The use of tick vaccines in mammalian hosts has been shown to be the most promising alternative tick control method to current use of acaricides, which suffers from a number of limitations. However, the success of this method is dependent on the identification cloning, and in vitro expression of ...
Mulenga, A.
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Sugimoto, C.
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Sako, C.
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Ohashi, K.
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Musoke, A.J.
,
Shubash, M.
,
Onuma, M.
,
[Molecular characterization of Haemaphysalis longicornis tick salivary gland-associated 29-kilodalton protein and its effect as a vaccine against tick infestation in rabbits]
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Molecular characterization of Haemaphysalis longicornis tick salivary gland-associated 29-kilodalton protein and its effect as a vaccine against tick infestation in rabbits
A stage-specific surface antigen of Theileria parva, p67, is the basis for the development of an anti-sporozoite vaccine for the control of East Coast fever (ECF) in cattle. By Pepscan analysis with a series of overlapping synthetic p67 peptides, the antigen was shown to contain five distinct lin...
Nene, Vishvanath
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Gobright, E.I.
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Bishop, Richard P.
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Morzaria, S.P.
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Musoke, A.J.
,
[Linear peptide specificity of bovine antibody responses to p67 of Theileria parva and sequence diversity of sporozoite-neutralising epitopes: Implications for a vaccine]
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Linear peptide specificity of bovine antibody responses to p67 of Theileria parva and sequence diversity of sporozoite-neutralising epitopes: Implications for a vaccine
Monoclonal antibodies (MAb) binding to Cowdria ruminantium elementary bodies (CB) were identified by enzyme-linked immunosorbent assay, and surface binding of one MAb (446.15) to intact CB was determined by immunofluorescence, immunogold labeling, and transmission electron microscopy. MAb 446.15 ...
Shompole, S.
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Rurangirwa, F.R.
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Wambugu, J.
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Sitienei, J.
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Mwangi, D.M.
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Musoke, A.J.
,
Mahan, S.
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Wells, C.W.
,
McGuire, T.C.
,
[Monoclonal antibody binding to a surface exposed epitope on Cowdria ruminantium that is conserved among light strains]
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Monoclonal antibody binding to a surface exposed epitope on Cowdria ruminantium that is conserved among light strains
Nantulya, V.M.
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Musoke, A.J.
,
Rurangirwa, F.R.
,
Barbet, A.F.
,
Ngaira, J.M.
,
Katende, J.M.
,
[Immune depression in African trypanosomiasis. The role of antigenic competition]
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Immune depression in African trypanosomiasis. The role of antigenic competition
A flow cytometric method using anti-parasite antibodies was developed to measure binding of theileria parva sporozoites to the target bovine lymphocyte membrane. Parasite-host cell interactions could be inhibited by monoclonal antibodies to bovine MHC class I and partially by one of two antibodie...
Syfrig, J.
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Wells, C.
,
Daubenberger, C.A.
,
Musoke, A.J.
,
Naessens, Jan
,
[Proteolytic cleavage of surface proteins enhances susceptibility of lymphocytes to invasion by Theileria parva sporozoites]
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Proteolytic cleavage of surface proteins enhances susceptibility of lymphocytes to invasion by Theileria parva sporozoites
Vervoort, T.
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Barbet, F.
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Musoke, A.J.
,
Magnus, E.
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Mpimbaza, G.W.N.
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Meirvenne, N. van
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[Isotypic surface glycoproteins of trypanosomes]
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Isotypic surface glycoproteins of trypanosomes
Immunity to Theileria parva infection in cattle is often parasite stock specific. The antigenic diversity which is expressed at the schizont stage of the parasite together with a wild reservoir of the organism in buffalo has complicated the development of effective disease control by immunization...
Nene, Vishvanath
,
Musoke, A.J.
,
Gobright, E.I.
,
Morzaria, S.P.
,
[Conservation of the sporozoite p67 vaccine antigen in cattle-derived Theileria parva stocks with different cross-immunity profiles]
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Conservation of the sporozoite p67 vaccine antigen in cattle-derived Theileria parva stocks with different cross-immunity profiles
Susceptible A/J and more resistant C57BL/6J mice were infected with Plasmodium chabaudi chabaudi 54X, P.c. chabaudi AS and Plasmodium chabaudi adami 408XZ. As expected, most C57BL/6J mice survived the infections with the different isolates. But in contrast to previous observations, not all A/J ...
Hernández Valladares, M.
,
Naessens, Jan
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Nagda, S.M.
,
Musoke, A.J.
,
Rihet, P.
,
Ole-MoiYoi, O.K.
,
Iraqi, F.A.
,
[Comparison of pathology in susceptible A/J and resistant C57BL/6J mice after infection with different sub-strains of Plasmodium chabaudi]
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Comparison of pathology in susceptible A/J and resistant C57BL/6J mice after infection with different sub-strains of Plasmodium chabaudi
To identify the genes encoding novel immunodominant antigens of Theileria parvo a ?gt11 library of piroplasm genomic DNA was immunoscreened with bovine recovery serum and a gene encoding a 150 kDa antigen (p150) was identified. The predicted polypeptide contains an N-terminal secretory signal seq...
Skilton, Robert A.
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Bishop, Richard P.
,
Wells, C.W.
,
Spooner, P.R.
,
Gobright, E.I.
,
Nkonge, C.
,
Musoke, A.J.
,
Macklin, M.D.
,
Iams, K.P.
,
[Cloning and characterization of a 150 kDa microsphere antigen of Theileria parva that is immunologically cross-reactive with the polymorphic immunodominant molecule (PIM)]
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Cloning and characterization of a 150 kDa microsphere antigen of Theileria parva that is immunologically cross-reactive with the polymorphic immunodominant molecule (PIM)