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A visually read dot-enzyme linked immunosorbent asay (dot-ELISA) developed for the detection of trypanosomes in tsetse flies (Glossina spp.) was evaluated in the laboratory and under field conditions. In the evaluation, the fly dissection method was used as a standard technique and compared to th...


Ouma, J.O.Masake, R.A.Masiga, Daniel K.Moloo, S.K.Njuguna, J.T.Ndung'u, J.M.[Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)]Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)

Resistance to the drugs used to control African animal trypanosomosis is increasingly recognised as a constraint to livestock production in sub-Saharan Africa. The most commonly used tests for detection of trypanocidal drug resistance are tests using mice or ruminants, but these suffer from lack ...


Eisler, M.C.Brandt, J.Bauer, B.Clausen, P.H.Delespaux, V.Holmes, P.H.Ilemobade, A.Machila, N.Mbwambo, H.McDermott, John J.Mehlitz, D.Murilla, G.Ndung'u, J.M.Peregrine, A.S.Sidibe, I.Sinyangwe, L.Geerts, S.[Standardised tests in mice and cattle for the detection of drug resistance in tsetse-transmitted trypanosomes of African domestic cattle]Standardised tests in mice and cattle for the detection of drug resistance in tsetse-transmitted trypanosomes of African domestic cattle

Dot-enzyme linked immunosorbent assay (dot-ELISA), developed for the detection and identification of trypanosome species in tsetse flies, was validated in the laboratory by comparing its sensitivity to that of the polymerase chain reaction (PCR). Tsetse flies were experimentally infected with dif...


Ouma, J.O.Masake, R.A.Masiga, Daniel K.Moloo, S.K.Minja, S.H.Njuguna, J.T.Olaho-Mukani, W.Ndung'u, J.M.[Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)]Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)

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