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For the purpose of constructing yam cDNA libraries, attempts to isolate high quality RNA using several previously reported protocols were unsuccessful. Therefore a protocol was standardized for yam total RNA isolation by using guanidium buffer at the Department of Biology, Virginia Sate Universit...


Narina, S.S.Mohammed, I.Asiedu, RobertMignouna, H.D.[A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction]A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction

Background: Anthracnose (Colletotrichum gloeosporioides) is a major limiting factor in the production of yam (Dioscorea spp.) worldwide. Availability of high quality sequence information is necessary for designing molecular markers associated with resistance. However, very limited sequence inform...


Narina, S.S.Buyyarapu, R.Kottapalli, K.R.Sartie, A.M.Ali, M.I.Asiedu, RobertHodeba, M.Sayre, B.L.Scheffler, B.E.[Generation and analysis of expressed sequence tags (ESTs) for marker development in yam (Dioscorea alata L.)]Generation and analysis of expressed sequence tags (ESTs) for marker development in yam (Dioscorea alata L.)

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