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A polymerase chain reaction approach using degenerate primers that targeted the conserved domains of cloned plant disease resistance genes (R genes) was used to isolate a set of 15 resistance gene analogs (RGAs) from common bean (Phaseolus vulgaris). Eight different classes of RGAs were obtained ...


López, C.E.Acosta, I.F.Jara, Carlos E.Pedraza García, FabioGaitán Solís, ElianaGallego Sánchez, Gerardo J.Beebe, Stephen E.Tohme, Joseph M.[Identifying resistance gene analogs associated with resistances to different pathogens in common beans]Identifying resistance gene analogs associated with resistances to different pathogens in common beans

DNA variation was studied at the intra- andinterspecific levels within the genus Passiflora, with particular emphasis on the subgenusTacsonia and its relationship with the subgenusPassiflora. AFLP fingerprints were generated for35 accessions from 21 species, 14 of which belong to subgenusTacsonia...


Segura, Sergio D.Coppens d'Eeckenbrugge, GeoBohórquez Cháux, AdrianaOllitrault, PTohme, Joseph M.[An AFLP diversity study of the genus Passiflora focusing on subgenus Tacsonia]An AFLP diversity study of the genus Passiflora focusing on subgenus Tacsonia

In Venezuela, pedigree analyses indicate that the rice varieties currently under cultivation are closely related. Effective breeding programs, based on knowledge of the genetic diversity of cultivars, are needed to broaden the genetic bases of rice germplasm in the country. In this study, we used...


Ghneim-Herrera, ThauraPosso Duque, DPérez Almeida, I.Torrealba N., G.T.Pieters, AJMartínez Racines, César PompilioTohme, Joseph M.[Assessment of genetic diversity in Venezuelan rice cultivars using simple sequence repeats markers]Assessment of genetic diversity in Venezuelan rice cultivars using simple sequence repeats markers

A total of 150 microsatellite markers developed for common bean (Phaseolus vulgaris L.) were tested for parental polymorphism and used to determine the positions of 100 genetic loci on an integrated genetic map of the species. The value of these single-copy markers was evident in their ability to...


Blair, Matthew W.Pedraza García, FabioBuendía Castellanos, Hector F.Gaitán Solís, ElianaBeebe, Stephen E.Gepts, Paul L.Tohme, Joseph M.[Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.)]Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.)

A cassava cDNA microarray based on a large cassava EST database was constructed and used to study the incompatible interaction between cassava and Xanthomonas axonopodis pv. manihotis (Xam) strain CIO151. For microarray construction, 5700 clones from the cassava unigene set were amplified by poly...


López, C.E.Soto Suárez, M.Restrepo, S.Piegu, BCooke, RGDelseny, MTohme, Joseph M.Verdier, Valérie[Gene expression profile in response to Xanthomonas axonopodis pv. manihotis infection in cassava using a cDNA microarray]Gene expression profile in response to Xanthomonas axonopodis pv. manihotis infection in cassava using a cDNA microarray

Cassava (Manihot esculenta Crantz) germplasm collections are conventionally maintained by continuous vegetative propagation in the field. Tissue culture techniques provide a more convenient way to conserve germplasm. The cassava in vitro gene bank held in trust at CIAT comprises nearly 6000 acces...


Angel, F.Barney, Victoria E.Tohme, Joseph M.Roca, William M.[Stability of cassava plants at the DNA level after retrieval from 10 years of in vitro storage]Stability of cassava plants at the DNA level after retrieval from 10 years of in vitro storage

Fory Sánchez, L.F.Pérez, J.G.Duque, S.Calle, FMorante, NBolaños, E.Herrera, P.Duque, M.Ceballos, H.Roca, William M.Gallego Sánchez, Gerardo J.Tohme, Joseph M.[Linea base para la toma de decisiones en bioseguridad: desarrollo de un modelo para la evaluación de flujo de genes en yuca utilizando una linea androesteril (Manihot esculenta Crantz)]Linea base para la toma de decisiones en bioseguridad: desarrollo de un modelo para la evaluación de flujo de genes en yuca utilizando una linea androesteril (Manihot esculenta Crantz)

Pérez, J.C.Fory Sánchez, L.F.Velásquez, A.Duque, S.Herrera, P.Calle, FMorante, NDuque, M.Ceballos, H.Roca, W.Tohme, Joseph M.Gallego Sánchez, Gerardo J.[Uso de marcadores SNPs y SSR para la evaluación de flujo de genes en yuca (Manihot esculenta Crantz)]Uso de marcadores SNPs y SSR para la evaluación de flujo de genes en yuca (Manihot esculenta Crantz)

Para marcar los loci que determinan la resistencia al ataque de la bacteriosis común en frijol, se emplearon los polimorfismos en el ADN aleatoriamente amplificado (RAPD), junto con tres diferente metodológicas de análisis génico: análisis de los segregantes diferencialmente agrupados (BSA), sele...


Pedraza García, FabioGallego Sánchez, Gerardo J.Beebe, Stephen E.Tohme, Joseph M.[Marcadores SCAR y RAPD para la resistencia a la bacteriosis común (CBB) del frijol]Marcadores SCAR y RAPD para la resistencia a la bacteriosis común (CBB) del frijol
Sanabria, YCarabalí, Silvio J.Olaya, Cristian A.Martínez Racines, César PompilioTohme, Joseph M.[Using molecular markers to search for wild introgressions from a relative tetraploid species into Oryza sativa L.]Using molecular markers to search for wild introgressions from a relative tetraploid species into Oryza sativa L.

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