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The angiosperm circadian clock has been well established from molecular-genetic studies in a temperate plant model. Conservation of clock function is less explored in plants from the tropics. Cassava (Manihot esculenta) is a staple crop grown in the tropics that has been of limited research inter...


Adeyemo, O. SarahKolmos, ETohme, Joseph M.Chavarriaga Aguirre, PaulFregene, Martin A.Davis, S.J.[Identification and characterization of the Cassava core-clock gene Early Flowering 4]Identification and characterization of the Cassava core-clock gene Early Flowering 4

More than 60% of common bean production worldwide is derived from cultivars of Middle American origin. Understanding the diversity of these will facilitate their use in genetic improvement. The objective of this study was to analyze a collection of 269 landraces of common bean (Phaseolus vulgaris...


Beebe, Stephen E.Skroch, PWTohme, Joseph M.Duque E., Myriam CristinaPedraza García, FabioNienhuis, James[Structure of genetic diversity among common bean landraces of middle American origin based on correspondence analysis of RAPD]Structure of genetic diversity among common bean landraces of middle American origin based on correspondence analysis of RAPD

A polymerase chain reaction approach using degenerate primers that targeted the conserved domains of cloned plant disease resistance genes (R genes) was used to isolate a set of 15 resistance gene analogs (RGAs) from common bean (Phaseolus vulgaris). Eight different classes of RGAs were obtained ...


López, C.E.Acosta, I.F.Jara, Carlos E.Pedraza García, FabioGaitán Solís, ElianaGallego Sánchez, Gerardo J.Beebe, Stephen E.Tohme, Joseph M.[Identifying resistance gene analogs associated with resistances to different pathogens in common beans]Identifying resistance gene analogs associated with resistances to different pathogens in common beans

Cassava (Manihot esculenta) is a major food staple for nearly 600 million people in Africa, Asia, and Latin America. Major losses in yield result from biotic and abiotic stresses that include diseases such as Cassava Mosaic Disease (CMD) and Cassava Bacterial Blight (CBB), drought, and acid soils...


Anderson, J.W.Delseny, MFregene, Martin A.Jorge, VMba, CLópez, C.E.Restrepo, S.Soto Suárez, M.Piegu, BVerdier, ValérieCooke, RGTohme, Joseph M.Horvat, DP[An EST resource for cassava and other species of Euphorbiaceae]An EST resource for cassava and other species of Euphorbiaceae

DNA variation was studied at the intra- andinterspecific levels within the genus Passiflora, with particular emphasis on the subgenusTacsonia and its relationship with the subgenusPassiflora. AFLP fingerprints were generated for35 accessions from 21 species, 14 of which belong to subgenusTacsonia...


Segura, Sergio D.Coppens d'Eeckenbrugge, GeoBohórquez Cháux, AdrianaOllitrault, PTohme, Joseph M.[An AFLP diversity study of the genus Passiflora focusing on subgenus Tacsonia]An AFLP diversity study of the genus Passiflora focusing on subgenus Tacsonia

The present work was conducted to identify microsatellite markers linked to the rice blast resistance gene Pi-1(t) for a marker-assisted selection program. Twenty-four primer pairs corresponding to 19 microsatellite loci were selected from the Gramene database (www. gramene.org) considering their...


Fuentes, JLCorrea Victoria, Fernando J.Escobar, F.Prado Patiño, G.A.Aricapa, María GirlenaDuque E., Myriam CristinaTohme, Joseph M.[Identification of microsatellite markers linked to the blast resistance gene Pi-1(t) in rice]Identification of microsatellite markers linked to the blast resistance gene Pi-1(t) in rice

Background: Bacterial leaf blight causes significant yield losses in rice crops throughout Asia and Africa. Although both the Asian and African strains of the pathogen, Xanthomonas oryzae pv. oryzae (Xoo), induce similar symptoms, they are nevertheless genetically different, with the African stra...


Soto Suárez, M.Bernal, D.González, C.Szurek, BGuyot, RTohme, Joseph M.Verdier, Valérie[In planta gene expression analysis of Xanthomonas oryzae pathovar oryzae, African strain MAI1]In planta gene expression analysis of Xanthomonas oryzae pathovar oryzae, African strain MAI1

In Venezuela, pedigree analyses indicate that the rice varieties currently under cultivation are closely related. Effective breeding programs, based on knowledge of the genetic diversity of cultivars, are needed to broaden the genetic bases of rice germplasm in the country. In this study, we used...


Ghneim-Herrera, ThauraPosso Duque, DPérez Almeida, I.Torrealba N., G.T.Pieters, AJMartínez Racines, César PompilioTohme, Joseph M.[Assessment of genetic diversity in Venezuelan rice cultivars using simple sequence repeats markers]Assessment of genetic diversity in Venezuelan rice cultivars using simple sequence repeats markers

A total of 150 microsatellite markers developed for common bean (Phaseolus vulgaris L.) were tested for parental polymorphism and used to determine the positions of 100 genetic loci on an integrated genetic map of the species. The value of these single-copy markers was evident in their ability to...


Blair, Matthew W.Pedraza García, FabioBuendía Castellanos, Hector F.Gaitán Solís, ElianaBeebe, Stephen E.Gepts, Paul L.Tohme, Joseph M.[Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.)]Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.)

A cassava cDNA microarray based on a large cassava EST database was constructed and used to study the incompatible interaction between cassava and Xanthomonas axonopodis pv. manihotis (Xam) strain CIO151. For microarray construction, 5700 clones from the cassava unigene set were amplified by poly...


López, C.E.Soto Suárez, M.Restrepo, S.Piegu, BCooke, RGDelseny, MTohme, Joseph M.Verdier, Valérie[Gene expression profile in response to Xanthomonas axonopodis pv. manihotis infection in cassava using a cDNA microarray]Gene expression profile in response to Xanthomonas axonopodis pv. manihotis infection in cassava using a cDNA microarray

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