In this film, Vish Nene director of the Vaccines Biosciences program at ILRI, talks about new research that is seeking to create a second-generation vaccine against East Coast fever.
Nene, Vishvanath
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[Battling an African cattle killer: Second-generation vaccine against East Coast fever]
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Battling an African cattle killer: Second-generation vaccine against East Coast fever
Svitek, Nicholas
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Saya, R.
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Awino, Elias
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Gilbert, S.C.
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Poole, Elizabeth J.
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Nene, Vishvanath
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Steinaa, Lucilla
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[Immunogenicity and protection of the Theileria parva CTL antigen Tp1, with or without a leader sequence, using HAd5/MVA prime-boost vaccination]
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Immunogenicity and protection of the Theileria parva CTL antigen Tp1, with or without a leader sequence, using HAd5/MVA prime-boost vaccination
Svitek, Nicholas
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Saya, R.
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Awino, Elias
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Munyao, S.
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Njoroge, T.
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Gilbert, S.
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Nene, Vishvanath
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Steinaa, Lucilla
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[Evaluating the Ad5/MVA antigen delivery systems]
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Evaluating the Ad5/MVA antigen delivery systems
We have recently shown that the BoLA-A18 variant haplotype (BoLA-6*01302) is more prevalent than the BoLA-A18 haplotype (BoLA-6*01301) in a sample of Holstein/Friesian cattle in Kenya. These MHC class I allelic variants differ by a single amino acid polymorphism (Glu97 to Leu97) in the peptide-bi...
Svitek, Nicholas
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Awino, Elias
,
Nene, Vishvanath
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Steinaa, Lucilla
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[BoLA-6*01301 and BoLA-6*01302, two allelic variants of the A18 haplotype, present the same epitope from the Tp1 antigen of Theileria parva]
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BoLA-6*01301 and BoLA-6*01302, two allelic variants of the A18 haplotype, present the same epitope from the Tp1 antigen of Theileria parva
Reassociation kinetics and flow cytometry data indicate that ixodid tick genomes are large, relative to most arthropods, containing ≥ 109 base pairs. The molecular basis for this is unknown. We have identified a novel small interspersed element with features of a tRNA-derived SINE, designated Ruk...
Sunter, J.D.
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Patel, S.P.
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Skilton, Robert A.
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Knowles, Donald P.
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Scoles, Glen A.
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Nene, Vishvanath
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Villiers, Etienne P. de
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Bishop, Richard P.
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[A novel SINE family occurs frequently in both genomic DNA and transcribed sequences in ixodid ticks of the arthropod sub-phylum Chelicerata]
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A novel SINE family occurs frequently in both genomic DNA and transcribed sequences in ixodid ticks of the arthropod sub-phylum Chelicerata
Previous studies using monoclonal antibody (mAb) 4C9 specific for a 32 kDa antigen (p32) of Theileria parva demonstrated expression of the antigen on the surface of the sporozoite, making it a potential antigen for sporozoite neutralization. A full-length cDNA encoding the major merozoite/piropla...
Skilton, Robert A.
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Musoke, A.J.
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Wells, C.W.
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Yagi, Y.
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Nene, Vishvanath
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Spooner, P.R.
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Gachanja, J.
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Osaso, J.
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Bishop, Richard P.
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Morzaria, S.P.
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[A 32 kDa surface antigen of Theileria parva: Characterization and immunization studies]
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A 32 kDa surface antigen of Theileria parva: Characterization and immunization studies
Immunity against the bovine protozoan parasite Theileria parva has previously been shown to be mediated through lysis of parasite-infected cells by MHC class I restricted CD8+ cytotoxic T lymphocytes. It is hypothesized that identification of CTL target schizont antigens will aid the development ...
Graham, S.P.
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Honda, Y.
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Pelle, Roger
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Mwangi, D.M.
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Glew, E.J.
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Villiers, Etienne P. de
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Shah, Tushaar
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Bishop, Richard P.
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Bruggen, P. van der
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Nene, Vishvanath
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Taracha, E.L.N.
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[A novel strategy for the identification of antigens that are recognised by bovine MHC class I restricted cytotoxic T cells in a protozoan infection using reverse vaccinology]
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A novel strategy for the identification of antigens that are recognised by bovine MHC class I restricted cytotoxic T cells in a protozoan infection using reverse vaccinology
Flow cytometry, enzyme-linked immunospot (ELISpot) and cellular cytotoxicity assays are powerful tools for studying the cellular immune response towards intracellular pathogens and vaccines in livestock species. Lymphocytes from immunized animals can be purified using Ficoll-Paque density gradien...
Svitek, Nicholas
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Taracha, E.L.N.
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Saya, R.
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Awino, Elias
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Nene, Vishvanath
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Steinaa, Lucilla
,
[Analysis of the cellular immune responses to vaccines]
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Analysis of the cellular immune responses to vaccines
In this study, we investigated the immunological effects of bovine heat shock protein 70 (HSP70) on the major Theileria sergenti surface protein (p33). The gene encoding p33 was expressed as a fusion protein with bovine HSP70 from a plasmid vector. The adjuvant function of HSP70 on p33 was evalua...
Jeong, W.
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Kweon, C.H.
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Kwang, S.W.
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Hu Suk Lee
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Xu, Y.
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Lu, C.
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Zhang, S.
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Nene, Vishvanath
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[Adjuvant effect of bovine heat shock protein 70 on piroplasm surface protein, p33, of Theileria sergenti]
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Adjuvant effect of bovine heat shock protein 70 on piroplasm surface protein, p33, of Theileria sergenti
Bishop, Richard P.
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Musoke, A.J.
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Skilton, Robert A.
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Morzaria, S.P.
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Gardner, M.
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Nene, Vishvanath
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[Theileria: Life cycle stages associated with the ixodid tick vector]
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Theileria: Life cycle stages associated with the ixodid tick vector