Background Although vector control strategies, such as insecticide-treated bed nets (ITNs) and indoor residual spraying (IRS) have been effective in Kenya the transmission of malaria continues to afflict western Kenya. This residual transmission is driven in part by Anopheles arabiensis, known fo...
Lozano-Fuentes, Saul
,
Kading, R.C.
,
Hartman, D.A.
,
Okoth, Edward A.
,
Githaka, Naftaly W.
,
Nene, Vishvanath
,
Poche, R.M.
,
[Evaluation of a topical formulation of eprinomectin against Anopheles arabiensis when administered to Zebu cattle (Bos indicus) under field conditions]
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Evaluation of a topical formulation of eprinomectin against Anopheles arabiensis when administered to Zebu cattle (Bos indicus) under field conditions
East Coast fever (ECF) caused by Theileria parva kills cattle in East, Central and Southern Africa leading to significant economic losses. Vaccination is used as a control strategy against ECF and is presently dependent on deliberate infection with live sporozoites and simultaneous treatment with...
Nyagwange, James
,
Nene, Vishvanath
,
Mwalimu, Stephen M.
,
Henson, Sonal P.
,
Steinaa, Lucilla
,
Nzau, Benjamin M.
,
Tijhaar, Edwin
,
Pelle, Roger
,
[Antibodies to in silico selected GPI-anchored Theileria parva proteins neutralize sporozoite infection in vitro]
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Antibodies to in silico selected GPI-anchored Theileria parva proteins neutralize sporozoite infection in vitro
Kairo, A.
,
Gobright, E.I.
,
Fairlamb, A.H.
,
Nene, Vishvanath
,
[A 7.1 Kb linear DNA molecule of Theileria parva has scrambled rDNA sequences and open reading frames for mitochondrially encoded proteins]
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A 7.1 Kb linear DNA molecule of Theileria parva has scrambled rDNA sequences and open reading frames for mitochondrially encoded proteins
Tremendous progress has been made over the last ten years on East Coast fever (ECF) research. Publication of a reference genome sequence of Theileria parva, the causative agent of ECF, has led to a more thorough characterization of the genotypic and antigenic diversity of the pathogen. It also fa...
Nene, Vishvanath
,
Kiara, Henry K.
,
Lacasta, Anna
,
Pelle, Roger
,
Svitek, Nicholas
,
Steinaa, Lucilla
,
[The biology of Theileria parva and control of East Coast fever—Current status and future trends]
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The biology of Theileria parva and control of East Coast fever—Current status and future trends
Mycoplasma mycoides subsp. capri (Mmc) and subsp. mycoides (Mmm) are important ruminant pathogens worldwide causing diseases such as pleuropneumonia, mastitis and septicaemia. They express galactofuranose residues on their surface, but their role in pathogenesis has not yet been determined. The M...
Schieck, Elise G.
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Lartigue, C.
,
Frey, J.
,
Vozza, N.
,
Hegermann, J.
,
Miller, R.A.
,
Valguarnera, E.
,
Muriuki, C.
,
Meens, J.
,
Nene, Vishvanath
,
Naessens, Jan
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Weber, J.
,
Lowary, T.L.
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Vashee, S.
,
Feldman, M.F.
,
Jores, Joerg
,
[Galactofuranose in Mycoplasma mycoides is important for membrane integrity and conceals adhesins but does not contribute to serum resistance]
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Galactofuranose in Mycoplasma mycoides is important for membrane integrity and conceals adhesins but does not contribute to serum resistance
We have recently shown that the BoLA-A18 variant haplotype (BoLA-6*01302) is more prevalent than the BoLA-A18 haplotype (BoLA-6*01301) in a sample of Holstein/Friesian cattle in Kenya. These MHC class I allelic variants differ by a single amino acid polymorphism (Glu97 to Leu97) in the peptide-bi...
Svitek, Nicholas
,
Awino, Elias
,
Nene, Vishvanath
,
Steinaa, Lucilla
,
[BoLA-6*01301 and BoLA-6*01302, two allelic variants of the A18 haplotype, present the same epitope from the Tp1 antigen of Theileria parva]
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BoLA-6*01301 and BoLA-6*01302, two allelic variants of the A18 haplotype, present the same epitope from the Tp1 antigen of Theileria parva
Transcriptome analysis can provide useful data for refining genome sequence annotation. Application of massively parallel signature sequencing (MPSS) revealed reproducible transcription, in multiple MPSS cycles, from 73% of computationally predicted genes in the Theileria parva schizont lifecycle...
Shah, Tushaar
,
Villiers, Etienne P. de
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Nene, Vishvanath
,
Hass, B.
,
Taracha, E.A.
,
Gardner, J.M.
,
Sansom, C.
,
Pelle, Roger
,
Bishop, Richard P.
,
[Using the transcriptome to annotate the genome revisited: application of massively parallel signature sequencing (MPSS)]
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Using the transcriptome to annotate the genome revisited: application of massively parallel signature sequencing (MPSS)
We describe the generation of an auto-annotated index of genes that are expressed in the salivary glands of four-day fed female adult Rhipicephalus appendiculatus ticks. A total of 9162 EST sequences were derived from an uninfected tick cDNA library and 9844 ESTs were from a cDNA library from tic...
Nene, Vishvanath
,
Lee, D.
,
Kang'a, S.
,
Skilton, Robert A.
,
Shah, Tushaar
,
Villiers, Etienne P. de
,
Mwaura, S.
,
Taylor, D.
,
Quackenbush, J.
,
Bishop, Richard P.
,
[Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva]
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Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva
Reassociation kinetics and flow cytometry data indicate that ixodid tick genomes are large, relative to most arthropods, containing ≥ 109 base pairs. The molecular basis for this is unknown. We have identified a novel small interspersed element with features of a tRNA-derived SINE, designated Ruk...
Sunter, J.D.
,
Patel, S.P.
,
Skilton, Robert A.
,
Knowles, Donald P.
,
Scoles, Glen A.
,
Nene, Vishvanath
,
Villiers, Etienne P. de
,
Bishop, Richard P.
,
[A novel SINE family occurs frequently in both genomic DNA and transcribed sequences in ixodid ticks of the arthropod sub-phylum Chelicerata]
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A novel SINE family occurs frequently in both genomic DNA and transcribed sequences in ixodid ticks of the arthropod sub-phylum Chelicerata