The genetic diversity and phylogenetic relationships of 29 East African highland banana (Musa spp.) cultivars and two outgroup taxa, M. acuminata Calcutta 4 and Agbagba were surveyed by RAPD analysis. A genetic similarity matrix was established based on the presence or absence of polymorphic ampl...
Pillay, M.
,
Ogundiwin, E.
,
Nwakanma, D.
,
Ude, G.
,
Tenkouano, A.
,
[Analysis of genetic diversity and relationships in East African banana germplasm]
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Analysis of genetic diversity and relationships in East African banana germplasm
Genetic diversity and relationships were assessed in 28 accessions of Musa acuminata (AA) Colla and Musa balbisiana (BB) Colla, and some of their natural hybrids, using the amplified fragment length poly-morphisms (AFLP) technique. Fifteen AFLP +3 primer pairs produced 527 polymorphic bands among...
Ude, G.
,
Pillay, M.
,
Nwakanma, D.
,
Tenkouano, A.
,
[Genetic diversity in Musa acuminata Colla and Musa balbisiana Colla and some of their natural hybrids using AFLP markers]
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Genetic diversity in Musa acuminata Colla and Musa balbisiana Colla and some of their natural hybrids using AFLP markers
Banana is an important food and cash crop in Uganda. The crop displays wide diversity but the genetic relationship between and withing cultivars is largely unknown. A study was conducted to assess genetic relationships among 'Sukali Ndizi' clones collected from 16 different localities in Uganda ...
Pillay, M.
,
Dimkpa, C.O.
,
Ude, G.
,
Makumbi, Dan
,
Tushemereirwe, W.K.
,
[Genetic variation in the banana cultivar Sukali ndizi grown in different regions of Uganda]
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Genetic variation in the banana cultivar Sukali ndizi grown in different regions of Uganda
Blomme, Guy
,
Ploetz, R.
,
Jones, D.
,
Langhe, E. de
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Price, N.
,
Gold, C.
,
Geering, A.
,
Viljoen, A.
,
Karamura, D.
,
Pillay, M.
,
Tinzaara, W.
,
Teycheney, P.Y.
,
Lepoint, P.
,
Karamura, E.B.
,
Buddenhagen, I.W.
,
[A historical overview of the appearance and spread of Musa pests and pathogens on the African continent: highlighting the importance of clean Musa planting materials and quarantine measures]
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A historical overview of the appearance and spread of Musa pests and pathogens on the African continent: highlighting the importance of clean Musa planting materials and quarantine measures
Africa is one of the secondary centers of diversity for the genus Musa. There is a wide range of genetic diversity in the bananas found in the continent with different types dominating different ecological sub-regions. Banana production in Africa is affected by a number of diseases and pests. The...
Pillay, M.
,
Ssebuliba, R.
,
Hartman, J.
,
Vuylsteke, D.R.
,
Talengera, D.
,
Tushemereirwe, W.K.
,
[Conventional breeding strategies to enhance the sustainability of Musa biodiversity conservation for endemic cultivars on farm]
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Conventional breeding strategies to enhance the sustainability of Musa biodiversity conservation for endemic cultivars on farm
The East African highland banana (Musa spp. AAA) is an economically important food crop. The crop is affected by a number of diseases and pests. Genetic improvement of the crop is hindered by very low seed set and poor seed germination. The objective of this study was to compare seed set, seed qu...
Ssebuliba, R.
,
Talengera, D.
,
Makumbi, Dan
,
Namanya, P.
,
Tenkouano, A.
,
Tushemereirwe, W.K.
,
Pillay, M.
,
[Reproductive efficiency and breeding potential of East African highland (Musa AAAEA) bananas]
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Reproductive efficiency and breeding potential of East African highland (Musa AAAEA) bananas
Musa acuminata Colla (AA genomes) and Musa balbisiana Colla (BB genomes) are the diploid ancestors of modern bananas that are mostly diploid or triploid cultivars with various combinations of the A and B genomes, including AA, AAA, BB, AAB and ABB. The objective of this study was to identify mole...
Nwakanma, D.C.
,
Pillay, M.
,
Okoli, B.E.
,
Tenkouano, A.
,
[PCRRFLP of the ribosomal DNA internal transcribed spacers (ITS) provides markers for the A and B genomes in Musa L.]
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PCRRFLP of the ribosomal DNA internal transcribed spacers (ITS) provides markers for the A and B genomes in Musa L.
The objective of this study was to construct a molecular phylogeny of the genus Musa using restriction-site polymorphisms of the chloroplast (cpDNA) and mitochondrial DNA (mtDNA). Six cpDNA and two mtDNA sequences were amplified individually in polymerase chain reaction (PCR) experiments in 13 sp...
Nwakanma, D.C.
,
Pillay, M.
,
Okoli, B.E.
,
Tenkouano, A.
,
[Sectional relationships in the genus Musa L. inferred from PCRRFLP of organelle DNA sequences]
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Sectional relationships in the genus Musa L. inferred from PCRRFLP of organelle DNA sequences
The AFLP technique was used to assess the genetic diversity and sectional relationships in 39 accessions representing the four main sections of the genus Musa. Eight AFLP + 3 primer pairs produced 260 poly-morphic bands that were used in cluster and PCO analysis. A wide range of variability was o...
Ude, G.
,
Pillay, M.
,
Nwakanma, D.
,
Tenkouano, A.
,
[Analysis of genetic diversity and sectional relationships in Musa using AFLP markers]
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Analysis of genetic diversity and sectional relationships in Musa using AFLP markers
Cytological studies in Musa spp. L. are hampered by the small size and poor staining ability of the chromosomes. The aim of this experiment was to use silver nitrate as a staining agent for Musa chromosomes. The results of this study showed that silver nitrate is a useful alternative stain for cy...