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Journal Article 2000

Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)

Autor: Ouma, J.O. Masake, R.A. Masiga, Daniel K. Moloo, S.K. Njuguna, J.T. Ndung'u, J.M.

A visually read dot-enzyme linked immunosorbent asay (dot-ELISA) developed for the detection of trypanosomes in tsetse flies (Glossina spp.) was evaluated in the laboratory and under field conditions. In the evaluation, the fly dissection method was used as a standard technique and compared to th...

Enlace original: https://cgspace.cgiar.org/handle/10568/29977

Investigación en Agricultura

TRYPANOSOMIASIS

GLOSSINIDAE

ELISA

Citar Enlace permanente

Ouma, J.O. , Masake, R.A. , Masiga, Daniel K. , Moloo, S.K. , Njuguna, J.T. , Ndung'u, J.M. , [Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)] , Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)

Conference Paper 1999

Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)

Autor: Ouma, J.O. Masake, R.A. Masiga, Daniel K. Moloo, S.K. Minja, S.H. Njuguna, J.T. Olaho-Mukani, W. Ndung'u, J.M.

Dot-enzyme linked immunosorbent assay (dot-ELISA), developed for the detection and identification of trypanosome species in tsetse flies, was validated in the laboratory by comparing its sensitivity to that of the polymerase chain reaction (PCR). Tsetse flies were experimentally infected with dif...

Enlace original: https://cgspace.cgiar.org/handle/10568/50294

Investigación en Agricultura

TRYPANOSOMIASIS

GLOSSINIDAE

ELISA

Citar Enlace permanente

Ouma, J.O. , Masake, R.A. , Masiga, Daniel K. , Moloo, S.K. , Minja, S.H. , Njuguna, J.T. , Olaho-Mukani, W. , Ndung'u, J.M. , [Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)] , Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)

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Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)

Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)