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Article

Autor: Figueroa Villalobos, Elías Valdebenito Isler, Iván Merino, O. Ubilla Madrid, Andrea Risopatron, J. Farias, J. G.

The objective of this study was to determine the effect of freezing on the function in Atlantic salmon Salmo salar spermatozoa. The semen was frozen in Cortland's medium+1.3M dimethyl sulphoxide+0.3M glucose+2% bovine serum albumin (final concentration) in a ratio of 1:3 (semen:cryoprotectant) as...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/2909

Repositorio Digital Universidad Católica de Temuco
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Figueroa Villalobos, Elías ,  Valdebenito Isler, Iván ,  Merino, O. ,  Ubilla Madrid, Andrea ,  Risopatron, J. ,  Farias, J. G. ,  [Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology] ,  Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology
Article

Autor: Risopatron, J. Merino, O. Cheuqueman, C. Figueroa Villalobos, Elías Sanchez, R. Farias, J. G. Valdebenito Isler, Iván

The knowledge of sperm quality in the broodstock males of different ages is a prerequisite to identify the reproductive ability of cultivated fish for the hatchery management. Thus, in this work, we analysed sperm function of the semen stored of broodstock males of rainbow trout (Oncorhychus myki...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/2944

Repositorio Digital Universidad Católica de Temuco
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Risopatron, J. ,  Merino, O. ,  Cheuqueman, C. ,  Figueroa Villalobos, Elías ,  Sanchez, R. ,  Farias, J. G. ,  Valdebenito Isler, Iván ,  [Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)] ,  Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)
Artículo de Revista

Autor: Figueroa Villalobos, Elías Farias, J.G. Lee Estevez, M. Valdebenito Isler, Iván Risopatron, J. Magnotti, C. Romero, J. Watanabe, I. Oliveira, R.P.S.

The use of α-tocopherol and ascorbic acid in freezing medium prevents lipoperoxidation and damage by ROS (reactive oxygen species); however, the effects of these antioxidants in salmonid spermatozoa have not been studied. In this work the protective effects of antioxidants on the enzymatic activi...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/2260

Repositorio Digital Universidad Católica de Temuco
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Figueroa Villalobos, Elías ,  Farias, J.G. ,  Lee Estevez, M. ,  Valdebenito Isler, Iván ,  Risopatron, J. ,  Magnotti, C. ,  Romero, J. ,  Watanabe, I. ,  Oliveira, R.P.S. ,  [Sperm cryopreservation with supplementation of a-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)] ,  Sperm cryopreservation with supplementation of a-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)
Article

Autor: Ubilla Madrid, Andrea Valdebenito Isler, Iván Arias, M. E. Risopatron, J.

In vitro storage of salmonid eggs leads to aging of the cells causing a decline in quality and reducing their capacity to develop and produce embryos. The quality of salmonid embryos is assessed by morphologic analyses; however, data on the application of biomarkers to determine the cell viabilit...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3012

Repositorio Digital Universidad Católica de Temuco
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Ubilla Madrid, Andrea ,  Valdebenito Isler, Iván ,  Arias, M. E. ,  Risopatron, J. ,  [Viability and DNA fragmentation of rainbow trout embryos (Oncorhynchus mykiss) obtained from eggs stored at 4 degrees C] ,  Viability and DNA fragmentation of rainbow trout embryos (Oncorhynchus mykiss) obtained from eggs stored at 4 degrees C
Article

Autor: Figueroa Villalobos, Elías Risopatron, J. Sanchez, R. Isachenko, E. Merino, O. Isachenko, V. Valdebenito Isler, Iván

This study was designed to test a vitrification method on spermatozoa of sex-reversed rainbow trout and determine the ability of sucrose, dimethyl sulfoxide (DMSO), bovine serum albumin (BSA) and seminal plasma (SP) to protect these cells from cryoinjuries. The vitrification medium comprised a st...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3310

Repositorio Digital Universidad Católica de Temuco
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Figueroa Villalobos, Elías ,  Risopatron, J. ,  Sanchez, R. ,  Isachenko, E. ,  Merino, O. ,  Isachenko, V. ,  Valdebenito Isler, Iván ,  [Spermatozoa vitrification of sex-reversed rainbow trout (Oncorhynchus mykiss): Effect of seminal plasma on physiological parameters] ,  Spermatozoa vitrification of sex-reversed rainbow trout (Oncorhynchus mykiss): Effect of seminal plasma on physiological parameters
Article

Autor: Merino, O. Sanchez, R. Risopatron, J. Isachenko, E. Katkov, I. I. Figueroa Villalobos, Elías Valdebenito Isler, Iván Mallmann, P. Isachenko, V.

The aims of this investigation were to test a novel technology comprising cryoprotectant-free vitrification of the spermatozoa of rainbow trout and to study the ability of sucrose and components of seminal plasma to protect these cells from cryo-injuries. Spermatozoa were isolated and vitrified u...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3644

Repositorio Digital Universidad Católica de Temuco
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Merino, O. ,  Sanchez, R. ,  Risopatron, J. ,  Isachenko, E. ,  Katkov, I. I. ,  Figueroa Villalobos, Elías ,  Valdebenito Isler, Iván ,  Mallmann, P. ,  Isachenko, V. ,  [Cryoprotectant-free vitrification of fish (Oncorhynchus mykiss) spermatozoa: first report] ,  Cryoprotectant-free vitrification of fish (Oncorhynchus mykiss) spermatozoa: first report
Article

Autor: Valdebenito Isler, Iván Cosson, J. Contreras, P. Sanchez, J. C. Oliveira, R. P. S. Risopatron, J. Farias, J. G. Figueroa Villalobos, Elías

Spermatological research of the Patagonian blennie was carried, specifically biometric parameters, sperm density, sperm count and motility in different activation mediums (815, 716, 590 and 0mOSmkg(-1)), at different temperatures (5, 10 and 15 degrees C) and pH levels (5, 7 and 9). The results in...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3935

Repositorio Digital Universidad Católica de Temuco
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Valdebenito Isler, Iván ,  Cosson, J. ,  Contreras, P. ,  Sanchez, J. C. ,  Oliveira, R. P. S. ,  Risopatron, J. ,  Farias, J. G. ,  Figueroa Villalobos, Elías ,  [Spermatological research of experimentally farmed Patagonian blenny (Eleginops maclovinus) (Perciformes: Eleginopsidae) in Chile] ,  Spermatological research of experimentally farmed Patagonian blenny (Eleginops maclovinus) (Perciformes: Eleginopsidae) in Chile
Article

Autor: Merino, O. Risopatron, J. Sanchez, R. Isachenko, E. Figueroa Villalobos, Elías Valdebenito Isler, Iván Isachenko, V.

The aim of the present investigations was to test a novel technology comprising cryoprotectant-free vitrification of the spermatozoa of rainbow trout and to study the ability of sucrose and components of seminal plasma to protect these cells from cryoinjuries. Spermatozoa were isolated and vitrif...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3378

Repositorio Digital Universidad Católica de Temuco
Enlace permanente
Merino, O. ,  Risopatron, J. ,  Sanchez, R. ,  Isachenko, E. ,  Figueroa Villalobos, Elías ,  Valdebenito Isler, Iván ,  Isachenko, V. ,  [Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness] ,  Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness
Abstract

Autor: Figueroa Villalobos, Elías Lee Estevez, M. Risopatron, J. Valdebenito Isler, Iván Pinheiro de Souza, R. Farias, J. G.
Enlace original: http://repositoriodigital.uct.cl/handle/10925/3492

Repositorio Digital Universidad Católica de Temuco
Enlace permanente
Figueroa Villalobos, Elías ,  Lee Estevez, M. ,  Risopatron, J. ,  Valdebenito Isler, Iván ,  Pinheiro de Souza, R. ,  Farias, J. G. ,  [EFFECTS OF CRYOPRESERVATION ON OXIDATIVE STRESS AND ANTIOXIDANT ENZYME ACTIVITY IN SPERMATOZOA OF ATLANTIC SALMON (Salmo salar)] ,  EFFECTS OF CRYOPRESERVATION ON OXIDATIVE STRESS AND ANTIOXIDANT ENZYME ACTIVITY IN SPERMATOZOA OF ATLANTIC SALMON (Salmo salar)
Article

Autor: Figueroa Villalobos, Elías Farias, J. G. Lee Estevez, M. Valdebenito Isler, Iván Risopatron, J. Magnotti, C. Romero, J. Watanabe, I Oliveira, R. P. S.

The use of alpha-tocopherol and ascorbic acid in freezing medium prevents lipoperoxidation and damage by ROS (reactive oxygen species); however, the effects of these antioxidants in salmonid spermatozoa have not been studied. In this work the protective effects of antioxidants on the enzymatic ac...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3926

Repositorio Digital Universidad Católica de Temuco
Enlace permanente
Figueroa Villalobos, Elías ,  Farias, J. G. ,  Lee Estevez, M. ,  Valdebenito Isler, Iván ,  Risopatron, J. ,  Magnotti, C. ,  Romero, J. ,  Watanabe, I ,  Oliveira, R. P. S. ,  [Sperm cryopreservation with supplementation of alpha-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)] ,  Sperm cryopreservation with supplementation of alpha-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)

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