Theileria parva causes an acute fatal disease in cattle, but infections are asymptomatic in the African buffalo (Syncerus caffer). Cattle can be immunized against the parasite by infection and treatment, but immunity is partially strain specific. Available data indicate that CD8+ T lymphocyte res...
Pelle, Roger
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Graham, S.P.
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Njahira, Moses N.
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Osaso, J.
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Saya, R.M.
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Odongo, David O.
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Toye, Philip G.
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Spooner, P.R.
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Musoke, A.J.
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Mwangi, D.M.
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Taracha, E.L.N.
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Morrison, W. Ivan
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Weir, W.
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Silva, J.
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Bishop, Richard P.
,
[Two Theileria parva CD8 T Cell Antigen genes are more variable in buffalo than cattle parasites, but differ in pattern of sequence diversity]
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Two Theileria parva CD8 T Cell Antigen genes are more variable in buffalo than cattle parasites, but differ in pattern of sequence diversity
African Cape buffalo (Syncerus caffer) is the wildlife reservoir of multiple species within the apicomplexan protozoan genus Theileria, including Theileria parva which causes East coast fever in cattle. A parasite, which has not yet been formally named, known as Theileria sp. (buffalo) has been r...
Bishop, Richard P.
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Hemmink, W.I.
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Morrison, J.D.
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Weir, W.
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Toye, Philip G.
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Sitt, T.
,
Spooner, P.R.
,
Musoke, A.J.
,
Skilton, Robert A.
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Odongo, David O.
,
[The African buffalo parasite Theileria. sp. (buffalo) can infect and immortalize cattle leukocytes and encodes divergent orthologues of Theileria parva antigen genes]
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The African buffalo parasite Theileria. sp. (buffalo) can infect and immortalize cattle leukocytes and encodes divergent orthologues of Theileria parva antigen genes
Previous studies using monoclonal antibody (mAb) 4C9 specific for a 32 kDa antigen (p32) of Theileria parva demonstrated expression of the antigen on the surface of the sporozoite, making it a potential antigen for sporozoite neutralization. A full-length cDNA encoding the major merozoite/piropla...
Skilton, Robert A.
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Musoke, A.J.
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Wells, C.W.
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Yagi, Y.
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Nene, Vishvanath
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Spooner, P.R.
,
Gachanja, J.
,
Osaso, J.
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Bishop, Richard P.
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Morzaria, S.P.
,
[A 32 kDa surface antigen of Theileria parva: Characterization and immunization studies]
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A 32 kDa surface antigen of Theileria parva: Characterization and immunization studies
Dobbelaere, D.A.E.
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Spooner, P.R.
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Barry, W.C.
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Irvin, A.D.
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[Monoclonal antibody neutralizes the sporozoite stage of different Theileria parva stocks]
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Monoclonal antibody neutralizes the sporozoite stage of different Theileria parva stocks
A 3-step procedure for cloning Theileria parva parasites was developed. The first step involved the in vitro infection of a fixed number of bovine lymphocytes with titrated sporozoites. The cell lines obtained from infections initiated using sporozoite/lymphocyte ratios below 1:100 were then sele...
Morzaria, S.P.
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Dolan, T.T.
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Norval, R.A.I.
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Bishop, Richard P.
,
Spooner, P.R.
,
[Generation and characterisation of cloned Theileria parva parasites]
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Generation and characterisation of cloned Theileria parva parasites
Allsopp, B.A.
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Bayles, H.A.
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Allsopp, M.T.
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Cavalier-Smith, T.
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Bishop, Richard P.
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Carrington, D.M.
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Sohanpal, B.K.
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Spooner, P.R.
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[Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences]
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Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences
A hybridoma cell line has been previously produced which secretes monoclonal antibodies that neutralize sporozoites of T. parva. Cells from this line were injected i/p into pristane-treated BALB/c mice. During the last 4 days of hybridoma cell growth, mice were given 4 daily i/p injections of a m...
Dobbelaere, D.A.E.
,
Spooner, P.R.
,
[Production in ascites fluid of biosynthetically labelled monoclonal antibody to Theileria parva sporozoites]
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Production in ascites fluid of biosynthetically labelled monoclonal antibody to Theileria parva sporozoites
Mini- and microsatellite sequences show high levels of variation and therefore provide excellent tools for both the genotyping and population genetic analysis of parasites. Herein we describe the identification of a panel of 11 polymorphic microsatellites and 49 polymorphic minisatellites of th...
Oura, C.A.L.
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Odongo, David O.
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Lubega, G.W.
,
Spooner, P.R.
,
Tait, A.
,
Bishop, Richard P.
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[A panel of microsatellite and minisatellite markers for the characterisation of field isolates of Theileria parva]
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A panel of microsatellite and minisatellite markers for the characterisation of field isolates of Theileria parva
Bishop, Richard P.
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Sohanpal, B.K.
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Kariuki, D.
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Young, A.S.
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Nene, Vishvanath
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Baylis, H.
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Allsopp, B.A.
,
Spooner, P.R.
,
Dolan, T.T.
,
Morzaria, S.P.
,
[Detection of a carrier state in Theileria parva infected cattle using the polymerase chain reaction]
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Detection of a carrier state in Theileria parva infected cattle using the polymerase chain reaction