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Ballén-Taborda, CarolinaPlata, GermánAyling, SarahRodríguez-Zapata, FaustoBecerra López Lavelle, Luis AugustoDuitama, JorgeTohme, Joseph M.[Identification of Cassava MicroRNAs under abiotic stress]Identification of Cassava MicroRNAs under abiotic stress

Genetic analysis based on Random Amplified Polymorphic DNA (RAPD) was carried out on 52 accessions representing 14 species of the genus Passiflora L. using 50 random 10-mer primers. A dendrogram constructed using the Dice similarity coefficient and the UPGMA algorithm based on 626 reproducible po...


Fajardo, DiegoAngel, F.Grum, M.Tohme, Joseph M.Lobo Arias, MRoca, William M.Sánchez, I.[Genetic variation analysis of the genus Passiflora L. using RAPD markers]Genetic variation analysis of the genus Passiflora L. using RAPD markers

Cassava bacterial blight (CBB), caused by Xanthomonas axonopodis pv. manihotis (Xam), is a major disease of cassava (Manihot esculenta Crantz) in Africa and South America. Planting resistant varieties is the preferred method of disease control. Recent genetic mapping of an F1 cross (TMS 30572 × C...


Jorge, VFregene, Martin A.Duque E., Myriam CristinaBonierbale, Merideth W.Tohme, Joseph M.Verdier, Valérie[Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz)]Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz)

The extent and structure of the genetic variability of traditional varieties of cassava (Manihot esculenta Crantz) have been little documented, despite considerable evidence for this crop's great varietal diversity in traditional agroecosystems. We used microsatellite markers to assess the geneti...


Elias, M.Muhlen, GSMcKey, DRoa, ACTohme, Joseph M.[Genetic diversity of traditional South American landraces of cassava (Manihot esculenta Crantz): An analysis using microsatellites]Genetic diversity of traditional South American landraces of cassava (Manihot esculenta Crantz): An analysis using microsatellites

Microsatellite polymorphism was studied in a sample of 39 traditional rice (Oryza sativa L.) varieties and 11 improved varieties widely planted in Cuba. The study was aimed at assessing the extent of genetic variation in traditional and improved varieties and to establish their genetic relationsh...


Álvarez, A.Fuentes, JLPuldon, VGómez, P.J.Mora, LDuque E., Myriam CristinaGallego Sánchez, Gerardo J.Tohme, Joseph M.[Genetic diversity analysis of Cuban traditional rice (Oryza sativa L.) varieties based on microsatellite markers]Genetic diversity analysis of Cuban traditional rice (Oryza sativa L.) varieties based on microsatellite markers

Traditional breeding efforts are expected to be greatly enhanced through collaborative approaches incorporating functional, comparative and structural genomics. Potential benefits of combining genomic tools with traditional breeding have been a source of widespread interest and resulted in numero...


Ishitani, ManabuRao, Idupulapati M.Wenzl, PeterBeebe, Stephen E.Tohme, Joseph M.[Integration of genomics approach with traditional breeding towards improving abiotic stress adaptation: Drought and aluminum toxicity as case studies]Integration of genomics approach with traditional breeding towards improving abiotic stress adaptation: Drought and aluminum toxicity as case studies

A major constraint for incorporating new traits into cassava using biotechnology is the limited list of known/tested promoters that encourage the expression of transgenes in the cassava’s starchy roots. Based on a previous report on the glutamic-acid-rich protein Pt2L4, indicating a preferential ...


Beltrán Giraldo, Jorge AlonsoPrias, MAl-Babili, S.Ladino Franco, YJLópez, D.Beyer, PeterChavarriaga Aguirre, PaulTohme, Joseph M.[Expression pattern conferred by a glutamic acid-rich protein gene promoter in field-grown transgenic cassava (Manihot esculenta Crantz)]Expression pattern conferred by a glutamic acid-rich protein gene promoter in field-grown transgenic cassava (Manihot esculenta Crantz)

Breeding goals of yield increases, root quality improvement, and disease resistance in cassava are considerably slowed down by biological characteristics of the crop, which includes a long growth cycle, a heterozygous genetic background and a poor knowledge of the organization of crop diversity. ...


Fregene, Martin A.Okogbenin, EmmanuelMba, CAngel, F.Suarez, MCGutiérrez A., Janeth PatriciaChavarriaga Aguirre, PaulRoca, William M.Bonierbale, Merideth W.Tohme, Joseph M.[Genome mapping in cassava improvement: Challenges, achievements and opportunities]Genome mapping in cassava improvement: Challenges, achievements and opportunities

Xanthomonas oryzae pathovar oryzae (Xoo) and X. oryzae pv. oryzicola (Xoc) cause bacterial diseases in rice: leaf blight and leaf streak, respectively. Although both the Asian and the African strains of Xoo induce similar symptoms, they are genetically different, with the African Xoo strains bein...


Soto Suárez, M.González, C.Piegu, BTohme, Joseph M.Verdier, Valérie[Genomic comparison between Xanthomonas oryzae pv.oryzae and Xanthomonas oryzae pv.oryzicola,usingsuppression-subtractive hybridization]Genomic comparison between Xanthomonas oryzae pv.oryzae and Xanthomonas oryzae pv.oryzicola,usingsuppression-subtractive hybridization

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