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Nolt, Barry L.Pineda López, BenjamínVelasco, Ana Cecilia[Surveys of cassava plantations in Colombia for virus and virus- like diseases]Surveys of cassava plantations in Colombia for virus and virus- like diseases

Large quantities of cassava common mosaic virus (CCMV) were purified from systemically infected Nicotiana benthamiana plants. A polyclonal antiserum, with a titre of 1/128 in the tube precipitin test, was produced by immunising rabbits with purified virus. Viral antigens were detected in cassava,...


Nolt, Barry L.Velasco, Ana CeciliaPineda López, Benjamín[Improved purification procedure and some serological and physical properties of cassava common mosaic virus from South America]Improved purification procedure and some serological and physical properties of cassava common mosaic virus from South America

Several techniques used to eliminate viruses from cassava are described, namely heat therapy, apical tissue culture, and micropropagation. Virological tests to index cassava viruses are also indicated as follows: ELISA, dsRNA, mechanical inoculation of leaf sap in plants on Chenopodium quinoa, an...


Roca, William M.Nolt, Barry L.Mafla, G.Roa E, J.C.Reyes, R.[Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz)]Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz)

Con el proposito de obtener metodos de diagnostico rapido del virus del mosaico comun de la yuca (CCMV) que faciliten el intercambio de germoplasma de yuca (Manihot esculenta Crantz) con menores riesgos cuarentenarios, se compararon tres variaciones de la tecnica ELISA: la del doble emparedado (D...


Velasco, Ana CeciliaNolt, Barry L.Pineda López, Benjamín[Comparacion de tres metodos de la tecnica inmunoenzimatica ELISA para el diagnostico del virus del mosaico comun de la yuca]Comparacion de tres metodos de la tecnica inmunoenzimatica ELISA para el diagnostico del virus del mosaico comun de la yuca

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