Bacteriophage clones containing Theileria parva genomic DNA derived from two different telomeres were isolated and the nucleotide sequences of the telomeric repeats and adjacent telomere-associated (TAS) DNA were determined. The T. parva telomeric repeat sequences, a tandem array of TTTTAGGG or T...
Sohanpal, B.K.
,
Morzaria, S.P.
,
Gobright, E.I.
,
Bishop, Richard P.
,
[Characterisation of the telomeres at opposite ends of a 3 Mb Theileria parva chromosome]
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Characterisation of the telomeres at opposite ends of a 3 Mb Theileria parva chromosome
Telomere-associated (TA) DNA sequences of the intracellular protozoan parasite Theileria parva were isolated by a novel strategy using a modified version of single-specific-primer polymerase chain reaction (SSP-PCR). Nucleotide sequences of non-coding TA DNA from three telomeres (6017 bp, 2435 bp...
Sohanpal, B.K.
,
Bishop, Richard P.
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Wasawo, D.
,
[Cloning of telomere-associated DNA using single-specific-primer polymerase chain reaction provides evidence for a conserved sequence directly adjacent to Theileria parva telomeric repeats]
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Cloning of telomere-associated DNA using single-specific-primer polymerase chain reaction provides evidence for a conserved sequence directly adjacent to Theileria parva telomeric repeats
Bishop, Richard P.
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Sohanpal, B.K.
,
Kariuki, D.
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Young, A.S.
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Nene, Vishvanath
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Baylis, H.
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Allsopp, B.A.
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Spooner, P.R.
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Dolan, T.T.
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Morzaria, S.P.
,
[Detection of a carrier state in Theileria parva infected cattle using the polymerase chain reaction]
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Detection of a carrier state in Theileria parva infected cattle using the polymerase chain reaction
Conrad, P.A.
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Baldwin, C.L.
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Brown, W.C.
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Sohanpal, B.K.
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Dolan, T.T.
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Goddeeris, B.M.
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Demartini, J.C.
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Ole-MoiYoi, O.K.
,
[Infection of bovine T cell clones with genotypically distinct Theileria parva parasites and analysis of their cell surface phenotype]
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Infection of bovine T cell clones with genotypically distinct Theileria parva parasites and analysis of their cell surface phenotype
A cloned Theileria parva telomeric DNA sequence, designated pTpUtel, was used to characterize T. parva stocks and clones by hybridization to EcoRI-digested DNA. Eight of the nine T. parva stocks tested were discriminated by the telomeric restriction-fragment-length polymorphisms (RFLOs). Two isol...
Bishop, Richard P.
,
Spooner, P.R.
,
Sohanpal, B.K.
,
[Genetic fingerprinting of Theileria parva using a telomeric DNA probe]
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Genetic fingerprinting of Theileria parva using a telomeric DNA probe
Polymorphic, multicopy gene families are frequently located in subtelomeric regions of the genomes of parasitic protozoa. Theileria parva telomere-associated (TA) DNA from two chromosomes contained long open reading frames (ORFs) 54% identical at the N-termini whose ends were 2670 and 2680 bp fro...
Bishop, Richard P.
,
Gobright, E.I.
,
Nene, Vishvanath
,
Morzaria, S.P.
,
Musoke, A.J.
,
Sohanpal, B.K.
,
[Polymorphic open reading frames encoding secretory proteins are located less than 3 kilobases from Theileria parva telomeres]
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Polymorphic open reading frames encoding secretory proteins are located less than 3 kilobases from Theileria parva telomeres
Concerted evolution of multicopy gene families in vertebrates is recognized as an important force in the generation of biological novelty but has not been documented for the multicopy genes of protozoa. A multicopy locus, Tpr, which consists of tandemly arrayed open reading frames (ORFs) containi...
Bishop, Richard P.
,
Musoke, A.J.
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Morzaria, S.P.
,
Sohanpal, B.K.
,
Gobright, E.I.
,
[Concerted evolution at a multicopy locus in the protozoan parasite Theileria parva: Extreme divergence of potential protein-coding sequences]
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Concerted evolution at a multicopy locus in the protozoan parasite Theileria parva: Extreme divergence of potential protein-coding sequences
The nucleotide sequences of the large subunit ribosomal RNA coding genes within the two single copy ribosomal DNA transcription units of a cloned Theileria parva isolate from a buffalo were determined. The two LSU rRNA coding units differed by 11 nucleotide substitutions and two deletions of 1 an...
Bishop, Richard P.
,
Gobright, E.I.
,
Spooner, P.R.
,
Allsopp, B.A.
,
Sohanpal, B.K.
,
Collins, N.
,
[Microsequence heterogeneity and expression of LSU rRNA genes within the two single copy ribosomal transcription units of Theileria parva]
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Microsequence heterogeneity and expression of LSU rRNA genes within the two single copy ribosomal transcription units of Theileria parva
Bishop, Richard P.
,
Sohanpal, B.K.
,
Morzaria, S.P.
,
Dolan, T.T.
,
Mwakima, F.N.
,
Young, A.S.
,
[Discrimination between Theileria parva and Theileria taurotragi in the salivary glands of Rhipicephalus appendiculatus ticks using oligonucleotides homologous to ribosomal RNA sequences]
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Discrimination between Theileria parva and Theileria taurotragi in the salivary glands of Rhipicephalus appendiculatus ticks using oligonucleotides homologous to ribosomal RNA sequences