We applied the cDNA-AFLP (amplified fragment length polymorphism) technique to mRNA from the parents of a cassava (Manihot esculenta) genetic mapping population, and obtained more than 500 transcript-derived fragments (TDFs) that were unique in either parent. A subset of 50 TDFs were cloned and s...
Suarez, MC
,
Bernal, A.
,
Gutiérrez, J.
,
Tohme, Joseph M.
,
Fregene, Martin A.
,
[Developing expressed sequence tags (ESTs) from polymorphic transcript-derived fragments (TDFs) in cassava (Manihot esculenta Crantz)]
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Developing expressed sequence tags (ESTs) from polymorphic transcript-derived fragments (TDFs) in cassava (Manihot esculenta Crantz)
More than 60% of common bean production worldwide is derived from cultivars of Middle American origin. Understanding the diversity of these will facilitate their use in genetic improvement. The objective of this study was to analyze a collection of 269 landraces of common bean (Phaseolus vulgaris...
Beebe, Stephen E.
,
Skroch, PW
,
Tohme, Joseph M.
,
Duque E., Myriam Cristina
,
Pedraza García, Fabio
,
Nienhuis, James
,
[Structure of genetic diversity among common bean landraces of middle American origin based on correspondence analysis of RAPD]
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Structure of genetic diversity among common bean landraces of middle American origin based on correspondence analysis of RAPD
A polymerase chain reaction approach using degenerate primers that targeted the conserved domains of cloned plant disease resistance genes (R genes) was used to isolate a set of 15 resistance gene analogs (RGAs) from common bean (Phaseolus vulgaris). Eight different classes of RGAs were obtained ...
López, C.E.
,
Acosta, I.F.
,
Jara, Carlos E.
,
Pedraza García, Fabio
,
Gaitán Solís, Eliana
,
Gallego Sánchez, Gerardo J.
,
Beebe, Stephen E.
,
Tohme, Joseph M.
,
[Identifying resistance gene analogs associated with resistances to different pathogens in common beans]
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Identifying resistance gene analogs associated with resistances to different pathogens in common beans
DNA variation was studied at the intra- andinterspecific levels within the genus Passiflora, with particular emphasis on the subgenusTacsonia and its relationship with the subgenusPassiflora. AFLP fingerprints were generated for35 accessions from 21 species, 14 of which belong to subgenusTacsonia...
Segura, Sergio D.
,
Coppens d'Eeckenbrugge, Geo
,
Bohórquez Cháux, Adriana
,
Ollitrault, P
,
Tohme, Joseph M.
,
[An AFLP diversity study of the genus Passiflora focusing on subgenus Tacsonia]
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An AFLP diversity study of the genus Passiflora focusing on subgenus Tacsonia
In Venezuela, pedigree analyses indicate that the rice varieties currently under cultivation are closely related. Effective breeding programs, based on knowledge of the genetic diversity of cultivars, are needed to broaden the genetic bases of rice germplasm in the country. In this study, we used...
Ghneim-Herrera, Thaura
,
Posso Duque, D
,
Pérez Almeida, I.
,
Torrealba N., G.T.
,
Pieters, AJ
,
Martínez Racines, César Pompilio
,
Tohme, Joseph M.
,
[Assessment of genetic diversity in Venezuelan rice cultivars using simple sequence repeats markers]
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Assessment of genetic diversity in Venezuelan rice cultivars using simple sequence repeats markers
A total of 150 microsatellite markers developed for common bean (Phaseolus vulgaris L.) were tested for parental polymorphism and used to determine the positions of 100 genetic loci on an integrated genetic map of the species. The value of these single-copy markers was evident in their ability to...
Blair, Matthew W.
,
Pedraza García, Fabio
,
Buendía Castellanos, Hector F.
,
Gaitán Solís, Eliana
,
Beebe, Stephen E.
,
Gepts, Paul L.
,
Tohme, Joseph M.
,
[Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.)]
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Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.)
A cassava cDNA microarray based on a large cassava EST database was constructed and used to study the incompatible interaction between cassava and Xanthomonas axonopodis pv. manihotis (Xam) strain CIO151. For microarray construction, 5700 clones from the cassava unigene set were amplified by poly...
López, C.E.
,
Soto Suárez, M.
,
Restrepo, S.
,
Piegu, B
,
Cooke, RG
,
Delseny, M
,
Tohme, Joseph M.
,
Verdier, Valérie
,
[Gene expression profile in response to Xanthomonas axonopodis pv. manihotis infection in cassava using a cDNA microarray]
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Gene expression profile in response to Xanthomonas axonopodis pv. manihotis infection in cassava using a cDNA microarray
Cassava (Manihot esculenta Crantz) germplasm collections are conventionally maintained by continuous vegetative propagation in the field. Tissue culture techniques provide a more convenient way to conserve germplasm. The cassava in vitro gene bank held in trust at CIAT comprises nearly 6000 acces...
Angel, F.
,
Barney, Victoria E.
,
Tohme, Joseph M.
,
Roca, William M.
,
[Stability of cassava plants at the DNA level after retrieval from 10 years of in vitro storage]
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Stability of cassava plants at the DNA level after retrieval from 10 years of in vitro storage
Fory Sánchez, L.F.
,
Pérez, J.G.
,
Duque, S.
,
Calle, F
,
Morante, N
,
Bolaños, E.
,
Herrera, P.
,
Duque, M.
,
Ceballos, H.
,
Roca, William M.
,
Gallego Sánchez, Gerardo J.
,
Tohme, Joseph M.
,
[Linea base para la toma de decisiones en bioseguridad: desarrollo de un modelo para la evaluación de flujo de genes en yuca utilizando una linea androesteril (Manihot esculenta Crantz)]
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Linea base para la toma de decisiones en bioseguridad: desarrollo de un modelo para la evaluación de flujo de genes en yuca utilizando una linea androesteril (Manihot esculenta Crantz)
Pérez, J.C.
,
Fory Sánchez, L.F.
,
Velásquez, A.
,
Duque, S.
,
Herrera, P.
,
Calle, F
,
Morante, N
,
Duque, M.
,
Ceballos, H.
,
Roca, W.
,
Tohme, Joseph M.
,
Gallego Sánchez, Gerardo J.
,
[Uso de marcadores SNPs y SSR para la evaluación de flujo de genes en yuca (Manihot esculenta Crantz)]
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Uso de marcadores SNPs y SSR para la evaluación de flujo de genes en yuca (Manihot esculenta Crantz)