Theileria annulata and Theileria parva both poses a major surface antigen on the sporozoite stage of the life-cycle, called SPAG-1 and p67, respectively. In each case, these antigens are vaccine candidates and have been shown to induce a degree of homologous protection in earlier work. These anti...
Hall, R.
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Boulter, N.R.
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Brown, C.G.D.
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Wilkie, G.
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Kirvar, E.
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Nene, Vishvanath
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Musoke, A.J.
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Glass, E.J.
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Morzaria, S.P.
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[Reciprocal cross-protection induced by sporozoite antigens SPAG-1 from Theileria annulata and p67 from Theileria parva]
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Reciprocal cross-protection induced by sporozoite antigens SPAG-1 from Theileria annulata and p67 from Theileria parva
Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p15...
Katende, J.
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Morzaria, S.P.
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Toye, Philip G.
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Skilton, Robert A.
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Nene, Vishvanath
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Nkonge, C.
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Musoke, A.J.
,
[An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule]
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An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule
Theileria parva is an intracellular sporozoan parasite that infects and transforms bovine lymphocytes, causing a severe lymphoproliferative disease known as East Coast fever in eastern, central and southern Africa. In this article, Declan McKeever and colleagues summarize the current understandin...
McKeever, Declan J.
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Taracha, E.L.N.
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Morrison, W. Ivan
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Musoke, A.J.
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Morzaria, S.P.
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[Protective immune mechanisms against Theileria parva: Evolution of vaccine development strategies]
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Protective immune mechanisms against Theileria parva: Evolution of vaccine development strategies
The major histocompatibility complex (MHC) class I molecules are ubiquious cell surface molecules involved in the cell-mediated immune response. We show here, using a number of different, independent approaches, that these proteins are an essential component of the host cell surface receptor invo...
Shaw, M.K.
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Tilney, L.G.
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Musoke, A.J.
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Teale, A.J.
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[MHC class I molecules are an essential cell surface component involved in Theileria parva sporozoite binding to bovine lymphocytes]
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MHC class I molecules are an essential cell surface component involved in Theileria parva sporozoite binding to bovine lymphocytes
Concerted evolution of multicopy gene families in vertebrates is recognized as an important force in the generation of biological novelty but has not been documented for the multicopy genes of protozoa. A multicopy locus, Tpr, which consists of tandemly arrayed open reading frames (ORFs) containi...
Bishop, Richard P.
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Musoke, A.J.
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Morzaria, S.P.
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Sohanpal, B.K.
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Gobright, E.I.
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[Concerted evolution at a multicopy locus in the protozoan parasite Theileria parva: Extreme divergence of potential protein-coding sequences]
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Concerted evolution at a multicopy locus in the protozoan parasite Theileria parva: Extreme divergence of potential protein-coding sequences
Full-length recombinant versions of p67, the 709 amino acid major surface protein of Theileria parva sporozoites, induce immunity to East Coast fever (ECF) in cattle. We show that a soluble Escherichia coli recombinant version of p67 (p67635), in which a prokaryotic signal peptide replaces the e...
Bishop, Richard P.
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Nene, Vishvanath
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Staeyert, J.
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Rowlands, G.J.
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Nyanjui, J.
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Osaso, J.
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Morzaria, S.P.
,
Musoke, A.J.
,
[Immunity to East Coast fever in cattle induced by a polypeptide fragment of the major surface coat protein of Theileria parva sporozoites]
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Immunity to East Coast fever in cattle induced by a polypeptide fragment of the major surface coat protein of Theileria parva sporozoites
Two novel baculovirus-derived recombinant Theileria parva p67 constructs were tested for their vaccine potential against East Coast fever. Boran calves were immunized with a his-GFP-p67 fusion protein (GFP:p67DeltaSS) or with GP64:p67C, a protein fusion between a C-terminal domain of p67 and th...
Shaw, M.K.
,
Tilney, L.G.
,
Musoke, A.J.
,
[The entry of Theileria parva sporozoites into bovine lymphocytes: Evidence for MHC class I involvement]
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The entry of Theileria parva sporozoites into bovine lymphocytes: Evidence for MHC class I involvement
East Coast fever (ECF) in cattle is caused by the tick-borne protozoan parasite Theileria parva. The major sporozoite surface antigen of T. parva (p67) is an important candidate for inclusion in a subunit vaccine. Recently, we reported the expression and production of different parts of p67 as f...
Two recombinant vaccines against Theileria parva, based on a near full-length version of the sporozoite surface antigen p67 (p67635), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one i...
Musoke, A.J.
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Rowlands, G.J.
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Nene, Vishvanath
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Nyanjui, J.
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Katende, J.
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Spooner, P.R.
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Mwaura, S.
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Odongo, D.
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Nkonge, C.
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Mbogo, S.
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Bishop, Richard P.
,
Morzaria, S.P.
,
[Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge]
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Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge