A procedure based on acid polyacrylamide gel electrophoresis (acid PAGE) has been described. Resolution of phaseolin bands appears to be relatively superior to that obtained with basic PAGE system. The procedure discriminates S, T, C, M and B types of phaseolins; S, T and C have been reported pre...
Hussain, A.
,
Espinel, MT
,
Roca, William M.
,
Bushhuk, W
,
[Acid PAGE procedure for detection of phaseolins in field beans (Phaseolus vulgaris L.)]
,
Acid PAGE procedure for detection of phaseolins in field beans (Phaseolus vulgaris L.)
Singh, Shree P.
,
Debouck, Daniel G.
,
Roca, William M.
,
[Interspecific hybridization between Phaseolus vulgaris L. and P. parvifolius Freytag]
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Interspecific hybridization between Phaseolus vulgaris L. and P. parvifolius Freytag
Singh, Shree P.
,
Debouck, Daniel G.
,
Roca, William M.
,
[Broadening the genetic base of common bean cultivars]
,
Broadening the genetic base of common bean cultivars
The value of intra- and interracial populations in common bean (Phaseolus vulgaris L.) needs to be determined in order to create useful genetic variation for maximizing gains from selection, broadening the genetic base of commercial cultivars, and making efficient use of available resources. Five...
Welsh, W
,
Bushuk, W
,
Roca, William M.
,
Singh, Shree P.
,
[Characterization of agronomic traits and markers of recombinant inbred lines from intra-and interracial populations of Phaseolus vulgaris L.]
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Characterization of agronomic traits and markers of recombinant inbred lines from intra-and interracial populations of Phaseolus vulgaris L.
Hoyos, R.A.
,
Roca, William M.
,
Hosfield, GL
,
Mayer, Jorge Edgard
,
[Particle gun transient expression of sexual embryos of common bean]
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Particle gun transient expression of sexual embryos of common bean
Singh, Shree P.
,
Debouck, Daniel G.
,
Roca, William M.
,
[Successful interspecific hybridization between Phaseolus vulgaris L. and P. costaricensis Freytag & Debouck]
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Successful interspecific hybridization between Phaseolus vulgaris L. and P. costaricensis Freytag & Debouck
Muñoz Flórez, Ligia Carmenza
,
Blair, Matthew W.
,
Duque E., Myriam Cristina
,
Roca, William M.
,
Tohme, Joseph M.
,
[Level of introgression in inter-specific (Phaseolus vulgaris x P. acutifolius) congruity-backcross lines]
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Level of introgression in inter-specific (Phaseolus vulgaris x P. acutifolius) congruity-backcross lines
Los trabajos realizados en varias especies de plantas revelan que una porción significativa de los genes que se expresan en el esporofito también lo hacen en el grano de polen, en consecuencia la presión de selección a nivel de polen debe conducir a modificaciones en caracteres cualitativos o cu...
Vásquez, L.M.
,
Montes Rojas, C
,
White, Jeffrey W.
,
Roca, William M.
,
[Metodología para la selección de líneas de fríjol Phaseolus vulgaris L. por estrés de temperatura a nivel microgametofítico]
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Metodología para la selección de líneas de fríjol Phaseolus vulgaris L. por estrés de temperatura a nivel microgametofítico
Se construyó una pequeña librería de cDNA de fríjol (phaseolus vulgaris L.) de alrededor de 1500 clones, con el fin de incrementar la saturación del mapa de ligamiento de fríjol con marcadores de RFLPs. Para la generación de la librería se utilizó la técnica de amplificación por PCR (Jepson et al...
Ramírez, H.
,
Mayer, Jorge Edgard
,
Roca, William M.
,
Tohme, Joseph M.
,
López, Y.
,
[Construcción de una genoteca de cDNA de fríjol (Phaseolus vulgaris L.) para mapeo genético]
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Construcción de una genoteca de cDNA de fríjol (Phaseolus vulgaris L.) para mapeo genético
Congruity and recurrent backcross interspecific hybrids between common bean (Phaseolus vulgaris L.) and tepary bean (P. acutifolius A. Gray) were compared for the amount of introgression occurring between genomes by amplified fragment length polymorphism (AFLP) markers. A total of 60 genotypes we...
Muñoz Flórez, Ligia Carmenza
,
Blair, Matthew W.
,
Duque E., Myriam Cristina
,
Tohme, Joseph M.
,
Roca, William M.
,
[Introgression in common bean x tepary bean interspecific congruity-backcross line as measured by AFLP markers]
,
Introgression in common bean x tepary bean interspecific congruity-backcross line as measured by AFLP markers