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When compared with other Apicomplexan organisms. Theileria parva has an execptionally small, 10-12 Mbp. genome. There are only 4 chromosomes, each in the Mbp range, and a complete physical map, based on SfiI linking data, is available for each one. A number of genes and cDNAs have been mapped to ...


Nene, VishvanathMorzaria, S.P.Bishop, Richard P.[Organisation and informational content of the Theileria parva genome]Organisation and informational content of the Theileria parva genome

Theileria are tick-transmitted, haemoprotozoan parasites infecting wild and domestic ungulates throughout many areas of the world. The most economically important species are T. parva and T. annulata which are pathogenic to cattle, and T. lestoquardi (syn hirci) which is pathogenic to sheep and g...


Skilton, Robert A.Musoke, A.J.Nene, VishvanathWasawo, D.P.S.Wells, C.W.Spooner, P.R.Bishop, Richard P.Osaso, J.Nkonge, C.Latif, A.Morzaria, S.P.[Molecular characterisation of a Theileria lestoquardi gene encoding a candidate sporozoite vaccine antigen]Molecular characterisation of a Theileria lestoquardi gene encoding a candidate sporozoite vaccine antigen

Theileria annulata and Theileria parva both poses a major surface antigen on the sporozoite stage of the life-cycle, called SPAG-1 and p67, respectively. In each case, these antigens are vaccine candidates and have been shown to induce a degree of homologous protection in earlier work. These anti...


Hall, R.Boulter, N.R.Brown, C.G.D.Wilkie, G.Kirvar, E.Nene, VishvanathMusoke, A.J.Glass, E.J.Morzaria, S.P.[Reciprocal cross-protection induced by sporozoite antigens SPAG-1 from Theileria annulata and p67 from Theileria parva]Reciprocal cross-protection induced by sporozoite antigens SPAG-1 from Theileria annulata and p67 from Theileria parva

Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p15...


Katende, J.Morzaria, S.P.Toye, Philip G.Skilton, Robert A.Nene, VishvanathNkonge, C.Musoke, A.J.[An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule]An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule

The 'Muguga cocktail' which is composed of three Theileria parva stocks Muguga. Kiambu 5 and Serengeti-transformed has been used extensively for live vaccination against East Coast fever in cattle in eastern, central and southern Africa. Herein we describe the molecular characterization of the T....


Bishop, Richard P.Geysen, D.Spooner, P.R.Skilton, Robert A.Nene, VishvanathDolan, T.T.Morzaria, S.P.[Molecular and immunological characterisation of Theileria parva stocks which are components of the Muguga cocktail used for vaccination against East Coast fever in cattle]Molecular and immunological characterisation of Theileria parva stocks which are components of the Muguga cocktail used for vaccination against East Coast fever in cattle

Theileria parva is the causal agent of East Coast fever (ECF), a fatal disease of cattle characterized by pyrexia, transient lymphadenopathy and panleukopenia. We have evaluated monoclonal antibodies (mAb) against three distinct antigens (p67, PIM and p32) of the parasite as immunohistological re...


Honda, Y.Matsubara, Y.Morzaria, S.P.McKeever, Declan J.[Immunohistochemical detection of parasite antigens in Theileria parva-infected bovine lymphocytes]Immunohistochemical detection of parasite antigens in Theileria parva-infected bovine lymphocytes

Theileria parva is an intracellular sporozoan parasite that infects and transforms bovine lymphocytes, causing a severe lymphoproliferative disease known as East Coast fever in eastern, central and southern Africa. In this article, Declan McKeever and colleagues summarize the current understandin...


McKeever, Declan J.Taracha, E.L.N.Morrison, W. IvanMusoke, A.J.Morzaria, S.P.[Protective immune mechanisms against Theileria parva: Evolution of vaccine development strategies]Protective immune mechanisms against Theileria parva: Evolution of vaccine development strategies

Concerted evolution of multicopy gene families in vertebrates is recognized as an important force in the generation of biological novelty but has not been documented for the multicopy genes of protozoa. A multicopy locus, Tpr, which consists of tandemly arrayed open reading frames (ORFs) containi...


Bishop, Richard P.Musoke, A.J.Morzaria, S.P.Sohanpal, B.K.Gobright, E.I.[Concerted evolution at a multicopy locus in the protozoan parasite Theileria parva: Extreme divergence of potential protein-coding sequences]Concerted evolution at a multicopy locus in the protozoan parasite Theileria parva: Extreme divergence of potential protein-coding sequences

Full-length recombinant versions of p67, the 709 amino acid major surface protein of Theileria parva sporozoites, induce immunity to East Coast fever (ECF) in cattle. We show that a soluble Escherichia coli recombinant version of p67 (p67635), in which a prokaryotic signal peptide replaces the e...


Bishop, Richard P.Nene, VishvanathStaeyert, J.Rowlands, G.J.Nyanjui, J.Osaso, J.Morzaria, S.P.Musoke, A.J.[Immunity to East Coast fever in cattle induced by a polypeptide fragment of the major surface coat protein of Theileria parva sporozoites]Immunity to East Coast fever in cattle induced by a polypeptide fragment of the major surface coat protein of Theileria parva sporozoites
Gardner, M.J.Bishop, Richard P.Shah, TushaarVilliers, Etienne P. deCarlton, J.M.Hall, N.Ren, Q.Paulsen, I.T.Pain, A.Berriman, M.Wilson, R.J.M.Sato, S.Ralph, S.A.Mann, D.J.Xiong, Z.Shallom, S.J.Weidman, J.Jiang, L.Lynn,, J.Weaver, B.Shoaibi, A.Domingo, A.R.Wasawo, D.Crabtree, J.Wortman, J.R.Haas, B.Angiuoli, S.V.Creasy, T.H.Lu, C.Suh, B.Silva, J.C.Utterback, T.R.Feldblyum, T.V.Pertea, M.Allen, J.Nierman, W.C.Taracha, E.L.N.Salzberg, S.L.White, O.R.Fitzhugh, H.A.Morzaria, S.P.Venter, J. CraigFraser, C.M.Nene, Vishvanath[Genome sequence of Theileria parva, a bovine pathogen that transforms lymphocytes]Genome sequence of Theileria parva, a bovine pathogen that transforms lymphocytes

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