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The concentration of African cassava mosaic virus (ACMV) was assessed by enzyme-linked immunosorbent assay in relation to symptom severity among resistant, moderately resistant and susceptible cassava genotypes. Resistant genotype NR 8083 had significantly lower symptom severity scores (P < 0·05)...


Ogbe, F.Atiri, G.Dixon, Alfred G.O.Thottappilly, G.[Symtom severity of cassava mosaic disease in relation to concentration of African cassava mosaic virus in different cassava genotypes]Symtom severity of cassava mosaic disease in relation to concentration of African cassava mosaic virus in different cassava genotypes

Eight viruses are reported from cowpeas in Africa. Cowpea viruses are described from lowland, humid and sub-humid West Africa, as well as from mid-altitude ecological zones mainly found in eastern and southern Africa. Wherever facilities are limited and detailed characterization is not possible, ...


Thottappilly, G.Rossel, H.W.[Virus diseases of cowpea in Africa: IITA Research Guide, No. 53]Virus diseases of cowpea in Africa: IITA Research Guide, No. 53

A Nigerian isolate of Yam mosaic virus (YMV), genus Potyvirus from Dioscorea rotundata leaves was used to mechanically inoculate Nicotiana benthamiana (propagation host) plants from which the virus was purified. A hybridoma cell line secreting a monoclonal antibody (Mab) against YMV was produced ...


Njukeng, A.P.Ariri, G.I.Hughes, Jacqueline d'ArrosAgindoran, B.O.Mignouna, H.D.Thottappilly, G.[A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp.]A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp.

Reliable characterization of the variation among wild and cultivated yams in Nigeria is essential for improved management and efficient utilization of yam genetic resources. RAPD and double stringency PCR (DS-PCR) analyses were used to investigate genetic relationships and the extent of redundanc...


Mignouna, H.Abang, Mathew M.Wanyera, N.Chikaleke, V.Asiedu, RobertThottappilly, G.[PCR markerbased analysis of wild and cultivated yams (Dioscorea spp.)in Nigeria: genetic relationships and implication for ex situ conservation]PCR markerbased analysis of wild and cultivated yams (Dioscorea spp.)in Nigeria: genetic relationships and implication for ex situ conservation

Vigna vexillata is a wild cross-incompatible relative of cowpea. It is highly resistant to several diseases and pests plaguing cowpea. A linkage map was developed for V. vexillata comprising 120 markers, including 70 random amplified polymorphic DNAs, 47 amplified fragment length polymorphisms, o...


Ogundiwin, E.Thottappilly, G.Akenova, M.E.Pillay, M.Fatokun, C.A.[A genetic linkage map of Vigna vexillata]A genetic linkage map of Vigna vexillata

A survey was carried out in the 1996/97 and 1997/98 growing seasons on a field planted in three replicates with five clones of cassava at the International Institute of Tropical Agriculture, Ibadan, located in a transition forest, to determine the effects of cassava genotype and climate on the de...


Abdullahi, I.Atiri, G.I.Dixon, Alfred G.O.Winter, S.Thottappilly, G.[Effects of cassava genotype, climate, and the Bemisia tabaci vector population on the development of African cassava mosaic geminivirus (ACMV)]Effects of cassava genotype, climate, and the Bemisia tabaci vector population on the development of African cassava mosaic geminivirus (ACMV)

Maize (Zea mays) and itch grass (Rottboellia cochinchinensis) plants exhibiting a mild mosaic or mottle were collected from a farmer's field near Mokwa, Nigeria, in 1993. Icosahedral virions (approximately 28 to 30 nm) were purified from symptomatic tissue by differential centrifugation in 0.1 M ...


Thottappilly, G.Qiu, W.P.Batten, J.S.Hughes, J.N.Scholthof, K.B.[A new virus on maize in Nigeria: maize mild mottle virus]A new virus on maize in Nigeria: maize mild mottle virus

DNA was extracted from the mycelia of Colletotrichum gloeosporioides[Glomerella cingulata] isolates from yam, and amplification reactions using 11 random primers generated 116 DNA bands, 70 of them being informative. The electrophoretic patterns revealed by these primers allowed the identificatio...


Thottappilly, G.Mignouna, H.D.Onasanya, A.Abang, Mathew M.Oyelakin, O.Singh, N.K.[Identification and differentiation of isolates of Colletotrichum gloeosporioides from yam by random amplified polymorphic DNA markers]Identification and differentiation of isolates of Colletotrichum gloeosporioides from yam by random amplified polymorphic DNA markers

Both components of the virus complex that causes the sweet potato virus disease (SPVD) in Nigeria can individually be transmitted by their respective vectors from diseased sweet potato to healthy sweet potato and to the test plant, Ipomoea setosa. But in isolation, neither the aphid nor the white...


Rossel, H.W.Thottappilly, G.[Complex virus diseases of sweet potato]Complex virus diseases of sweet potato

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