Current serological tests for Babesia bigemina use semipurified merozoite antigens derived from infected erythrocytes. One of the major drawbacks of these tests is that antigen quality can vary from batch to batch. Since the quality of the antigen contributes to the sensitivity and specificity of...
Tebele, N.
,
Skilton, Robert A.
,
Katende, J.
,
Wells, C.W.
,
Nene, Vishvanath
,
McElwain, T.F.
,
Morzaria, S.P.
,
Musoke, A.J.
,
[Cloning, characterization and expression of a 200 Kilodalton diagnostic antigen of Babesia bigemina]
,
Cloning, characterization and expression of a 200 Kilodalton diagnostic antigen of Babesia bigemina
Protein components of the cement cone of ixodid ticks are candidates for inclusion in vaccines against tick infestation, since they are essential for tick attachment and feeding. We describe here the cloning of a cDNA encoding a 36 kDa protein, designated Rhipicephalus Immuno-dominant Molecule 36...
Bishop, Richard P.
,
Lambson, B.
,
Wells, C.
,
Pandit, P.
,
Osaso, J.
,
Nkonge, C.
,
Morzaria, S.P.
,
Musoke, A.J.
,
Nene, Vishvanath
,
[A cement protein of the tick Rhipicephalus appendiculatus, located in the secretory e cell granules of the type III salivary gland acini, induces strong antibody responses in cattle]
,
A cement protein of the tick Rhipicephalus appendiculatus, located in the secretory e cell granules of the type III salivary gland acini, induces strong antibody responses in cattle
Theileria are tick-transmitted, haemoprotozoan parasites infecting wild and domestic ungulates throughout many areas of the world. The most economically important species are T. parva and T. annulata which are pathogenic to cattle, and T. lestoquardi (syn hirci) which is pathogenic to sheep and g...
Skilton, Robert A.
,
Musoke, A.J.
,
Nene, Vishvanath
,
Wasawo, D.P.S.
,
Wells, C.W.
,
Spooner, P.R.
,
Bishop, Richard P.
,
Osaso, J.
,
Nkonge, C.
,
Latif, A.
,
Morzaria, S.P.
,
[Molecular characterisation of a Theileria lestoquardi gene encoding a candidate sporozoite vaccine antigen]
,
Molecular characterisation of a Theileria lestoquardi gene encoding a candidate sporozoite vaccine antigen
Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p15...
Katende, J.
,
Morzaria, S.P.
,
Toye, Philip G.
,
Skilton, Robert A.
,
Nene, Vishvanath
,
Nkonge, C.
,
Musoke, A.J.
,
[An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule]
,
An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule
Two novel baculovirus-derived recombinant Theileria parva p67 constructs were tested for their vaccine potential against East Coast fever. Boran calves were immunized with a his-GFP-p67 fusion protein (GFP:p67DeltaSS) or with GP64:p67C, a protein fusion between a C-terminal domain of p67 and th...
Two recombinant vaccines against Theileria parva, based on a near full-length version of the sporozoite surface antigen p67 (p67635), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one i...
Musoke, A.J.
,
Rowlands, G.J.
,
Nene, Vishvanath
,
Nyanjui, J.
,
Katende, J.
,
Spooner, P.R.
,
Mwaura, S.
,
Odongo, D.
,
Nkonge, C.
,
Mbogo, S.
,
Bishop, Richard P.
,
Morzaria, S.P.
,
[Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge]
,
Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge
Katende, J.M.
,
Musoke, A.J.
,
Nantulya, V.M.
,
Goddeeris, B.M.
,
[A new method for fixation and preservation of trypanosomal antigens for use in the indirect immunofluorescent antibody test for diagnosis of bovine trypanosomiasis]
,
A new method for fixation and preservation of trypanosomal antigens for use in the indirect immunofluorescent antibody test for diagnosis of bovine trypanosomiasis
To define Babesia bigemina-specific antigens on the surface of infected erythrocytes, monoclonal antibodies (MAbs) were identified by live-cell immunofluorescence. As determined by live-cell immunofluorescence, two MAbs made to the Mexico strain reacted with the Mexico strain and three Kenya stra...
Shompole, S.
,
Perryman, L.E.
,
Rurangirwa, F.R.
,
McElwain, T.F.
,
Jasmer, D.P.
,
Musoke, A.J.
,
Wells, C.W.
,
McGuire, T.C.
,
[Monoclonal antibody to a conserved epitope on proteins encoded by Babesia bigemina and present on the surface of intact infected erythrocytes]
,
Monoclonal antibody to a conserved epitope on proteins encoded by Babesia bigemina and present on the surface of intact infected erythrocytes
The p67 sporozoite antigen of Theileria parva has been fused to the C-terminal secretion signal of Escherichia coli hemolysin and expressed in secreted form by attenuated Salmonella dublin areA strain SL5631. The recombinant p67 antigen was detected in the supernatant of transformed bacterial cul...
Gentschev, I.
,
Glaser, I.
,
Goebel, W.
,
McKeever, Declan J.
,
Musoke, A.J.
,
Heussler, V.T.
,
[Delivery of the p67 sporozoite antigen of Theileria parva by using recombinant Salmonella dublin: Secretion of the product enhances specific antibody responses in cattle]
,
Delivery of the p67 sporozoite antigen of Theileria parva by using recombinant Salmonella dublin: Secretion of the product enhances specific antibody responses in cattle