mukani, W. , Ndung'u, J.M. , [Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)] , Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)

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Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals

Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)