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Several species of double-stranded RNA (dsRNA) were found to occur in the cassava clone Secundina, which was thought to be virus- free. A large dsRNA molecule (6.6 x 106 Mr = relative molecular mass)(L-dsRNA) was shown to be graft, but not mechanically transmitted and occurred primarily in a nucl...


Gabriel, C.J.WALSH, R.Nolt, Barry L.[Evidence for a latent viruslike agent in cassava]Evidence for a latent viruslike agent in cassava
Roa E, Julio CésarMafla, G.Roca, William M.Pineda López, BenjamínNolt, Barry L.[Elimination of cassava (Manihot esculenta Crantz) viruses by thermotherapy and meristem-tip culture]Elimination of cassava (Manihot esculenta Crantz) viruses by thermotherapy and meristem-tip culture
Nolt, Barry L.Pineda López, BenjamínVelasco, Ana Cecilia[Surveys of cassava plantations in Colombia for virus and virus- like diseases]Surveys of cassava plantations in Colombia for virus and virus- like diseases

Large quantities of cassava common mosaic virus (CCMV) were purified from systemically infected Nicotiana benthamiana plants. A polyclonal antiserum, with a titre of 1/128 in the tube precipitin test, was produced by immunising rabbits with purified virus. Viral antigens were detected in cassava,...


Nolt, Barry L.Velasco, Ana CeciliaPineda López, Benjamín[Improved purification procedure and some serological and physical properties of cassava common mosaic virus from South America]Improved purification procedure and some serological and physical properties of cassava common mosaic virus from South America
Roca, William M.Szabados, LaszloMafla, G.Roa E, Julio CésarNolt, Barry L.[Virus elimination and clonal propagation of cassava (Manihot esculenta Crantz)]Virus elimination and clonal propagation of cassava (Manihot esculenta Crantz)
Nolt, Barry L.Pineda López, BenjamínVelasco, Ana Cecilia[Avances sobre identificación, detección y manejo de los virus de la yuca en América Latina]Avances sobre identificación, detección y manejo de los virus de la yuca en América Latina

Several species of double-stranded RNA (dsRNA) were found to occur in the cassava clone Secundina, which was thought to be virus- free. A large dsRNA molecule (6.6 x 106 Mr = relative molecular mass)(L-dsRNA) was shown to be graft, but not mechanically transmitted and occurred primarily in a nucl...


Gabriel, C.J.Walsh, RNolt, Barry L.[Postharvest root deterioration or vascular streaking of cassava clones (accessions and breeding lines) was evaluated with harvests at different ages (8 and 12 mo.), seasons (beginning and end of wet season), and locations (CIAT-Palmira, Carimagua, and Caribia in Colombia) to assess the effect of environmental factors on cassava genotypes. Vascular streaking was sensitive to any of these environmental factors. Genotype x location interaction was highly significant suggesting that final cv. selection should be carried out in each location. Genetic analysis carried out in one location suggested that inheritance of vascular streaking was, at least partly, controlled by additive factors and was quantitative rather than qualitative. Narrow sense heritability of 0.64 was obtained. Vascular streaking was highly and significantly correlated with root DM content in all the trials conducted during 7 yr. Since free recombination between resistance to vascular streaking and high root DM content may not be possible, the most acceptable balance between these 2 traits should be defined according to the use of product and edaphoclimatic condition of production area prior to selection program. (AS)]Postharvest root deterioration or vascular streaking of cassava clones (accessions and breeding lines) was evaluated with harvests at different ages (8 and 12 mo.), seasons (beginning and end of wet season), and locations (CIAT-Palmira, Carimagua, and Caribia in Colombia) to assess the effect of environmental factors on cassava genotypes. Vascular streaking was sensitive to any of these environmental factors. Genotype x location interaction was highly significant suggesting that final cv. selection should be carried out in each location. Genetic analysis carried out in one location suggested that inheritance of vascular streaking was, at least partly, controlled by additive factors and was quantitative rather than qualitative. Narrow sense heritability of 0.64 was obtained. Vascular streaking was highly and significantly correlated with root DM content in all the trials conducted during 7 yr. Since free recombination between resistance to vascular streaking and high root DM content may not be possible, the most acceptable balance between these 2 traits should be defined according to the use of product and edaphoclimatic condition of production area prior to selection program. (AS)

Several techniques used to eliminate viruses from cassava are described, namely heat therapy, apical tissue culture, and micropropagation. Virological tests to index cassava viruses are also indicated as follows: ELISA, dsRNA, mechanical inoculation of leaf sap in plants on Chenopodium quinoa, an...


Roca, William M.Nolt, Barry L.Mafla, G.Roa E, J.C.Reyes, R.[Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz)]Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz)

Con el proposito de obtener metodos de diagnostico rapido del virus del mosaico comun de la yuca (CCMV) que faciliten el intercambio de germoplasma de yuca (Manihot esculenta Crantz) con menores riesgos cuarentenarios, se compararon tres variaciones de la tecnica ELISA: la del doble emparedado (D...


Velasco, Ana CeciliaNolt, Barry L.Pineda López, Benjamín[Comparacion de tres metodos de la tecnica inmunoenzimatica ELISA para el diagnostico del virus del mosaico comun de la yuca]Comparacion de tres metodos de la tecnica inmunoenzimatica ELISA para el diagnostico del virus del mosaico comun de la yuca

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