El objetivo de esta revisión es analizar los factores que regulan o alteran la motilidad espermática en peces “de fecundación externa” antes y después del contacto con el agua para dar a conocer herramientas que optimicen las
metodologías utilizadas en piscicultura y así mejorar la capacidad fecu...
In most teleost fish with external fertilization, the spermatozoids are immobile in the seminal fluid and they are activated only after making contact with an aqueous medium. The time of flagellar movement is short in most species. Furthermore, the factors that trigger this process are different ...
Valdebenito Isler, Iván
,
Fletcher, C.
,
Vera, V.
,
Fernández, J.
,
[Physical-chemical factors that regulate spermatic motility in fish: basic and applied aspects. A review]
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[Factores fisicoquímicos que regulan la motilidad espermática en peces: aspectos básicos y aplicados. Una revisión]
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Factores fisicoquímicos que regulan la motilidad espermática en peces: aspectos básicos y aplicados. Una revisión
There is no information about the characteristics of early cleavage in the Patagonian blennie (Eleginops maclovinus), which can be used as a diagnostic tool for embryo quality. The purpose of this investigation, therefore, was to characterize the first blastomeres of E. maclovinus morphologically...
The aim of the present investigations was to test a novel technology comprising cryoprotectant-free vitrification of the spermatozoa of rainbow trout and to study the ability of sucrose and components of seminal plasma to protect these cells from cryoinjuries. Spermatozoa were isolated and vitrif...
Merino, O.
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Risopatrón, J.
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Sánchez, R.
,
Isachenko, E.
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Figueroa Villalobos, Elías
,
Valdebenito Isler, Iván
,
Isachenko, V.
,
[Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness]
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Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness
In terms of fish production, sperm activating substances are important in the controlled insemination. This holds also for salmonids, when being provided with an agent that energizes the spermatozoa. In this study two amino acids (l-glutamate and glycine) were evaluated in their effect on sperm m...
Valdebenito Isler, Iván
,
Moreno, C.
,
Lozano, C.
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Ubilla Madrid, Andrea
,
[Effect of l-glutamate and glycine incorporated in activation media, on sperm motility and fertilization rate of rainbow trout (Oncorhynchus mykiss) spermatozoa]
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Effect of l-glutamate and glycine incorporated in activation media, on sperm motility and fertilization rate of rainbow trout (Oncorhynchus mykiss) spermatozoa
In teleostei, as opposed to what happens in mammals, most of the studies that evaluate the quality storages of semen are oriented toward the exposure of spermatozoa to some reactive oxygen species (ROS), the utilization of antioxidants in the diet, or the incorporation of these in seminal plasma....
In fish feeding studies, stomach related parameters are mainly measured in order to have biometric relationships. To determine those parameters a direct and straightforward method for measuring real maximum stomach volume in fish based on Archimedes' principle was tested and compared against a tr...
Salgado Ismodes, Andres
,
Salgado Leu, Italo
,
Valdebenito Isler, Iván
,
[A direct and straightforward method for measurement real maximum fish stomach volume to improve aquaculture feeding research]
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A direct and straightforward method for measurement real maximum fish stomach volume to improve aquaculture feeding research
The present investigation determined how different antioxidants incorporated into the sperm diluent for cold storage of semen affected sperm motility and spermatozoan fertility capabilities of the rainbow trout. For the evaluations, fresh semen (C) and semen that had been stored without diluents ...
Ubilla Madrid, Andrea
,
Valdebenito Isler, Iván
,
[Use of antioxidants on rainbow trout Oncorhynchus mykiss (Walbaum, 1792) sperm diluent: effects on motility and fertilizing capability]
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Use of antioxidants on rainbow trout Oncorhynchus mykiss (Walbaum, 1792) sperm diluent: effects on motility and fertilizing capability
The quality of fish gametes, both male and female, are determined by several factors (age, management, feeding, chemical and physical factors, water quality, etc.)that have an impact on the survivability of embryos, larvae and/or fry in the short or long term. One of the most important factors is...
In this article we describe basic aspects of the sperm biology of Merluccius australis, in particular assessing the effects of pH (6, 8 and 10), temperature (4, 10 and 15 degrees C) and osmolality (922 mOsm/kg, 822 mOsm/kg, 681 mOsm/kg and 3 mOsm/kg) on sperm motility. Our results indicate the pr...
Effer Roldan, Brian
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Figueroa Villalobos, Elías
,
Augsburger, A.
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Valdebenito Isler, Iván
,
[Sperm biology of Merluccius australis: Sperm structure, semen characteristics and effects of pH, temperature and osmolality on sperm motility]
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Sperm biology of Merluccius australis: Sperm structure, semen characteristics and effects of pH, temperature and osmolality on sperm motility
Sperm cryopreservation is an important tool for reproductive management; however, it has not been possible to perform this procedure for coho salmon. This study was designed to assess the effect of cryopreservation on sperm functional parameters, oxidative stress markers and fertilizing capacity ...