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Objectives: The objective of this study was to assess whether cytotoxic T cells (CTL) generated by the live vaccine, known as “ITM Muguga cocktail”, which is used for the cattle disease East Cost fever (ECF) in Sub-Saharan Africa, showed a broad reactivity against many different strains of the ca...


Steinaa, LucillaSvitek, NicholasAwino, EliasSaya, RosemaryToye, Philip G.[Cytotoxic T lymphocytes from cattle sharing the same MHC class I haplotype and immunized with live Theileria parva sporozoites differ in antigenic specificity]Cytotoxic T lymphocytes from cattle sharing the same MHC class I haplotype and immunized with live Theileria parva sporozoites differ in antigenic specificity

Tremendous progress has been made over the last ten years on East Coast fever (ECF) research. Publication of a reference genome sequence of Theileria parva, the causative agent of ECF, has led to a more thorough characterization of the genotypic and antigenic diversity of the pathogen. It also fa...


Nene, VishvanathKiara, Henry K.Lacasta, AnnaPelle, RogerSvitek, NicholasSteinaa, Lucilla[The biology of Theileria parva and control of East Coast fever—Current status and future trends]The biology of Theileria parva and control of East Coast fever—Current status and future trends

We have recently shown that the BoLA-A18 variant haplotype (BoLA-6*01302) is more prevalent than the BoLA-A18 haplotype (BoLA-6*01301) in a sample of Holstein/Friesian cattle in Kenya. These MHC class I allelic variants differ by a single amino acid polymorphism (Glu97 to Leu97) in the peptide-bi...


Svitek, NicholasAwino, EliasNene, VishvanathSteinaa, Lucilla[BoLA-6*01301 and BoLA-6*01302, two allelic variants of the A18 haplotype, present the same epitope from the Tp1 antigen of Theileria parva]BoLA-6*01301 and BoLA-6*01302, two allelic variants of the A18 haplotype, present the same epitope from the Tp1 antigen of Theileria parva

This study was conducted to investigate the genetic diversity at d-loop of mitochondrial DNA and establish the possible maternal lineages of indigenous goat breeds in Democratic Republic of Congo (DRCongo). Phylogenetic relationships among and within 111 goats from three indigenous populations fr...


Bwihangane, B.A.Gitao, G.C.Bebora, L.C.Tarekegn, M.G.Nicholas, S.Bacigale, Samy B.Svitek, Nicholas[Mitochondrial DNA variation of indigenous goat populations from Peste-des-petits-ruminants outbreak in South Kivu, Democratic Republic of the Congo]Mitochondrial DNA variation of indigenous goat populations from Peste-des-petits-ruminants outbreak in South Kivu, Democratic Republic of the Congo

We have developed a polymerase chain reaction-sequence-specific primers-restriction fragment length polymorphism (PCR-SSP-RFLP) method to rapidly differentiate between the A18 and A18 variant (v) BoLA haplotypes and between A14 and A15/A15v BoLA haplotypes in Holstein/Friesian cattle. We used pub...


Svitek, NicholasNzau, Benjamin M.Steinaa, LucillaNene, Vishvanath[A method to discriminate between closely related bovine major histocompatibility complex class I alleles by combining established PCR-SSP assays with RFLPs]A method to discriminate between closely related bovine major histocompatibility complex class I alleles by combining established PCR-SSP assays with RFLPs

T cell responses contribute to immunity against many intracellular infections. There is, for example, strong evidence that major histocompatibility complex (MHC) class I-restricted cytotoxic T lymphocytes (CTLs) play an essential role in mediating immunity to East Coast fever (ECF), a fatal lymph...


Nene, VishvanathSvitek, NicholasToye, Philip G.Golde, W.T.Barlow, J.Buus, SorenNielsen, Morten[Designing bovine T cell vaccines via reverse immunology]Designing bovine T cell vaccines via reverse immunology

Peptide-major histocompatibility complex (p-MHC) class I tetramer complexes have facilitated the early detection and functional characterisation of epitope specific CD8+ cytotoxic T lymphocytes (CTL). Here, we report on the generation of seven recombinant bovine leukocyte antigens (BoLA) and reco...


Svitek, NicholasHansen, A.M.Steinaa, LucillaSaya, R.Awino, EliasNielsen, MortenBuus, SorenNene, Vishvanath[Use of “one-pot, mix-and-read” peptide-MHC class I tetramers and predictive algorithms to improve detection of cytotoxic T lymphocyte responses in cattle]Use of “one-pot, mix-and-read” peptide-MHC class I tetramers and predictive algorithms to improve detection of cytotoxic T lymphocyte responses in cattle
Nyagwange, JamesAwino, EliasTijhaar, EdwinSvitek, NicholasPelle, RogerNene, Vishvanath[Leveraging the Medicines for Malaria Venture malaria and pathogen boxes to discover chemical inhibitors of East Coast fever]Leveraging the Medicines for Malaria Venture malaria and pathogen boxes to discover chemical inhibitors of East Coast fever

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