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Kairo, A.Gobright, E.I.Fairlamb, A.H.Nene, Vishvanath[A 7.1 Kb linear DNA molecule of Theileria parva has scrambled rDNA sequences and open reading frames for mitochondrially encoded proteins]A 7.1 Kb linear DNA molecule of Theileria parva has scrambled rDNA sequences and open reading frames for mitochondrially encoded proteins

Protein components of the cement cone of ixodid ticks are candidates for inclusion in vaccines against tick infestation, since they are essential for tick attachment and feeding. We describe here the cloning of a cDNA encoding a 36 kDa protein, designated Rhipicephalus Immuno-dominant Molecule 36...


Bishop, Richard P.Lambson, B.Wells, C.Pandit, P.Osaso, J.Nkonge, C.Morzaria, S.P.Musoke, A.J.Nene, Vishvanath[A cement protein of the tick Rhipicephalus appendiculatus, located in the secretory e cell granules of the type III salivary gland acini, induces strong antibody responses in cattle]A cement protein of the tick Rhipicephalus appendiculatus, located in the secretory e cell granules of the type III salivary gland acini, induces strong antibody responses in cattle

Polymorphic, multicopy gene families are frequently located in subtelomeric regions of the genomes of parasitic protozoa. Theileria parva telomere-associated (TA) DNA from two chromosomes contained long open reading frames (ORFs) 54% identical at the N-termini whose ends were 2670 and 2680 bp fro...


Bishop, Richard P.Gobright, E.I.Nene, VishvanathMorzaria, S.P.Musoke, A.J.Sohanpal, B.K.[Polymorphic open reading frames encoding secretory proteins are located less than 3 kilobases from Theileria parva telomeres]Polymorphic open reading frames encoding secretory proteins are located less than 3 kilobases from Theileria parva telomeres

Ixodid ticks manipulate mammalian host pathways by secreting molecules from salivary glands. Novel cDNAs containing functional secretion signals were isolated from ixodid tick salivary glands using a signal sequence trap. Only 15/61 Rhipicephalus appendiculatus and 1/7 Amblyomma variegatum cDNAs ...


Lambson, B.Nene, VishvanathObura, M.Shah, TushaarPandit, P.Ole-MoiYoi, O.K.Delroux, K.Welburn, S.C.Skilton, Robert A.Villiers, Etienne P. deBishop, Richard P.[Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells]Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells

Because the function of signal sequences has been conserved during evolution it has been possible to develop both bioinformatics resources to identify them and techniques to clone genes that encode secretary proteins. The latter entail insertion of heterologous signals upstream of signal peptide ...


Nene, VishvanathBishop, Richard P.[Trapping parasite secretory proteins in baker's yeast]Trapping parasite secretory proteins in baker's yeast
Iams, K.P.Young, J.R.Nene, VishvanathDesai, J.Webster, P.Ole-MoiYoi, O.K.Musoke, A.J.[Characterization of the gene encoding a 104 kilodalton microneme-rhoptry protein of Theileria parva]Characterization of the gene encoding a 104 kilodalton microneme-rhoptry protein of Theileria parva

Immunity to the bovine apicomplexan parasite Theileria parva is associated with MHC-I restricted CD8+ T cell responses directed against the intralymphocytic schizont stage of the parasite. A number of schizont-stage antigens that are targets of CD8+ T cell responses from immune animals have been ...


Tinega, A.N.Pelle, RogerKang'a, S.Gicheru, M.M.Taracha, E.L.N.Nene, VishvanathGraham, S.P.[Fusion of a cell penetrating peptide from HIV-1 TAT to the Theileria parva antigen Tp2 enhances the stimulation of bovine CD8+ T cell responses]Fusion of a cell penetrating peptide from HIV-1 TAT to the Theileria parva antigen Tp2 enhances the stimulation of bovine CD8+ T cell responses

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