Transcriptome analysis can provide useful data for refining genome sequence annotation. Application of massively parallel signature sequencing (MPSS) revealed reproducible transcription, in multiple MPSS cycles, from 73% of computationally predicted genes in the Theileria parva schizont lifecycle...
Shah, Tushaar
,
Villiers, Etienne P. de
,
Nene, Vishvanath
,
Hass, B.
,
Taracha, E.A.
,
Gardner, J.M.
,
Sansom, C.
,
Pelle, Roger
,
Bishop, Richard P.
,
[Using the transcriptome to annotate the genome revisited: application of massively parallel signature sequencing (MPSS)]
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Using the transcriptome to annotate the genome revisited: application of massively parallel signature sequencing (MPSS)
East Coast fever, caused by the tick-borne intracellular apicomplexan parasite Theileria parva, is a highly fatal lymphoproliferative disease of cattle. The pathogenic schizont-induced lymphocyte transformation is a unique cancer-like condition that is reversible with parasite removal. Schizont-i...
Graham, S.P.
,
Pelle, Roger
,
Honda, Y.
,
Mwangi, D.M.
,
Tonukari, N.J.
,
Yamage, M.
,
Glew, E.J.
,
Villiers, Etienne P. de
,
[Theileria parva candidate vaccine antigens recognized by immune bovine cytotoxic T lymphocytes]
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Theileria parva candidate vaccine antigens recognized by immune bovine cytotoxic T lymphocytes
Silatsa, Barberine A.
,
Kuiate, Jules-Roger
,
Njiokou, F.
,
Simo, G.
,
Feussom, J.-M.K.
,
Tunrayo, A.
,
Amzati, Gaston S.
,
Bett, Bernard K.
,
Bishop, R.
,
Githaka, Naftaly W.
,
Opiyo, S.O.
,
Djikeng, Appolinaire
,
Pelle, Roger
,
[A countrywide molecular survey leads to a seminal identification of the invasive cattle tick Rhipicephalus (Boophilus) microplus in Cameroon, a decade after it was reported in Cote d’Ivoire]
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A countrywide molecular survey leads to a seminal identification of the invasive cattle tick Rhipicephalus (Boophilus) microplus in Cameroon, a decade after it was reported in Cote d’Ivoire
Immunity against the bovine protozoan parasite Theileria parva has previously been shown to be mediated through lysis of parasite-infected cells by MHC class I restricted CD8+ cytotoxic T lymphocytes. It is hypothesized that identification of CTL target schizont antigens will aid the development ...
Graham, S.P.
,
Honda, Y.
,
Pelle, Roger
,
Mwangi, D.M.
,
Glew, E.J.
,
Villiers, Etienne P. de
,
Shah, Tushaar
,
Bishop, Richard P.
,
Bruggen, P. van der
,
Nene, Vishvanath
,
Taracha, E.L.N.
,
[A novel strategy for the identification of antigens that are recognised by bovine MHC class I restricted cytotoxic T cells in a protozoan infection using reverse vaccinology]
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A novel strategy for the identification of antigens that are recognised by bovine MHC class I restricted cytotoxic T cells in a protozoan infection using reverse vaccinology
A Trypanosoma brucei brucei DNA repeat sequence termed NlaIII repeat (NR) was originally isolated from a multidrug-resistant field isolate CP547 [Jamnadass, R., 1995. Identification and characterisation of an extrachromosomal element from a multidrug-resistant isolate of T. brucei brucei, Ph.D. t...
Jamnadass, Ramni H.
,
Pelle, Roger
,
Pandit, P.
,
Ricard, B.
,
Murphy, N.B.
,
[Trypanosoma brucei: Composition, organisation, plasticity, and differential transcription of NlaIII repeat elements in drug-resistant and sensitive isolates]
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Trypanosoma brucei: Composition, organisation, plasticity, and differential transcription of NlaIII repeat elements in drug-resistant and sensitive isolates
The expression and characterization of a recombinant antigen termed Ov-47 are described. Ov-47 was identified and isolated from a lambda gt-11 eDNA expression library derived from adult female Onclrucercu volvukrs mRNA using rabbit antiserum raised against the surface proteins of O. vulvulcw fema...
Ghoyomu, S.M.
,
Souopgui, J.
,
Pelle, Roger
,
Titanji, V.P.K.
,
[Expression and characterization of Ov-47, a dominant antigen of Onchocerca volvulus]
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Expression and characterization of Ov-47, a dominant antigen of Onchocerca volvulus
Awino, Elias
,
Aye, R.
,
Bertrand, S.
,
Gesharisha, J.
,
Gicheru, N.
,
Jores, Joerg
,
Liljander, Anne M.
,
Muriuki, C.
,
Naessens, Jan
,
Nene, Vishvanath
,
Njoroge, T.
,
Nyongesa, S.
,
Pelle, Roger
,
Ssajjakambwe, P.
,
Saya, R.
,
Schieck, Elise G.
,
Steinaa, Lucilla
,
Svitek, Nicholas
,
Wasilwa, J.
,
[ILRI vaccine biosciences—Planting the orchard: (ILRI’s Vaccine Platform‐ILVAC)]
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ILRI vaccine biosciences—Planting the orchard: (ILRI’s Vaccine Platform‐ILVAC)
Masake, R.A.
,
Nantulya, V.M.
,
Pelle, Roger
,
Makau, J.M.
,
Gathuo, H.K.W.
,
Ole-MoiYoi, O.K.
,
[A species-specific antigen of Trypanosoma (Duttonella) vivax detectable in the course of infection is encoded by a differentially-expressed tandemly reiterated gene]
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A species-specific antigen of Trypanosoma (Duttonella) vivax detectable in the course of infection is encoded by a differentially-expressed tandemly reiterated gene
Objective: To determine the role of an immunodominant antigen OvPG-1 in human onchocerciasis. Design: Cross-sectional study of subjects living in three onchocerciasis endemic areas. Setting: Mbonge and Tubah divisions of Western Cameroon and in Esmeralda Province of Ecuador. Subjects: There were ...